Complex plant extracts, compositions and methods of use for controlling the growth of Clado algae
A technology of plant extracts and compositions, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as secondary pollution and environmental degradation, and achieve easy operation and avoid violent Influence and improve the effect of the pool bottom
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Embodiment 1
[0033] A portion of Cladophora into 100mL containing natural seawater, and five species were added extract solution of different prescriptions, 1 Prescription: thyme extract 15%; Prescription 2: extract Perilla extract 15% + 3 Thyme %; prescription 3: thyme extract was 15% + 3% peppermint extract; prescription 4: thyme extract was 15% + 3% eucalyptus leaf extract; 5 prescription: thyme extract was 15%, perilla extract 3 %, peppermint extract 3%, 3% eucalyptus leaf extract, clove extract 1%. As used five types of co-solvents are square ethanol, 10% by volume, solubilizer poloxamer, the mass percentage is 3%, the rest was added at a concentration of 500ppm to 1000ppm and distilled water. Follow-up observations Cladophora growth conditions and changes.
[0034] The results showed that, at 1000ppm concentrations of five different prescription of plant extract are such that Cladophora discoloring, prescriptions 1 Cladophora fading, the upper suspension part is white, the lower yellow, ...
Embodiment 2
[0035] Example 2: a solution of different concentrations of plant extracts on the growth and survival of Cladophora.
[0036] Experiments with Cladophora algae samples were collected Qingdao Jimo aquaculture ponds. First, the initial sample was washed with water and then washed several times with water algae after sterilization to remove sediment and other debris to the surface of algae, and laboratory simulation pool containing natural seawater holding culture. All the experiments were in the experimental group 12:12 light: dark cycle cultured under light intensity of 100μmol. photon. m -2 . s -1 .
[0037]The proportion of each component in the composite plant extract is: 15% of the extract of the extract, 3% purple extract, 3% of the mint extract, 3% of the eucalyptus leaf extract, 1% lilac extract, free solvent absolute ethanol The percentage of volume is 10%, and the mass percentage of solubol Poopo Sham is 3%, and the rest is distilled water.
[0038] A portion of the diamel...
Embodiment 3
[0040] Example 3: Effect of plant extract solution on the growth and survival of rigidiacin at different temperatures
[0041] Experiments were collected and indoor cultivation in the production of rigidiacolic samples, see Example 2. A portion of the diamel was transferred into 100 ml of natural seawater, and the complex plant extract was added. According to the first part of the experimental results, the experimental concentration value was selected, the culture temperature was 20 ° C, 25 ° C, 30 ° C three gradients, the experimental cycle is 7 sky. During the experiment, a portion of the algae was taken under the microscope every day.
[0042] The results showed that according to the appearance of the diamel in the experiment, it was found that the rigidah under different temperature conditions changed, under 20 ° C and 25 ° C, the green algae gradually became yellow, and some The algae occurs in a white-changing phenomenon; the thiol in the case of 30 ° C is completely faded f...
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