Preparation method of metal organic framework material for treating cerebral arterial thrombosis

A metal organic framework, ischemic stroke technology, applied in the field of biomedical materials, can solve problems such as inability to treat diseases, and achieve the effects of reducing nerve cell death rate, improving the microenvironment of the lesion, and reducing the infarct volume

Active Publication Date: 2021-07-02
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for preparing a metal-organic framework material for the treatment of ischemic stroke, so as to solve the problem in the prior art of researching and treating stroke in the pathological process of local brain tissue necrosis of ischemic stroke, The problem of not being able to treat the disease from the internal factors that cause the onset of stroke
[0009] In order to realize the above-mentioned preparation method of metal-organic framework materials for the treatment of ischemic stroke and solve the existing technology in the pathological process of local brain tissue necrosis of ischemic stroke to study the treatment of stroke, it cannot be caused by stroke The internal factor of morbidity sets out to treat the problem of disease, the present invention provides following technical scheme:

Method used

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  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis
  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis
  • Preparation method of metal organic framework material for treating cerebral arterial thrombosis

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Experimental program
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Embodiment 1

[0044] A method for preparing a metal-organic framework material for treating ischemic stroke, comprising the following steps:

[0045] Step 1, preparing a metal organic framework material;

[0046] Step 2, removing unreacted raw materials and solvent molecules in the metal organic framework material, the activated metal organic framework material;

[0047] Step 3, performing dopamine modification on the obtained metal organic framework material.

[0048] In this example, the preparation of the metal-organic framework material is specifically as follows:

[0049] Step 11, manganese chloride (MnCl 2 ), organic ligand hexaaminotriphenylene (HATP) are mixed in deionized water;

[0050] Step 12, add triethylamine dropwise to the liquid prepared in step 12, then heat and reflux at 80°C for 24h and then cool;

[0051] Step 13, centrifuging the supernatant of the liquid obtained in Step 12, and the obtained black powdery solid is the metal organic framework material.

[0052] In...

Embodiment 2

[0070] An application of a metal organic framework material prepared by the method for preparing a metal organic framework material as described above in the treatment of ischemic stroke, specifically:

[0071] 1. Polydopamine-modified organometallic frameworks (pDOPA-MOFs) were diluted with PBS to a concentration of 12.5 μg / ml in the treatment solution.

[0072] 2. After the C57 mice were anesthetized, the skin on the right side of the head was separated, and the bregma was used as the origin, and a hole was drilled with a cranial drill at a position 0.5 mm in the caudal direction and 1 cm parallel to the right side of the raphe;

[0073] 3. Separate the neck skin of the mouse, free the right external carotid artery, introduce the special thread plug for the obstruction model through the external carotid artery, and start counting down for 1 hour after reaching the designated position;

[0074] 4. Immediately after starting the timing, use the Hamilton needle to microinject t...

Embodiment 3

[0077] Such as figure 1 As shown, in this application, metal Mn and organic ligand HATP are self-assembled to form Mn-HATP metal-organic framework compound (Mn-MOF), and the surface of Mn-MOF is modified with dopamine to obtain pDOPA-MOFs.

[0078] Such as figure 2 As shown, in vitro, the superoxide dismutase (SOD) detection kit was used to detect the antioxidant enzyme activity of pDOPA-MOFs, that is, the ability to scavenge free radicals such as superoxide anion and hydrogen peroxide. Incubate pDOPA-MOFs with concentrations of 2.5, 5, 10, 12.5, 25, 50, 75, and 100 μg / ml with the detection test system, and measure the absorbance value corresponding to the specific absorption wavelength of the detection system (solution), reflecting the pDOPA-MOFs enzyme activity. The data proved that pDOPA-MOFs had SOD-like enzymatic activity in scavenging superoxide anion ( figure 2 ).

[0079] Among them, the principle of SOD enzyme detection (WST method) is: the reaction system of xa...

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Abstract

The invention discloses a preparation method of a metal organic framework material for treating cerebral arterial thrombosis. The preparation method comprises the following steps: step 1, preparing the metal organic framework material; step 2, removing unreacted raw materials and solvent molecules in the metal organic framework material, and activating the metal organic framework material; and step 3, carrying out dopamine modification on the obtained metal organic framework material. The metal organic framework material has superoxide dismutase (SOD), oxygen free radicals can be effectively converted into water and oxygen, meanwhile, the novel metal organic framework material can up-regulate the activity of endogenous antioxidant enzyme of nerve cells, and free radicals are further removed. The metal organic framework material is delivered in the stroke mouse in a lateral ventricle injection mode, the focus microenvironment is improved, the nerve cell death rate is reduced, the infarct volume is reduced, and the mouse postoperative behavioral function is effectively recovered.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of a metal organic framework material used for the treatment of ischemic stroke. Background technique [0002] Stroke is an acute cerebrovascular disease. According to the pathological characteristics, stroke is mainly divided into ischemic stroke and hemorrhagic stroke. Ischemic stroke accounts for more than 80% of the whole. Free radicals play an important role in the pathological process of local brain tissue necrosis in ischemic stroke. Due to the obstruction of the blood flow supply, the local nerve cells lack energy and oxygen, a large amount of calcium ions in the nerve cells flow in, and the mitochondrial function is damaged, causing excessive free radicals, such as superoxide anion (O2-), hydrogen peroxide ( H2O2), hydroxyl radicals (.OH) and reactive nitrogen (ONOO·), which will further damage nerve cells, causing cell apoptosis and ne...

Claims

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Application Information

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IPC IPC(8): C08G83/00A61P9/10
CPCC08G83/008A61P9/10
Inventor 王琳王征王健苏强飞汪洋夏克尔扎提肖哈拉提陈冲
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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