Sample diluent for ELISA detection kit and preparation method of sample diluent
A technology of sample diluent and detection kit, which is applied in the field of sample diluent and its preparation of ELISA detection kit, which can solve the problems of inability to effectively protect the sample to be tested and affect the accuracy and repeatability of the test results, and achieve the elimination of Effects of non-specific adsorption, improved detection sensitivity, improved accuracy and repeatability
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Embodiment 1
[0029] A sample diluent for the ELISA detection kit, calculated according to mass percentage, including 2% bovine serum albumin, 0.1% animal IgG, 5% maltose, 0.3% imidazole, 0.5% salt buffer, 0.05% Tween 20 and 0.03% Proclin300, the balance is purified water.
[0030] Animal IgG was obtained after purification of the negative serum ProteinAG of healthy mice; the salt buffer contained 12 g of anhydrous sodium dihydrogen phosphate and 9 g of sodium chloride per liter of salt buffer, and the solvent was purified water.
[0031] A preparation method for a sample diluent of an ELISA detection kit, comprising the steps of:
[0032] S1, weigh 12g of anhydrous sodium dihydrogen phosphate and 9g of sodium chloride into 500ml of purified water, stir until completely dissolved, and obtain salt buffer A;
[0033] S2, weigh 5g imidazole and 40g maltose, add in the salt buffer A in turn, stir for 3min, obtain solution B after dissolving;
[0034] S3, weigh 8g of bovine serum albumin and a...
Embodiment 2
[0037] A sample diluent for ELISA detection kit, calculated according to mass percentage, including 5% bovine serum albumin, 0.3% animal IgG, 8% arginine, 1.5% citric acid, 0.5% salt buffer, 0.1% Tween 20 and 0.18% Proclin300, the balance is purified water.
[0038] Animal IgG is obtained after purification of healthy rabbit negative serum ProteinAG; the salt buffer contains 12 g of anhydrous sodium dihydrogen phosphate and 9 g of sodium chloride per liter of salt buffer, and the solvent is purified water.
[0039] A preparation method for a sample diluent of an ELISA detection kit, comprising the steps of:
[0040] S1, weigh 12g of anhydrous sodium dihydrogen phosphate and 9g of sodium chloride into 700ml of purified water, stir until completely dissolved, and obtain salt buffer solution A;
[0041] S2, weigh 8g of citric acid and 60g of arginine, add them to the salt buffer A in turn, stir for 5min, and obtain solution B after dissolving;
[0042] S3, weigh 13g of rabbit s...
Embodiment 3
[0045] A sample diluent for ELISA detection kit, calculated according to mass percentage, including 4% bovine serum albumin, 0.2% animal IgG, 6% arginine, 1.0% imidazole, 0.5% salt buffer, 0.08% Tween 20 and 0.10% Proclin300, the balance is purified water.
[0046] Animal IgG was obtained after purification of healthy sheep negative serum ProteinAG; the salt buffer contained 12 g of anhydrous sodium dihydrogen phosphate and 9 g of sodium chloride per liter of salt buffer, and the solvent was purified water.
[0047] A preparation method for the sample diluent of ELISA detection kit, is characterized in that, comprises the steps:
[0048] S1, weigh 12g of anhydrous sodium dihydrogen phosphate and 9g of sodium chloride into 600ml of purified water, stir until completely dissolved, and obtain salt buffer A;
[0049]S2, weigh 6g of metal ion exchanger and 50g of protein stabilizer, add them into salt buffer A in sequence, stir for 4min, and obtain solution B after dissolving;
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