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Hepatitis B virus adsorbent based on nanostructure as well as preparation method and application of hepatitis B virus adsorbent

A hepatitis B virus and nanostructure technology, applied in the field of biomedical adsorption materials, can solve the problems of difficult ligand surface size and geometry, affecting ligand recognition ability, high immunogenicity of antibodies, etc., to achieve reduced content, high Practical application value, efficient adsorption effect

Active Publication Date: 2021-05-28
WUHAN RUIFA MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this patent, antibodies are used as ligands, and the production of antibodies requires high costs and a long time. Antibodies will undergo irreversible denaturation after a certain period of time at room temperature or higher temperatures, and antibodies have high immunogenicity of
In addition, in this patent, the adsorption carrier is simply coupled to the ligand antibody, which makes it difficult to control the size and geometry of the ligand surface, which in turn affects the recognition ability of the ligand.

Method used

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  • Hepatitis B virus adsorbent based on nanostructure as well as preparation method and application of hepatitis B virus adsorbent

Examples

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preparation example Construction

[0029] The invention provides a method for preparing a nanostructure-based hepatitis B virus adsorbent, comprising the following steps:

[0030] S1. Synthesis of DNA nanomaterials;

[0031] S2. After recovering and purifying the DNA nanomaterial synthesized in step S1, mix it evenly with the aptamer, and obtain the DNA nanomaterial connected with the aptamer after incubation, and dissolve it in a buffer solution for use;

[0032] S3. Put the adsorption carrier in the incubation tube, then add the DNA nanomaterial connected with the aptamer dissolved in the buffer solution obtained in step S2, and react fully to obtain the hepatitis B virus adsorbent based on the nanostructure.

[0033] In step S1, the synthesis of the DNA nanomaterial specifically includes the following steps:

[0034] Dissolve a preset amount of DNA single-strand dry powder in TE buffer, then mix it according to the amount of the same substance, add it to TM buffer, and incubate at a preset temperature to ob...

Embodiment 1

[0042] This embodiment provides a method for preparing a nanostructure-based hepatitis B virus adsorbent, which specifically includes the following steps:

[0043] S1. Synthesis of DNA nanomaterials:

[0044] Dissolve eleven DNA single-strand dry powders in TE buffer (10mmol / L Tris-HCl, 1mmol / L EDTA, pH=8.0), and then mix the eleven DNA single-strands with the same amount of substances Add TM buffer (Tris-HCl of 20mmol / L, MgCl of 50mmol / L 2 , pH=8.0), incubate at 95°C for 5min, then cool down to 80°C and incubate for 3min, and then perform two stages of cooling (first cool down to 60°C at a rate of 1°C every 2min, then cool down to 60°C every 3min 1°C to 4°C), and then stored at room temperature for more than 10min to obtain a DNA nanomaterial with a DNA cube structure.

[0045] Among them, the DNA strand sequences of eleven DNA single strands are as follows:

[0046] Clip1-AB: 5′-TCGCT GAGTA HTCCT ATATG GTCAA CTGCT CHGCA AGTGT GGGCACGCAC ACHGT AGTAA TACCA GATGG AGTHC ACAAA...

Embodiment 2～6

[0070] Embodiments 2 to 6 respectively provide a preparation method of a nanostructure-based hepatitis B virus adsorbent. Compared with Example 1, the difference is that the type of DNA nanomaterial and adsorption carrier used has been changed. Each implementation The types of DNA nanomaterials and adsorption carriers corresponding to the examples are shown in Table 1.

[0071] The reaction parameters corresponding to Table 1 Embodiment 2~10

[0072] Example DNA nanomaterials Adsorption carrier Example 2 DNA octahedral cage spherical cellulose microspheres Example 3 DNA bipyramid spherical cellulose microspheres Example 4 DNA Cube silica gel Example 5 DNA Cube Polyvinyl alcohol microspheres Example 6 DNA Cube carbonized resin

[0073] Get the static blood sample from the hepatitis B patient, adopt the hepatitis B virus adsorbent prepared in Examples 1-6 respectively, according to the ratio of adsorbent: blood=1:5, t...

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Abstract

The invention provides a hepatitis B virus adsorbent based on a nanostructure as well as a preparation method and an application of the hepatitis B virus adsorbent. The method comprises the following steps: synthesizing a DNA nano-material with a specific structure, and connecting the DNA nano-material with an aptamer to obtain a DNA nano-material connected with the aptamer; and loading the DNA nano material connected with the aptamer on a specific adsorption carrier to obtain the hepatitis B virus adsorbent based on the nano structure. Through the mode, the DNA nano material can be used as an intermediate structure for connecting the aptamer and the adsorption carrier, so that complex operation required when the aptamer and the adsorption carrier are directly coupled can be avoided, and the preparation process is simplified; meanwhile, the adsorption efficiency of the adsorbent can be effectively improved while the aptamer and the adsorption carrier are stably connected, so that efficient adsorption of the hepatitis B surface antigen is realized, the content of the hepatitis B surface antigen in blood is greatly reduced, and the adsorbent has a relatively high practical application value.

Description

technical field [0001] The invention relates to the technical field of biomedical adsorption materials, in particular to a nanostructure-based hepatitis B virus adsorbent and its preparation method and application. Background technique [0002] Hepatitis B is a liver disease caused by hepatitis B virus (HBV) infection, including acute and chronic hepatitis. HBV surface antigen (HBsAg) seropositive is the main criterion for diagnosis. In clinical practice, clearing HBsAg is considered to be the most critical treatment endpoint of chronic hepatitis B, which is associated with improved clinical outcomes, prolonged survival, and reduced liver cirrhosis and hepatocytes. associated with the incidence of cancer. At present, the treatment methods for hepatitis B mainly focus on drug screening, drug administration and targeted therapy, etc., but the effects of these treatment options are limited, and the long-term treatment effect is not good. [0003] Based on the problems existin...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/28B01J20/30A61M1/36
CPCB01J20/24B01J20/28021B01J20/3071B01J20/3085A61M1/3679B01J2220/4856Y02A50/30
Inventor 王业富张磊郑豪
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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