A kind of composite biological decomposing agent and preparation method thereof

A technology of decomposing agent and compound enzyme preparation, which is applied in the field of organic waste treatment and resource utilization, can solve the problems that nutrients cannot be effectively degraded, absorbed, and cannot be used for crops, and achieves shortened decomposing time, fast heating, high temperature and so on. long lasting effect

Active Publication Date: 2022-06-03
辽宁振兴生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while many enterprises are responding to national policies, the organic materials used have not been thoroughly and effectively decomposed. The materials that have not been thoroughly decomposed also contain many unfavorable factors, including pathogenic bacteria, weed seeds, insect eggs, antibiotics, and heavy metals. , toxic and harmful gases, etc., and the nutrients cannot be effectively degraded and cannot be absorbed by crops. Although the organic fertilizer produced by such materials can improve the physical structure of the soil, the negative factors brought about are conceivable

Method used

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  • A kind of composite biological decomposing agent and preparation method thereof
  • A kind of composite biological decomposing agent and preparation method thereof
  • A kind of composite biological decomposing agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of composite biological decomposing agent one:

[0030] a. Preparation of thermophilic Clostridium papyri: (1) Seed culture: inoculate the colonies on the plate medium into the seed culture solution, and culture at 55~60℃ for 16~24h in a shaking table, and the rotation speed is 200~260rpm;

[0031] Among them, the seed culture solution is: 50g cellobiose solution, 1g tryptone, 0.5g yeast extract, 0.15g ammonium chloride, 0.5g urea, 0.2g tripotassium citrate, 0.15g citric acid monohydrate, 0.1g Potassium dihydrogen phosphate, 0.1g sodium chloride, make up 100g with water, adjust the pH value to 6.6-7.0; prepare 1.5kg according to the above ratio.

[0032] (2) Expanded culture: inoculate the seed culture solution into the expanded culture solution at 1.5% of the inoculum, and culture at 55-60° C. shaker for 16-24 hours at a speed of 200-260 rpm;

[0033] Among them, the expanded culture medium is 0.01g of formaldehyde, 50g of cellobiose solu...

Embodiment 2

[0038] Embodiment 2: the preparation two of composite biological decomposing agent:

[0039] a. Preparation of Clostridium papyri thermophilus: (1) seed culture and (2) expanded culture are the same as in Example 1.

[0040] (3) preparation of inoculum: adding 0.2kg of protective agent and 25kg of carrier adsorbent to the product cultivated in step (2), and fully dried to obtain powdery inoculum;

[0041] Wherein, the protective agent is soybean protein and whey powder with a weight ratio of 3:1, and the carrier adsorbent is Jiayi powder.

[0042] b. The thermophilic Clostridium papyrus inoculum prepared in step a and other materials are prepared according to the following parts by weight: 5 parts of thermophilic Clostridium papyri, 2 parts of Lactobacillus thermophilus, 20 parts The compound bacteria (weight ratio of Bacillus subtilis, Pseudomonas, and Bacillus pumilus is 8:1:1), 2 parts of compound enzyme preparation (weight ratio of acid protease, endo-cellulase, xylanase)...

Embodiment 3

[0044] Example 3: Preparation control of Clostridium papyrus thermophilus

[0045] Referring to the preparation method in Example 1, induction was performed without adding formaldehyde in the expanded culture medium (non-induced group). In the inoculum obtained under the same preparation conditions, the content of the thermophilic Clostridium papyri solution for induction without adding formaldehyde (non-induced group) was 3.5 billion / ml, and adding formaldehyde to induce (induction group) thermophilic decomposition The content of Clostridium papyrus liquid is as high as 8.2 billion / ml. The cellulose degradation experiments of reed straw by Clostridium papyrus thermophilus prepared by the two methods are now compared.

[0046] Experimental method: 1. 50% reed straw powder, 10% rice bran husk, 34% rice husk distiller's grains, 4% corn flour, 1% urea, 0.4% yeast powder, 0.3% potassium bicarbonate It was reconstituted into a solid medium and sterilized at 121°C for 30 min at hi...

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Abstract

The invention discloses a composite biological decomposing agent, which is composed of the following components in parts by weight: 2-10 parts of Clostridium papyrusii, 1-5 parts of thermophilic Lactobacillus, 10-30 parts of composite bacteria, 2-5 parts of compound enzyme preparation, 2-5 parts of compound actinomycetes, 5-10 parts of compound fungus, 40-70 parts of organic carrier, 0.001 part of tea tree essential oil; meanwhile, a preparation method of the compound biological decomposing agent is disclosed. The invention can efficiently, quickly and thoroughly decompose organic materials, has the characteristics of fast temperature rise and long duration of high temperature, can effectively kill pathogenic bacteria and insect eggs in the material, greatly shorten the decomposing time of one time, and reduce about one week. The material does not contain harmful bacteria, and the complex bacterial group in the product of the present invention is mostly of the genus Bacillus. This bacterial group can produce spores and is resistant to high temperature, so after the material is decomposed, the bacterial group can be greatly retained and play its role The role of soil and crops.

Description

technical field [0001] The invention relates to the technical field of organic waste treatment and resource utilization, in particular to a composite biological decomposing agent and a preparation method thereof. Background technique [0002] In recent years, with the excessive and unreasonable application of chemical fertilizers, the structure of the soil plough layer in my country has suffered serious damage, the soil is barren, the soil fertility is low, and various crop diseases, insect pests and soil-borne diseases have occurred frequently, resulting in crop yield and quality. affected on. From the perspective of soil fertilization, due to the increasing input of chemical fertilizers, while the environmental pollution caused by the increase is increasing, the input of organic fertilizers in traditional agriculture is gradually lost, resulting in a significant decline in soil fertility and soil organic matter content. The input amount of organic fertilizer in my country i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/20C05F17/80C12N1/20C12N1/14C12R1/145C12R1/225C12R1/01C12R1/645
CPCY02W30/40
Inventor 李建林薛万里张加明赵一
Owner 辽宁振兴生物科技发展有限公司
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