Method for culturing pig hair papilla cells

Abstract

The invention relates to a method for culturing pig hair papilla cells. The pig hair papilla cells are taken from pig ear skin tissues, I-type collagenase and pancreatin are used for digestion and separation, and a fetal calf serum culture medium is used for adding rat tail collagen for cell culture. By adopting the method provided by the invention, the epidermis and dermis structures are easy to distinguish, the hair follicle structure is clear and easy to separate, the cell passage time is shortened by about 13 hours, and the adherence is firmer. According to the method provided by the invention, the characteristics of the pig hair papilla cells can be kept within seven generations, and the purity is high, so that the pig hair papilla cells with high purity and relatively good passage consistency can be obtained in batches.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for culturing pig dermal papilla cells. Background technique [0002] The hair follicle is the most important accessory organ of the skin, and its development involves the interaction of ectoderm and mesoderm. The cyclical regrowth of the hair follicle (periodic cycle of anagen, catagen, and telogen phases) is characteristic of leading to the growth of new hairs and the shedding of old hairs. When the periodic regeneration function of hair follicles is abnormal, it can lead to hair follicle diseases such as alopecia, hirsutism and hair shaft disease. With the development of society, people pay more and more attention to the hair that affects life and work, and the research on the periodic development of hair follicles becomes more and more important. [0003] At present, the effective solution to the problem of hair loss is hair transplantation. Since hair tran...

AI Technical Summary

Problems solved by technology

Due to the special anatomical position of the dermal papilla, obvious biological characteristics, and difficulty in adhering to the wall, it is difficult to obtain high-purity dermal papilla cells, which restricts the research progress of hair follicle biology.

[0006] The traditional separation method of dermal papilla cells is by microdissection, but this method is labor-intensive, easy to cause operator fatigue, increases the probability of bacterial contamination, and the number of cells obtained is small, and the isolated dermal papilla cells are covered by the substrate. Membrane-coated, difficult to adhere to the wall and slow to migrate out

The commonly used enzymatic digestion method can obtain dermal papilla cells in batches, but due to the difference in hair follicle cycle development pattern and skin thickness in different parts, there are significant differences in the methods of extracting and culturing dermal papilla cells in different parts of different species, and Different culture conditions will affect the passage time of dermal papilla cells

The currently established dermal papilla cell culture method is mainly aimed at hair-using animals, and the dermal papilla of hair-using animals is very different from that of humans, which is not suitable for the research needs of human hair follicle science. However, the dermal papilla cell culture method for pigs It has not really been established, and it is impossible to provide high-purity dermal papilla cells in batches for research. Therefore, it is urgent to provide a method for extracting and culturing high-purity dermal papilla cells suitable for pig ear tissue, so as to further analyze and study human hair follicles. disease lays the groundwork

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