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Serum-free cell culture solution suitable for tumor stem cell enrichment and culture

A tumor stem cell and cell culture technology, applied in the field of serum-free cell culture medium, can solve the problems of inability to maintain the stemness of tumor stem cells and complex components

Pending Publication Date: 2021-04-09
CHENGDU ZHIA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its components are relatively complex, and each component is prone to antagonism. While promoting the proliferation of stem cells, it also promotes the osteogenic / adipogenic / chondrogenic differentiation of stem cells, resulting in the inability to maintain the stemness of tumor stem cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]A non-blood-free cell culture solution suitable for tumor stem cell enrichment and culture, characterized in that the components and their contents are as follows: 5 μL of 8 ng / ml growth factor, 8 ng / ml of growth factor, 4 μL, 15% BSA 1ml, 0.8mg / ml insulin 17 μL, 1000U / ml penicillin 100 μL, 1000 g / ml streptomycin 100 μL, NaHCO30.03mg, kh2PO4 0.005mg, NA2HPO4 0.005 mg, sulfate 0.0003 mg, phosphatidylcholine sodium salt 0.1 mg, composite vitamin 0.01 mg, plant polysaccharide 0.15 mg, composite amino acid 0.3 mg.

[0025]The plant polysaccharide is a wind-resistant polysaccharide; the composite vitamin is vitamin A, vitamin B2, vitamin C, vitamin D3, vitamin E, in mass ratio 1: 2: 1: 0.8: 1; the base medium is DMEM -F12; the DMEM-F12 culture solution is a F12 culture solution and DMEM culture solution mixed in a ratio of volume ratio 1: 3.

[0026]The growth factor is a platelet-derived growth factor-BB, platelet-derived growth factor AB, heparin binding epidermis (HB-β) and ...

Embodiment 2

[0029]A non-blood-free cell culture solution suitable for tumor stem cell enrichment and culture, characterized in that the components and their contents are as follows: 5 μl of base medium 50 mL, 9 ng / ml growth factor, 5 μl, 17% BSA 1.2ml, 0.9mg / ml insulin 19 μL, 1000U / ml penicillin 130 μL, 1000 g / ml streptomycin 120 μL, NaHCO30.033mg, kh2PO4 0.0055mg, NA2HPO4 0.0053 mg, sulfate 0.0004 mg, phosphatidylcholine sodium salt 0.12 mg, composite vitamin 0.015 mg, plant polysaccharide 0.17 mg, composite amino acid 0.33 mg.

[0030]The plant polysaccharide is a yellow poly sugar; the composite vitamin is vitamin A, vitamin B2, vitamin C, vitamin D3, vitamin E, in mass than 1: 2: 1.2: 0.9: 1.3 mixed; the base medium is DMEM -F12; the DMEM-F12 culture solution is a F12 culture solution and DMEM culture solution mixed in a ratio of volume ratio 1: 3.

[0031]The growth factor is a platelet-derived growth factor-BB, platelet-derived growth factor AB, heparin binding epidermis (HB-β) and insu...

Embodiment 3

[0034]A blood-free cell culture solution suitable for tumor stem cell enrichment and culture, characterized in that the components and their contents are as follows: 55 ml of base medium, 11 ng / ml growth factor 10 μl, 3.5 ng / ml deparagm tissue 6 μL, 20 % BSA1.5mL, 1 mg / ml insulin 21 μL, 1000U / ml penicillin 150 μL, 1000 g / ml streptomycin 130 μL, NaHCO30.035mg, KH2PO4 0.006mg, NA2HPO4 0.0055 mg, sulfate 0.0005 mg, phosphatidylcholine sodium salt 0.2 mg, 0.2 mg of plant polysaccharide, 0.35 mg of composite amino acid.

[0035]The plant polysaccharide is a polysaccharide; the composite vitamin is vitamin A, vitamin B2, vitamin C, vitamin D3, vitamin E according to mass ratio 1: 2: 1.5: 1: 1.5; the base medium is DMEM -F12; the DMEM-F12 culture solution is a F12 culture solution and DMEM culture solution mixed in a ratio of volume ratio 1: 3.

[0036]The growth factor is a platelet-derived growth factor-BB, platelet-derived growth factor AB, heparin binding epidermis (HB-β) and insul...

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PUM

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Abstract

The invention discloses a serum-free cell culture solution suitable for tumor stem cell enrichment and culture. The serum-free cell culture solution is characterized by comprising the following components: 45-65mL of a basic culture medium, 8-12 microliters of a growth factor with the concentration of 8-13ng / mL, 4-8 microliters of decellularized tissue with the concentration of 2-5ng / mL, 1-2mL of 15-25% BSA, 17-24 microliters of insulin with the concentration of 0.8-1.3mg / mL, 100-200 microliters of penicillin with the concentration of 1000u / mL, 100-150 microliters of streptomycin with the concentration of 1000g / mL, 0.03-0.04mg of NaHCO3, 0.005-0.007mg of KH2PO4, 0.005-0.006mg of Na2HPO4, 0.0003-0.0007mg of strontium sulfate, 0.1-0.2mg of phosphatidylcholine sodium salt, 0.01-0.03mg of compound vitamin, 0.15-0.25mg of plant polysaccharide and 0.3-0.4mg of compound amino acid. The invention further provides application of the serum-free cell culture solution suitable for tumor stem cell enrichment and culture. The serum-free cell culture solution suitable for tumor stem cell enrichment and culture, disclosed by the invention, is rich in nutrition, good in cell proliferation effect, excellent in stem retention effect and suitable for tumor stem cell enrichment and culture.

Description

Technical field[0001]The present invention relates to the field of cell biology, and more particularly to a blood-free cell culture medium suitable for tumor stem cell enrichment and culture.Background technique[0002]Tumors are common diseases and frequent diseases that seriously threaten human health. It is one of the main causes of death. Malignant tumors are becoming the number one killer threatening human life, and the annual prevalence of tumors is gradually increased. Tumor cells are affected by complex microenvironment, and have been gain genetic mutations during proliferation, leading to tumor cells having a variety of phenotytes and differentiation in tumor tissue. Tumor stem cells are common tumor cells that have self-renewal and self-replication in tumor tissues; with strong tolerance of chemotherapeutic drugs and radiation therapy, and can differentiate different phenotype tumor cells, reconstruction Heterogeneity of tumor tissue. Enrichment and culture of tumor stem cel...

Claims

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Application Information

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IPC IPC(8): C12N5/095
CPCC12N5/0695C12N2500/12C12N2500/20C12N2500/32C12N2500/38C12N2500/90C12N2501/10C12N2501/105C12N2501/135C12N2501/33C12N2501/90C12N2501/999
Inventor 向兴力
Owner CHENGDU ZHIA TECH CO LTD
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