Cloned weakening strain of chicken virus mycoplasma
A kind of Mycoplasma gallisepticum, weakening technology, applied in the direction of bacteria, etc., can solve the problem of weak virulence, poor immunogenicity and so on
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[0022] The cultivation and identification of the present invention are further described below:
[0023] 1. Common media formulations for cell-free culture
[0024] a.
[0025] Beef Heart Soup 1000ml
[0026] Niacinamide 30mg
[0027] Glucose 10g
[0028] Bannon Horse Serum 200ml
[0029] Phenol red 25mg
[0030] 25% Yeast Extract 40ml
[0031] pH 8.0
[0033] B
[0034] Beef Heart Digestive Soup 1000ml
[0035] Milk protein hydrolyzate 5g
[0036] NaCl 5g
[0037] 25% yeast extract 50-100ml
[0038] NAD(COI) 0.02g
[0039] Phenol red 0.01-0.02g
[0040] Arginine 0.1-0.2g
[0041] Eagles minimum needs vitamins (100×) 10ml in the culture medium
[0042] Pig Serum 150ml
[0043]Thallium acetate 0.25g
[0044] Penicillin 1000u / ml
[0045] PH7.2
[004...
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