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Preparation method and application of lipopolysaccharide composite nanoparticles

A polysaccharide composite and nanoparticle technology, applied in the field of biomedicine, can solve the problems of poor tissue penetration of PDT, and achieve the effect of improving antigen presentation efficiency, high feasibility and activating immune response.

Inactive Publication Date: 2021-05-25
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Different from PDT, PDT has poor tissue penetration, ultrasound can penetrate deep into the tissue, and target tumor tissue to mediate the cytotoxicity of ultrasound sensitizers

Method used

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  • Preparation method and application of lipopolysaccharide composite nanoparticles
  • Preparation method and application of lipopolysaccharide composite nanoparticles
  • Preparation method and application of lipopolysaccharide composite nanoparticles

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Embodiment 1

[0041]In the present embodiment, the preparation method of lipopolysaccharide composite nanoparticles comprises the following steps:

[0042] 1) Dissolve 100mg of polylactic-co-glycolic acid (PLGA) in 20mL of dichloromethane to obtain a mixed solution;

[0043] 2) Slowly inject oxygen into the liquid fluorocarbon (PFP) for 5 minutes to obtain the oxygen-carrying liquid fluorocarbon (PFP). Take 2 mL of oxygen-carrying liquid fluorocarbon (PFP), 5 mL of lipopolysaccharide (LPS) and indocyanine green (ICG ) 15mg was added into water, then ultrasonic treatment was performed for 0.5min, and then oxygen was passed into it for 5min to obtain an emulsion;

[0044] 3) Mix the mixed solution obtained in step 1) with the emulsion obtained in step 2), emulsify for 3 minutes using an ultrasonic vibrator, pour into 5 mL of PVA aqueous solution (1% w / v), and vibrate for 3 minutes, and then Add 10 mL of isopropanol aqueous solution (2% v / v), and stir with magnetic force for 4 hours to fully ...

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Abstract

The invention discloses a preparation method and an application of lipopolysaccharide composite nanoparticles, and belongs to the technical field of biomedicine. The lipopolysaccharide composite nanoparticle has a stable outer membrane, the outer membrane is prepared from a polylactic acid-glycolic acid copolymer (PLGA), and a mixed aqueous solution composed of liquid fluorocarbon (PFP), indocyanine green (ICG), lipopolysaccharide (LPS) and water is encapsulated in the nanoparticle; in the composite nanoparticles, the loading capacity of ICG is 10.61% + / -0.12%, and the loading capacity of lipopolysaccharide is 1.70%. According to the lipopolysaccharide composite nanoparticles disclosed by the invention, on the basis of PLGA nanoparticles, immunologic adjuvants including LPS, ICG, oxygen and an oxygen-carrying phase change material PFP are added; therefore, the stability and half-life period of the ICG in the circulation process are improved, the ICG can be combined with photoacoustic power to induce the immune reaction of the organism, and the ICG and the LPS jointly activate the immune system. The product has popularization and application values in the aspect of tumor immunotherapy.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of lipopolysaccharide composite nanoparticles. Background technique [0002] In the past decade, the integration of diagnosis and treatment has become a booming field of cancer treatment research, which has brought diagnosis and treatment closer together and made the treatment plan more individualized. Phototherapy (such as photothermal therapy (PTT) and photodynamic therapy (PDT)) has shown great advantages in emerging tumor treatments due to its less invasiveness, less side effects, and easy control. Sonodynamic therapy (SDT) is a new tumor treatment method developed on the basis of photodynamic therapy (PDT). Unlike PDT, PDT has poor tissue penetration, and ultrasound can penetrate deep into the tissue and target tumor tissue to mediate the cytotoxicity of ultrasound sensitizers. Photoacoustic combined therapy (Sono-Photodynamic thera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/34A61K49/22A61P35/00A61K39/39A61K39/00
CPCA61K41/0057A61K9/5153A61K49/22A61P35/00A61K39/39A61K39/0011A61K2039/55572Y02A50/30
Inventor 赵光宗常淑芳
Owner CHONGQING MEDICAL UNIVERSITY
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