Application of tauroursodeoxycholic acid in treatment of neonatal necrotic enterocolitis
A technology for tauroursodeoxycholic acid and enterocolitis, which is applied in the field of medicine and can solve the problems of unclear preventive and therapeutic effects of tauroursodeoxycholic acid, achieve good protection, improve survival rate, and reduce apoptosis. Effect
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Embodiment 1
[0035] In this embodiment, as figure 1 In the modeling process shown, a 5-day-old C57BL / 6 neonatal mouse model of necrotizing enterocolitis was prepared by artificial feeding combined with hypoxia and cold stimulation. The experiment was divided into three groups: normal control group (10 rats), neonatal necrotizing enterocolitis model group (15 rats), and neonatal necrotizing enterocolitis model TUDCA intervention group (20 rats).
[0036] The specific experimental process is as follows:
[0037] Mice were artificially fed: Rat milk substitutes such as petag brand milk powder were used, the total energy was 840 kJ / kg / d, the feeding amount was 50 μl / g, and feeding was given at regular intervals of 4 hours.
[0038] Hypoxia and cold stimulation: hypoxia and cold stimulation were controlled within 1 hour after the meal. First place them in a hypoxic bottle containing 100% nitrogen for 60 seconds, then place them in a refrigerator at 4°C for 10 minutes, and cold-stimulate them ...
Embodiment 2
[0053] In this example, an in vitro experiment on the IEC-6 cell line was carried out to further verify the effect of TUDCA.
[0054] IEC-6 was grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, according to 1×10 6 Cells / mL concentration was inoculated into 6-well cell culture plate, 37°C, 5% CO 2 Cultured for 12 hours to allow the cells to fully adhere to the wall. LPS and TUDCA were prepared using normal saline. IEC-6 cells were treated with lps (50 μg / ml) for 6 hours, and TUDCA was added to the final concentration of 50 μM, 100 μM, and 200 μM, and then the cells were collected for subsequent research. Figure 8 Photographs for IEC-6 cell cultures, such as Figure 8 As shown in part B of , lps induces increased IEC-6 cell death, as Figure 8 As shown in part C, the intervention of TUDCA can improve the death of IEC-6 and promote the survival of cells. The results of the above in vitro experiments are as follows: Figures 9 to 11 shown...
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