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Molecular marker related to residual feed intake of sheep and application thereof

A molecular marker, feed intake technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems such as ME1 gene sequencing and analysis that have not yet been seen, and achieve low-cost operation methods, retention Accuracy, cost reduction effect

Inactive Publication Date: 2021-06-08
GANSU AGRI UNIV
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Problems solved by technology

[0005] At present, there is no report on the correlation between different genotypes and the remaining feed intake of sheep by sequencing and analyzing the ME1 gene

Method used

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  • Molecular marker related to residual feed intake of sheep and application thereof
  • Molecular marker related to residual feed intake of sheep and application thereof
  • Molecular marker related to residual feed intake of sheep and application thereof

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Embodiment Construction

[0039] The present invention will be further described in detail below with reference to the accompanying drawings and examples, and the embodiments are intended to be construed as limiting the scope of the invention.

[0040] (1) ME1 gene amplification

[0041] (1) primer design

[0042] Refer to the sheep ME1 gene DNA (Genbank Collection Number: NC_040259.1), using Oligo7.0 software design a pair of primers M-F and M-R, the primer sequence is as follows:

[0043] M-F: 5'-gaAcctagactggctattcgt-3 '(seq.id.no.2)

[0044] M-R: 5'-atcaagcagaactaccgacca-3 '(seq.id.no.3)

[0045] (2) amplification and sequencing of ME1 gene

[0046] The total volume of the PCR reaction system was 25 μL: a genomic DNA template 1 μL, 2 × PCR MASTER MIX 12.4 μL, upstream primer M-F 0.8 μL (10 μm), downstream primer M-R 0.8 μL (10 μm), and DDH 2 O 10 μL.

[0047] PCR amplification procedure: 94 ° C predetermitability 3min; 94 ° C degeneration 30s, 54.5 ° C annealing 30s, 72 ° C extends 30s, circulating 35 ...

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Abstract

The invention discloses a molecular marker related to residual feed intake of sheep and application thereof. Sheep ME1 gene is subjected to PCR amplification and sequence analysis, discovery shows that a C / T polymorphic site exists at the 453rd site of an amplified fragment, a KASPar primer pair is further used for detecting the corresponding polymorphic site and establishing a least squares model, genotypes and residual feed intake of 507 Hu sheep are subjected to correlation analysis, and the existence of the molecular marker related to the residual feed intake of the sheep in the amplified ME1 gene segment is determined. In the breeding practice, TT homozygous sheep can be selected into a core group, and the feed consumption and the breeding cost can be reduced by reducing the residual feed intake.

Description

Technical field [0001] The present invention relates to molecular marking auxiliary selection of novel meat sheep, and specifically, a single nucleotide polymorphism (SNP) site of ME1 gene and is used as a molecular marker of the remaining feed of sheep. Background technique [0002] In the production practice of meat sheep, the cost of forage material accounts for 65% -70% of the total cost, so improving feed efficiency is of great significance to economic and environmental protection. Residual feed intake (RFI) refers to the actual food and expectation of livestock and poultry and expectations to be calculated according to the requirements of production performance (body weight gain, milk production and egg production). The difference in feeding (KOCH, RM, ET Al., Efficiency of Feed Use in Beef Cattle.janim Sci.1963, 22 (2): 486-494). RFI reflects the difference in feed utilization efficiency caused by livestock and poultry itself by genetic background, which is independent of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 汪晓娟张德印王维民李发弟张小雪李冲袁律峰赵利明张煜坤
Owner GANSU AGRI UNIV
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