Early screening method and kit for Crohn disease susceptibility genes

A susceptibility gene, Crohn's disease technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Active Publication Date: 2021-05-25
北京科力丹迪生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no very effective early detection method in clinical practice. Therefore, it is urgent to establish a highly sensitive, economical, and simple molecular genetic marker screening method. Through the screening of Crohn's disease susceptibility genes, early diagnosis and treatment can be used for early diagnosis of Crohn's disease. Patients bring good news, bringing personalized treatment and better prognosis to patients with Crohn's disease

Method used

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  • Early screening method and kit for Crohn disease susceptibility genes
  • Early screening method and kit for Crohn disease susceptibility genes
  • Early screening method and kit for Crohn disease susceptibility genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Feasibility analysis of SNP site screening of human Crohn's disease susceptibility-related genes

[0110] The inventors screened and evaluated loci related to the occurrence of Crohn's disease, which were verified in large-scale pathological control group clinical studies by searching NCBI's genome-wide association study (GWAS) at home and abroad. 18 single nucleotide polymorphism sites that are significantly associated with the risk of suffering from Crohn's disease are selected, and are independent of each other, and there is no linkage disequilibrium, so the site selection of the present invention has representativeness, independence and risk value Cumulative and can be used to assess an individual's risk of developing Crohn's disease.

[0111] The screened SNP sites are as follows:

[0112] rs72553867、rs4409764、rs3731772、rs17525495、rs13361189、rs9637876、rs10065172、rs2241880、rs6754677、rs2066844、rs2066847、rs7574865、rs1004819、rs1495465、rs11465788、rs4343、rs744166、rs1123...

Embodiment 2

[0113] Embodiment 2 system verification

[0114] System validation includes accuracy, specificity, sensitivity, precision, and comparison among personnel.

[0115] Accuracy verification scheme: 20 cases were detected at each site, compared with Sanger sequencing, the expected target was 95%.

[0116] Specificity Validation Protocol: Included in Accuracy, expected target 95%.

[0117] Sensitive verification scheme: using human genomic DNA positive samples as templates, the DNA contents of calibration samples were 1ng / μL, 5ng / μL, 10ng / μL, 50ng / μL, and 100ng / μL for sensitivity inspection.

[0118] The precision verification plan (including intra-batch, inter-batch, and personnel comparisons, not involving inter-instrument comparisons) has an expected target of 95%.

[0119] Intra-assay precision: The same batch of each sample was repeated 3 times to compare the intra-assay precision.

[0120] Inter-batch precision: The same operator tests the same sample in multiple batches to...

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Abstract

The invention provides an early screening method and a kit for Crohn disease susceptibility genes, and particularly relates to a method for screening out a combination of SNP sites of Crohn disease susceptibility related genes by considering the difference between gene spectrums of sick people and healthy people. Wide (high-throughput detection sites and high-throughput detection samples) screening and inspection are carried out on genetic markers related to the Crohn disease by using a nucleic acid mass spectrometer. The method disclosed by the invention is high in detection success rate, good in technical reproducibility and high in cost performance, can realize multi-gene detection of a single small sample, and meets maximum use of the small sample; the method has the technical advantages of high accuracy and high sensitivity, the detection result is stable, and the detection positive rate is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to an early screening method and kit for Crohn's disease susceptibility genes. Background technique [0002] Crohn's disease (CD) is an intestinal inflammatory disease of unknown cause, also known as Crohn's disease, Crohn's disease, etc. It can occur in any part of the gastrointestinal tract, and it may be related to infection, body fluid There is a certain relationship between immunity and cellular immunity. It is an autoimmune abnormality disease that occurs in people with some susceptibility systems (carrying susceptibility genes) under the stimulation of external environmental factors, and it is also a chronic inflammatory granulomatous disease of the gastrointestinal tract that involves multiple genes . A large number of signal pathway research evidences show that the related gene loci are closely related to the genetic susceptibility of CD and play an imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627C12Q2521/525
Inventor 徐力
Owner 北京科力丹迪生物医疗科技有限公司
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