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Preparation method and application of composite microbial deodorization microbial agent

A technology of compound microorganisms and deodorant bacteria agent, which is applied in separation methods, biosynthesis, chemical instruments and methods, etc., can solve the problems of secondary pollution and high cost of deodorization technology, achieve recycling, enhance deodorization ability, cost reduction effect

Pending Publication Date: 2021-04-30
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high cost and easy secondary pollution in the current deodorization technology, the present invention provides a preparation method and application of a compound microbial deodorant agent

Method used

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  • Preparation method and application of composite microbial deodorization microbial agent

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specific Embodiment approach 1

[0013] Specific embodiment one: the preparation method of the compound microbial deodorant bacterium agent of this embodiment is carried out according to the following steps:

[0014] 1. Screen deodorizing microbial strains: Separation and purification, primary screening, and re-screening of chicken manure from chicken farms, leachate from landfills, or composted products from landfills, and the strains after re-screening are subjected to hydrogen sulfide and ammonia gas The mensuration of the removal rate of n strains is obtained the deodorizing bacterial strain with deodorizing function, and wherein the removal rate of hydrogen sulfide reaches more than 83% after 15 days, and the bacterial strain that the removal rate of ammonia reaches more than 78% is the deodorizing bacterial strain with deodorizing function Bacterial strains, n strains of deodorizing bacterial strains with deodorizing function include at least 1 strain of bacteria, 1 strain of yeast and 1 strain of fungi ...

specific Embodiment approach 2

[0021] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the separation and purification method in the step one is: take by weighing the chicken manure of 5g chicken farm, landfill leachate or waste landfill compost finished product put Dilute into 45mL sterile water, use selective medium and dilution plate separation method for concentration gradient of 10 -3- 10 -8 The samples were inoculated separately and cultured to obtain a single colony; a single colony was picked and streaked 2 to 5 times on a selective medium plate to obtain a pure culture. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0022] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the method of preliminary screening in the step one is: the bacterial strain obtained after separation and purification is inoculated in 50g chicken manure according to 10% inoculum size and carries out fermentation culture, then On the 5th, 10th, and 15th day, the odor level was evaluated by sensory assay, and a single strain with deodorizing function was obtained through preliminary screening. Other steps and parameters are the same as those in the first embodiment.

[0023] In this embodiment, preliminary screening is carried out one by one by sensory testing method, and the odor level is: 0-no odor; 1-very slight odor; 2-obvious odor; 3-strong odor; 4-strong odor; 5 - Very strong odor; the odor level of the strains in the initial screening was ≤2, and it was preliminarily determined to be a microbial strain with deodorizing function.

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Abstract

The invention relates to a preparation method and application of a deodorizing microbial agent, particularly to a preparation method and application of a composite microbial deodorizing microbial agent. According to the invention, the problems of high cost and easy generation of secondary pollution of the deodorization technology are solved. The method comprises the following steps: 1, screening deodorization microbial strains; 2, carrying out enlarged culture on a deodorizing compound microbial agent; 3, preparing a mixed microbial agent; and 4, inoculating the mixed microbial agent obtained in the step 3 into a molasses culture medium for culturing. The invention also discloses application of the composite microbial agent in deodorization. According to the invention, the composite microbial deodorizing microbial agent has the advantages of no toxicity, no residue, no corrosion, no irritant gas, no secondary pollution to the environment, simple preparation process, low cost, small dosage and quick response, can quickly remove odor in a short time and inhibit the generation of odor, and can be widely applied to deodorization of municipal solid waste landfills, municipal sewage treatment plants and the like.

Description

technical field [0001] The invention relates to a preparation method and application of a deodorant bacterial agent. Background technique [0002] At present, with the development of the national economy, the continuous improvement of people's living standards and the rapid development of urbanization, the output of urban domestic waste in our country is also showing a trend of rapid growth. According to statistics, the annual garbage dump in my country's cities is as high as about 6 billion tons, and the output of urban domestic garbage is increasing at an annual rate of 8%-10%. Among the more than 600 large and medium-sized cities in China, more than half of them have fallen into the siege of garbage. If these garbage cannot be disposed of in a timely and effective manner. Over time, it will inevitably bring incalculable losses to the ecological environment on which human beings rely for survival and development. Therefore, the disposal and utilization of municipal solid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B01D53/84B01D53/58B01D53/52
CPCC12N1/20C12N1/16C12N1/14B01D53/84B01D53/58B01D53/52B01D2251/95Y02P20/59Y02A50/20
Inventor 唐建唐恒军司马卫平王海玥梁超廖熠李志华董建威万珊
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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