Method for assembling and annotating Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies

A genome and fur technology, applied in the field of biological information, can solve problems such as the unpublished genome of sheep, and achieve the effects of high integrity, good continuity and many repeating sequences

Pending Publication Date: 2021-06-22
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned patent does not disclose that it can be applied to the genome of sheep, and can construct a reference genome of guide black fur sheep at the chromosome level

Method used

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  • Method for assembling and annotating Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies
  • Method for assembling and annotating Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies
  • Method for assembling and annotating Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies

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Experimental program
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Embodiment 1

[0041] Example 1. A method for assembling and annotating the guide black fur genome based on three generations of PacBio and Hi-C technology

[0042] 1 Materials and methods

[0043] 1.1 Sample collection

[0044] One healthy adult ram (altitude >3500m) was selected in Guinan County, Guinan County, Hainan Tibetan Autonomous Prefecture, Qinghai Province, and 5ml of blood was collected from the jugular vein in the morning on an empty stomach and added to a blood collection tube with EDTA-K2 anticoagulant. , stored in -20°C refrigerator. After slaughtering and immediately dividing the heart, liver, lung, lung, spleen, rumen and muscle tissue, quickly rinse the blood on the surface of the tissue with pre-cooled normal saline, and cut into 0.5cm 2 Small pieces and stored in liquid nitrogen.

[0045] 1.2 Library Construction and Sequencing

[0046] The blood genome extraction kit (Tiangen Biochemical Technology Beijing Co., Ltd., Beijing) and the CTAB (cetyltrimethylammonium bro...

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Abstract

The invention belongs to the technical field of biological information, and particularly relates to a method for assembling and annotating a Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies. The method comprises the following steps: (1) collecting blood and tissue samples of Gueide black fur sheep; (2) constructing a genome library and a transcriptome library; (3) performing genome size and heterozygosis rate evaluation; (4) carrying out genome assembly, and carrying out error correction and evaluation by utilizing a transcription sequencing result; (5) carrying out Hi-C auxiliary assembly, error correction and evaluation; and (6) carrying out genome annotation and evaluation. The chromosome-level high-quality genome of the Guide black fur sheep is assembled, a foundation is laid for further research on wool pigment deposition and high-cold low-oxygen adaptive molecular mechanism of the Guide black fur sheep, and data support is provided for work of protection and utilization of excellent genetic resources, molecular breeding, genetic improvement and the like of the Guide black fur sheep.

Description

technical field [0001] The invention belongs to the technical field of biological information, and in particular relates to a method for assembling and annotating the guide black fur sheep genome based on three generations of PacBio and Hi-C technologies. Background technique [0002] Melanin is a kind of biological polymer, which widely exists in animals, plants and microorganisms. The color of animal hair, skin and eyes is determined by the relative quantity, nature and distribution of melanin. Melanin can inhibit the free radicals produced by ultraviolet radiation, absorb and reflect non-ion radiation in the form of particles, and can also act as a weak cation exchange polymer, which has the ability to bind a large number of metal ions or drugs, and can protect proteins from degradation. However, pigmentation is a complex trait controlled by multiple genes, and more than 300 loci have been identified among more than 150 genes affecting coat color in mammals. [0003] Gu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869G16B20/00C12N15/12
CPCC12Q1/6869G16B20/00C07K14/47C12Q2535/122C12Q2537/165C12Q2525/191
Inventor 卢曾奎刘建斌袁超
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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