Kit for detecting helicobacter pylori antibody in human urine by colloidal gold method and preparation method thereof

Abstract

The invention discloses a kit for detecting a helicobacter pylori antibody in human urine by a colloidal gold method and a preparation method thereof. The kit provided by the invention comprises a colloidal gold test strip for detecting the helicobacter pylori antibody based on a double-antigen sandwich method and a sample diluent. The colloidal gold pad is coated with a colloidal gold labeled Hp antigen A; a line T of the detection pad is coated with an Hp antigen B; and the Hp antigen A and the Hp antigen B are helicobacter pylori matching antigens. The sample diluent is prepared from: 70-80 mM of Tris-HCl, 0.5-1 g / 100 ml of Tween 20, 0.5-1 g / 100 ml of PVP-K30 (Polyvinyl Pyrrolidone K30), 0.1-0.5 g / 100 ml of SDS (Sodium Dodecyl Sulfate), 0.8-0.9 g / 100 ml of NaCl and 0.04-0.06 g / 100 ml of prolin300. The kit provided by the invention can be used for detecting a urine sample, and compared with serum detection and sampling in the prior art, the kit provided by the invention is more convenient, painless and noninvasive.

Description

technical field [0001] The invention relates to the detection of Helicobacter pylori urease antibody, in particular to a kit for detecting the Helicobacter pylori antibody in human urine by a colloidal gold method and a preparation method thereof. Background technique [0002] In 1983, Australian scholars discovered a kind of arc-curved or S-curved microaerophilic bacteria that parasitized on the surface of gastric mucosa and deep layer of gastric mucus. Helicobacter pylori, referred to as Hp bacteria. It has been confirmed that Helicobacter pylori is the culprit of peptic ulcer (stomach ulcer, duodenal ulcer) and chronic active gastritis. Studies have shown that more than 90% of duodenal ulcers and more than 80% of gastric ulcers are caused by Hp infection is caused by Helicobacter pylori, and more than 60% of patients with chronic gastritis have Hp infection. Hp infection → chronic superficial gastritis → atrophic gastritis → intestinal metaplasia or atypical hyperplasia...

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly the following methods for the detection of Helicobacter pylori in clinical practice: (1) Direct rapid urease test with gastroscope, biopsy specimens are affected by bacterial density, proton pump inhibitors, antibacterial drugs, etc., which may easily cause false negative results , and gastroscopy detection is painful for patients; (2) Gene detection (using PCR detection) is highly sensitive, simple, fast and highly specific, but PCR requires extremely high technology, and specimens or instruments are easy to be detected. Contamination caused erroneous results; (3)C 13 / 14 The breath test has the advantages of high accuracy and specific values, but it is expensive and requires fasting, and C 14 Belonging to radioactive isotopes, radioactive, not suitable for pregnant women and children