COVID-19 antigen detection card as well as preparation method and application thereof

A COVID-19, antigen detection technology, applied in the field of nanomaterials and nanomedicine, can solve the problems of rapid diagnosis and screening of patients with unfavorable pneumonia, long time and cycle of viral nucleic acid detection, and high requirements for hardware equipment, and achieves stable and stable labeled products. The effect of wide detection range and high sensitivity

Active Publication Date: 2021-04-30
厦门奥德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Nucleic acid detection is currently the "gold standard" for the diagnosis of new coronavirus infection. However, virus nucleic acid detection has a series of problems such as long detection time and cycle, high environmental requirements, high technical force requirements, high hardware equipment requirements, and high price. Provincial / municipal CDCs and larger tertiary hospitals are not conducive to the rapid diagnosis and screening of pneumonia patients, and are not conducive to the prevention and control of the epidemic

Method used

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  • COVID-19 antigen detection card as well as preparation method and application thereof
  • COVID-19 antigen detection card as well as preparation method and application thereof
  • COVID-19 antigen detection card as well as preparation method and application thereof

Examples

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Comparison scheme
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Embodiment 1

[0063] The rare earth nanoprobe of this embodiment is sodium yttrium fluoride coated with sodium erbium fluoride with a core-shell structure, and has a particle size of 20nm to 30nm. Its composition is:

[0064] NaErF 4 @NaYF 4 , where NaErF 4 It is an all-erbium-doped core structure; NaYF 4 is the shell, @ means NaYF 4 Coated in NaErF 4 surface.

[0065] Further, the rare earth nanoprobe is stable in the ground state, and emits three emission fluorescences in the wavelength ranges of 500-550nm, 640-680nm and 1500-1600nm under the action of an excitation light source of 808nm. NaErF 4 @NaYF 4 Fluorescence spectra of rare earth nanoprobes excited at 808nm, such as figure 2 shown. NaErF 4 @NaYF 4 TEM images of rare earth nanoprobes, such as image 3 shown.

[0066] The method for preparing the rare earth nanoprobe of this embodiment comprises the following steps:

[0067] Step 1: Synthesis of erbium sodium fluoride core structure: In the container, add oleic acid ...

Embodiment 2

[0071] Such as figure 1 As shown, the COVID-19 antigen detection card of the present embodiment includes:

[0072] Substrate 1; as the substrate of the COVID-19 antigen detection card;

[0073] Coating film 2; arranged in the middle of the upper surface of the substrate 1;

[0074] Sample pad 3: overlapped at one end of the upper surface of the coating film 3

[0075] Absorbent paper 4; overlapped on the other end of the upper surface of the coating film 3;

[0076] Wherein, the sample pad 2 is sprayed with a microsphere thread 21, and the microsphere thread 21 is the COVID-19 monoclonal antibody 1 labeled with the rare earth nanoprobe provided in Example 1; the coating film 3 is provided with a detection line 31 and a quality control line 32, the detection line 31 is close to the sample pad 2, the detection line 31 and the quality control line 32 are parallel to each other, and the distance between them is 3-5mm; the detection line 31 is coated with COVID-19 monoclonal An...

Embodiment 3

[0087] The COVID-19 antigen detection kit of the present embodiment comprises:

[0088] COVID-19 Antigen Detection Kit includes Antigen Extraction Solution R1, which consists of 1% Triton X-100, 150mM Sodium Chloride, 1% Sodium Deoxycholate, 0.1% Sodium Lauryl Sulfate, Tris-Hcl (pH7.4).

[0089] COVID-19 antigen detection card: adopt the COVID-19 antigen detection card provided in Example 2;

[0090] ID card with calibration curve: The COVID-19 antigen detection card is used to measure different antigen concentration calibrators, with the antigen concentration as the abscissa and the fluorescence signal ratio as the ordinate, draw a standard curve, write and generate the corresponding QR code The information is stored in the ID card. The corresponding two-dimensional code information on the reagent card can be read and the corresponding antigen concentration can be measured by the dry fluorescent immunoassay analyzer.

[0091] The method for the quantitative detection of CO...

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Abstract

The invention discloses a COVID19 antigen detection card as well as a preparation method and application thereof, and belongs to the field of nano materials and nano medicine, an adopted rare earth nano probe is erbium fluoride sodium coated sodium yttrium fluoride with a core-shell structure, the particle size is 20nm-30nm, the composition of the rare earth nano probe is NaErF4@NaYF4, and NaErF4 is a full erbium doped core structure, NaYF4 is a shell layer, and @ represents that the NaYF4 is coated on the surface of the NaErF4. The nano-probe is a rare earth fluoride nano-material and has the advantages of low background, long luminescence life, strong fluorescence signal, high signal-to-noise ratio and the like, the probe is connected with a new crown monoclonal antibody through a covalent bond for marking, a marking product is stable, and the COVID19 antigen detection card has the characteristics of high sensitivity, high accuracy, rapid and simple detection and the like, and can be used for screening and detection of early-stage COVID19 suspected patient throat swabs, nasal swabs, nasopharyngeal swabs and saliva samples, so that clinical definite diagnosis can be quickly and specifically assisted.

Description

technical field [0001] The invention belongs to the field of nanomaterials and nanomedicine, in particular to a COVID-19 antigen detection card, its preparation method and application. Background technique [0002] Based on the current epidemiological investigation of the new coronavirus, the incubation period is generally 3-7 days, and the longest is no more than 14 days. The main manifestations of the infected person are fever, fatigue, and dry cough. Upper respiratory symptoms such as nasal congestion and runny nose are rare. About half of the patients developed dyspnea more than a week later, and severe cases rapidly progressed to acute respiratory distress syndrome, septic shock, metabolic acidosis that was difficult to correct, and coagulation dysfunction. It is worth noting that severe and critically ill patients may have moderate to low fever or even no obvious fever during the course of the disease. [0003] The new type of coronavirus is also known as severe acu...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577G01N33/558G01N33/58G01N33/533G01N33/531C09K11/02C09K11/85
CPCG01N33/56983G01N33/577G01N33/558G01N33/587G01N33/582G01N33/533G01N33/531C09K11/02C09K11/025C09K11/7773G01N2333/165G01N2469/10
Inventor 张云张肖刘宁宋良
Owner 厦门奥德生物科技有限公司
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