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Method and agent for preventing and treating cyst nematode disease of beet

A technology for cyst nematodes and nematode diseases, applied in the field of control methods and control agents for beet cyst nematode diseases, capable of solving problems such as high cost of control and treatment, toxic residues, and environmental pollution

Inactive Publication Date: 2021-05-11
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a control method and a control agent for beet cyst nematode disease, to solve the problem of high cost of control and treatment of beet cyst nematode disease, environmental pollution, and toxic residues. And other issues

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  • Method and agent for preventing and treating cyst nematode disease of beet
  • Method and agent for preventing and treating cyst nematode disease of beet

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Take 100 g of soil infested by sugar beet cyst nematodes (hereinafter referred to as nematodes) and crush it, put it into a beaker, add water to soak the soil for 2-3 cm, and filter it with a 100-mesh filter after a large number of eggs float on the water surface, and use clean water to filter the soil The eggs were washed into the petri dish, sterilized by sodium hypochlorite for 3 min, washed with sterile water, and incubated in an incubator. Draw 50 microliters of nematode liquid (about 200 strips) into a 2 mL centrifuge tube, then add 1×10 10 , 2×10 8 , 1×10 8 , 5×10 7 Put 1.5 mL of Rhodospirillum rubrum at a concentration of cfu / ml into an incubator at 25 degrees Celsius and 60% relative humidity. The blank control uses sterile water, and the positive control uses abamectin. Each treatment is 30 strains, and the treatment is repeated. 3 times. Put them into an incubator at 25 degrees Celsius and 60% relative humidity, and observe the survival of nematodes unde...

Embodiment 2

[0023] The difference between embodiment 2 and embodiment 1 is that the photosynthetic bacteria used in embodiment 2 is Rhodopseudomonas palustris. In embodiment 2, different concentrations of Rhodopseudomonas palustris process the cyst nematode mortality statistics at different times as figure 2 shown.

[0024] From figure 1 and figure 2 It can be seen that different photosynthetic bacteria (Rhodospirillum rubrum and Rhodopseudomonas palustris) have certain lethal effects on cyst nematodes. Different concentrations of photosynthetic bacteria also had a certain lethal effect on cyst nematodes, and the higher the concentration, the better the lethal effect. The fatality rate gradually increased with the action time. When the concentration of photosynthetic bacteria reaches 2×10 8 When the simultaneous action time of more than cfu / ml reached 96 hours, the lethality of the two photosynthetic bacteria to cyst nematodes reached more than 90%, and the corrected mortality rat...

Embodiment 3

[0027] A concentration of 2×10 was selected on the basis of indoor toxicity assays 8 cfu / ml of Rhodospirillum rubrum for pot experiments. The beet seedlings were sown indoors and transplanted into sterile soil pots with a concentration of 2×10 8 The photosynthetic bacteria of cfu / ml carries out soaking treatment, and each pot seedling plants 1 sugar beet, and the inoculation concentration of each plant after transplanting is 2×10 8Cfu / ml of photosynthetic bacteria 2ml, with sterile water as a control, 1 day after transplanting, each beet plant was inoculated with 300 second-instar cyst nematodes, and each treatment was inoculated with 30 seedlings, repeated 3 times, placed in a greenhouse at 25°C, and exposed to light 14h / d culture. After 30 days of treatment, the plants were pulled out, and the cysts in the soil of each treatment were separated and counted by floating method. The cyst reduction rate was calculated according to the following formula: relative cyst inhibit...

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Abstract

The invention discloses a method and agent for preventing and treating a cyst nematode disease of beet. According to the method, rhodospirillum rubrum is applied to prevention and treatment of the cyst nematode disease of beet. In specific application, a bacterial suspension with a concentration of 1-2 * 10<8> cfu / ml is prepared from rhodospirillum rubrum and water; beet seedlings are transplanted after two pairs of true leaves of the beet seedlings are grown, root irrigation treatment via the bacterial suspension is conducted on the seedlings one day before transplanting, 1 ml of the bacterial suspension is used for root irrigation of each seedling, and then 20 ml of rhodospirillum rubrum with a concentration of 0.5-1 * 10<7> cfu / ml is applied for each seedling after transplanting. The method and the agent can inhibit and kill beet cyst nematodes, so the yield of beet is increased; and since photosynthetic bacteria are easy to culture, non-toxic and environment-friendly, the method and the agent have the advantages of low cost, high benefit, no environmental pollution, no toxic residue and the like.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a control method and a control agent for sugar beet cyst nematode diseases. Background technique [0002] Sugar beet cyst nematodes belong to the order Ceratidae, Heterodermaceae, and the genus Heteroderma, and they are quarantine pests for entry into my country. The disease originated in Germany and occurs in more than 50 countries in Europe, Asia and America. It is one of the most important plant nematode diseases in the world. Beet cyst nematodes have a wide range of hosts and can harm more than 200 species of plants in 23 families and 95 genera, mainly Chenopodiaceae and Brassicaceae, which can cause 25-70% loss of sugar beet yield, or even crop failure. [0003] The control of the disease is very challenging due to the fact that sugar beet cyst nematodes can survive for many years in the protective cyst (Sharma, 1998). At present, scholars have...

Claims

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Application Information

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IPC IPC(8): A01N63/20A01N25/04A01P5/00A01G13/00
CPCA01G13/00A01N25/04A01N63/20
Inventor 王殿东熊鑫廖静静蔡兆明罗路云
Owner YANGTZE NORMAL UNIVERSITY
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