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Lipid droplet targeting carbon dot and preparation method and application thereof

A technology of carbon dots and lipid droplets, applied in the field of fluorescent nanomaterials manufacturing, can solve problems such as limiting the in-depth research of carbon dots

Active Publication Date: 2021-07-06
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on lipid droplet-specific probes based on carbon dots, which greatly limits the in-depth study of carbon dots in the field of bioimaging.

Method used

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  • Lipid droplet targeting carbon dot and preparation method and application thereof
  • Lipid droplet targeting carbon dot and preparation method and application thereof
  • Lipid droplet targeting carbon dot and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for preparing carbon dots, comprising the following steps:

[0049] First weigh 50 mg of 4-(1-piperidinyl)aniline and dissolve it in 5 mL of absolute ethanol, then transfer the solution to a polytetrafluoroethylene-lined autoclave, and place the autoclave in an oven In a dry box, heated to 160 °C for 12 h. After the reaction was completed, the reactor was cooled to room temperature to obtain a brownish-red solution, and then the crude product was purified by silica gel column chromatography, using different proportions of petroleum ether and ethyl acetate as eluents, rotary evaporation and further drying, Purified fluorescent carbon dots were obtained.

Embodiment 2

[0055] Fluorescence intensity changes of carbon dots in different solvents

[0056] Add 2 mL of different solvents to the cuvette, add 4 μL of carbon dot stock solution, and measure the fluorescence spectrum. The experimental results show that: if figure 1 As shown, the fluorescence of carbon dots in aqueous solution is very weak and negligible, which has the potential for wash-free cell imaging.

Embodiment 3

[0058] Fluorescence intensity changes in 1,4-dioxane solutions with different water contents (0-100%)

[0059] Add 2 mL of 1,4-dioxane solution with different water content (0-100%) to the cuvette, add 4 μL of carbon dot stock solution, and measure the fluorescence spectrum. The experimental results show that: if figure 2 As shown, the fluorescence of carbon dots in pure 1,4-dioxane solution is very strong, which is conducive to the targeting of carbon dots to lipid droplets. Moreover, the fluorescence of carbon dots in aqueous solution is very weak and can be ignored, which has the potential of no-wash lipid droplet imaging.

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Abstract

The invention provides a lipid droplet targeting carbon dot and a preparation method and application thereof. The fluorescent carbon dot takes 4-(1-piperidyl) aniline as a raw material and absolute ethyl alcohol as a solvent, the raw material and the solvent react in a high-pressure reaction kettle at the temperature of 160 DEG C for 12 h, a crude product obtained after the reaction is purified through silica gel column chromatography, petroleum ether and ethyl acetate in different proportions are taken as eluents, and rotary evaporation and further drying are carried out to obtain the purified fluorescent carbon dot. The carbon dots can quickly penetrate through cell membranes within 10 seconds and gather in lipid droplets, and can be used for ultrafast no-wash lipid droplet imaging in living cells and non-alcoholic fatty liver changes caused by bisphenol A. The fluorescent carbon dot is very simple to synthesize and purify, has the advantages of good light stability, large Stokes shift, high signal-to-noise ratio and the like, can quickly enter cells, and can naturally target lipid droplets.

Description

technical field [0001] The invention relates to the field of fluorescent nanomaterial manufacturing, in particular to a lipid droplet-targeted carbon dot, a preparation method and an application. Background technique [0002] Lipid droplets are dynamic spherical organelles composed of a hydrophobic core composed of various neutral lipids (such as triglycerides and cholesteryl esters) and a phospholipid monolayer. It plays an indispensable role in various physiological processes, such as: Lipid metabolism and storage, signal transduction, apoptosis, protein degradation, etc. Once abnormal accumulation of lipid droplets will lead to the risk of fatty liver, obesity, diabetes, and even cancer. Therefore, imaging lipid droplets is very necessary, which is very meaningful for studying the function of lipid droplets under normal or physiological and pathological conditions, and also provides useful information for early clinical diagnosis of lipid droplet-related diseases. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15G01N21/64B82Y20/00B82Y40/00
CPCC09K11/65G01N21/6428C01B32/15B82Y20/00B82Y40/00
Inventor 李朝辉王军丽孙远强杨冉屈凌波
Owner ZHENGZHOU UNIV
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