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Total homocysteine test card

A technology of homocysteine ​​and test cards, which is applied in the direction of measuring devices, microbial determination/inspection, biochemical instruments, etc., can solve the problems of reducing the concentration of hydrogen peroxide in products, poor customer experience, unreliable test results, etc.

Pending Publication Date: 2021-05-18
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our research found that in addition to reducing the bound HCY to generate free HCY, the dissociation agent also has the effect of reducing hydrogen peroxide, which leads to the excessive dissociation agent reducing the concentration of product hydrogen peroxide, making the detection results unreliable and difficult to analyze. low sensitivity
In addition, the dissociation rate of the dissociated HCY by the dissociation solution is greatly affected by temperature and time, and the sample needs to be pretreated at a specific temperature for a fixed time, which increases the trouble of user operation and deteriorates the customer experience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This embodiment provides a method for preparing a total homocysteine ​​test card, which is prepared according to the following method.

[0045] (1) Preparation of reducing layer:

[0046] Preparation of reduction treatment solution:

[0047] Tris Buffer (pH8.5) 50mmol / L Tris(2-hydroxyethyl)phosphine hydrochloride 0mmol / L serine 0.2g / L

[0048] Diffusion layer treatment: cut cotton fiber (Ahlstrom, model 601) into 100mm×200mm, soak in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, place it horizontally in an oven at 45°C and dry it for 2 hours, take it out for later use .

[0049] (2) Oxide layer preparation:

[0050] Preparation of oxide layer treatment solution:

[0051] Phosphate buffer (pH7.4) 20mmol / L Dithiobipyridine 0mmol / L Tween 20 2g / L

[0052] Retention layer treatment: Cut the polyethersulfone porous membrane (Sartorius, product number 15400) into 100mm×200mm...

Embodiment 2

[0065] This embodiment provides a method for preparing a total homocysteine ​​test card, which is prepared according to the following method.

[0066] (1) Preparation of reducing layer:

[0067] Preparation of reduction treatment solution:

[0068] Tris Buffer (pH8.5) 50mmol / L Tris(2-hydroxyethyl)phosphine hydrochloride 1.0mmol / L serine 0.2g / L

[0069] Diffusion layer treatment: cut cotton fiber (Ahlstrom, model 601) into 100mm×200mm, soak in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, place it horizontally in an oven at 45°C and dry it for 2 hours, take it out for later use .

[0070] (2) Oxide layer preparation:

[0071] Preparation of oxide layer treatment solution:

[0072] Phosphate buffer (pH7.4) 20mmol / L Dithiobipyridine 0mmol / L Tween 20 2g / L

[0073] Retention layer treatment: Cut the polyethersulfone porous membrane (Sartorius, product number 15400) into 100mm×200...

Embodiment 3

[0086] This embodiment provides a method for preparing a total homocysteine ​​test card, which is prepared according to the following method.

[0087] (1) Preparation of reducing layer:

[0088] Preparation of reduction treatment solution:

[0089] Tris Buffer (pH8.5) 50mmol / L Tris(2-hydroxyethyl)phosphine hydrochloride 0.75mmol / L serine 0.2g / L

[0090] Diffusion layer treatment: cut cotton fiber (Ahlstrom, model 601) into 100mm×200mm, soak in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, place it horizontally in an oven at 45°C and dry it for 2 hours, take it out for later use .

[0091] (2) Oxide layer preparation:

[0092] Preparation of oxide layer treatment solution:

[0093] Phosphate buffer (pH7.4) 20mmol / L Dithiobipyridine 1.0mmol / L Tween 20 2g / L

[0094] Retention layer treatment: Cut the polyethersulfone porous membrane (Sartorius, product number 15400) into 100mm×...

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Abstract

The invention provides a total homocysteine test card. The total homocysteine test card comprises an upper cover, a base and total homocysteine test paper, wherein a sample adding hole is formed in the upper cover; a detection hole is formed in the base; the total homocysteine test paper is composed of a filter layer, a reduction layer, a blood filter layer, an oxidation layer and a reaction layer which are sequentially stacked from top to bottom; the reduction layer is soaked in a reduction layer treatment solution containing a disulfide bond reducing agent and is dried; the oxidation layer is soaked in oxidation layer treatment solution containing disulfide and phosphate and is dried; and the reaction layer is soaked in a reaction layer treatment solution and is dried. Compared with the prior art, the total homocysteine test card disclosed by the invention does not need a sample pretreatment step of a user, can eliminate the influence of excessive dissociation agents on a hydrogen peroxide product, and can detect total homocysteine in a human blood or urine sample.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a test card for total homocysteine. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing 4-carbon α-amino acid, which is derived from the intermediate product of methionine and cysteine ​​metabolism in food, and it does not participate in protein synthesis. Methionine is combined with ATP under the catalysis of S-adenosylmethionine synthetase to form S-adenosylmethionine (SAM), and after SAM donates a methyl group under the action of methyltransferase, it becomes S - Adenosylhomocysteine ​​(SAH), which is hydrolyzed to form homocysteine ​​and adenosine. [0003] Under normal circumstances, homocysteine ​​is mainly metabolized through the remethylation pathway and the transsulfuration pathway, and can be released outside the cell. Remethylation pathway: under the action of methionine synthetase, vitamin B12 is used as coenzyme, N5-methyltetrahydrofolate ...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/34C12M1/12C12Q1/527C12Q1/28C12Q1/26
CPCC12Q1/527C12Q1/28C12Q1/26G01N2333/90203C12Q2326/96
Inventor 刘献文邹继华梁毅鲍双玲方亮贾江花邹炳德
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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