Method for determining contents of seven components in lonicera and forsythia powder by adopting dual-wavelength quantitative analysis of multi-components by single marker
A measurement method and dual-wavelength technology, applied in measuring devices, material separation, and analysis of materials, can solve problems such as inability to comprehensively evaluate and single components
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Embodiment 1
[0036] (1) Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, forsythiaside A, isochlorogenic acid A, isochlorogenic acid C, forsythin, and arctiin reference substances, and use 50% The chromatographic methanol is configured as a mixed reference solution with a concentration of 7.7, 96, 296, 65, 15.2, 27.5, and 113 μg / ml;
[0037] (2) Preparation of the test solution: Accurately weigh 0.5g of Yinqiaosan, dilute to volume with 50% methanol aqueous solution, weigh, ultrasonicate for 30 minutes, let cool, make up for weight loss, filter, and take the filtrate to pass through 0.45μm organic micropores Filter the sample solution to be tested, and the concentration is 0.02g / ml; prepare negative samples lacking honeysuckle, forsythia, arctiin single medicinal material, and negative samples lacking honeysuckle and Arctium two medicinal materials respectively in the same way;
[0038] (3) Chromatographic conditions: chromatographic column Thermo ...
Embodiment 2
[0067] Take 10 batches of Yinqiao powder samples, prepare the test solution according to the method of step (2) of embodiment 1, inject samples according to the chromatographic conditions of step (3) of the embodiment, and record at 237nm, forsythiaside A, forsythin, Peak area of arctiin, peak area of forsythiaside A, neochlorogenic acid, chlorogenic acid (C), isochlorogenic acid A, and isochlorogenic acid C at 327nm.
[0068] Using the relative correction factor in Example 1, the external standard one-point method (ESM) and the one-measurement-multiple-evaluation method (QAMS) were used to calculate the content of each component of Yinqiao powder. The relative deviation of the results of the two methods of ESM and QAMS was -0.35 % to 0.11%, all less than 3% (see Table 6 and Table 7).
[0069] Table 6 Comparison of ESM and QAMS content determination results at 237nm
[0070] ;
[0071] Table 7 Comparison of ESM and QAMS calculation results at 327nm
[0072] .
[00...
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