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Lactobacillus casei for resisting helicobacter pylori and application thereof

A technology of Helicobacter pylori and Lactobacillus casei, applied in the field of microbial technology and microbial pharmacy, can solve the problems of gastric acid killing, low antibacterial titer, weak gastrointestinal colonization ability, etc. The effect of the application foreground

Active Publication Date: 2021-04-09
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most lactobacilli are isolated from feces, intestines or food, and are easily killed by gastric acid, with weak gastrointestinal colonization ability and low antibacterial potency. Therefore, further research and development have good gastric acid tolerance and antibacterial The ability of Lactobacillus to be used against Helicobacter pylori has important implications for the field of microbiology pharmaceuticals

Method used

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  • Lactobacillus casei for resisting helicobacter pylori and application thereof
  • Lactobacillus casei for resisting helicobacter pylori and application thereof
  • Lactobacillus casei for resisting helicobacter pylori and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening and Identification of Example 1 Lactobacillus casei HXL.casei 20-1

[0028] 1. Screening of Lactobacillus casei HXL.casei 20-1

[0029] 1.1 Sample source

[0030] The strains used in the present invention are collected from gastric mucosal tissue of volunteers undergoing gastroscopy.

[0031] 1.2 Configuration of medium

[0032] The medium used for sample isolation and strain screening was improved MRS solid medium, and the composition of the improved MRS solid medium was shown in Table 1; 1.5% agar in Table 1 was canceled to obtain an improved MRS liquid medium.

[0033] Table 1 MRS solid medium formula

[0034]

[0035] 1.3 Isolation and purification of strains

[0036] The obtained mucosal fluid was serially diluted 10 times with 0.9% normal saline, and spread on a modified MRS agar plate (MRS solid medium) containing 0.04% bromocresol purple, and cultured at 37°C for 48 hours. Pick the colonies with obvious yellow color circles from the single colo...

Embodiment 2

[0051] Example 2 Detection of Lactobacillus casei HXL.casei 20-1 Acid Resistance

[0052] The overall pH condition in the human gastric environment is strongly acidic. Therefore, the acid resistance of the strain is an important indicator to evaluate whether it can survive and colonize in the gastric acid environment. In order to simulate the gastric acid environment, the isolated Lactobacillus casei HXL.casei20-1 (hereinafter referred to as HXL.casei 20-1) and the Lactobacillus casei standard strain L.casei BNCC134415 (hereinafter referred to as the standard strain) were tested under different acidic pH environments. culture and comparative analysis.

[0053] Configure the modified MRS liquid medium with pH of 2.0, 3.0, and 4.0 respectively, activate HXL.casei 20-1 and the standard strain, and inoculate them into the medium with different pH, control the inoculation concentration at 2%, and at the same time use aseptic improvement MRS liquid medium was used as a control, cul...

Embodiment 3

[0056] Example 3 Lactobacillus casei HXL.casei 20-1 Bile Salt Resistance Determination

[0057] After being digested by the stomach, the bacteria will come to the small intestine, where there is a high concentration of bile salts, and the presence of bile salts will damage the cell membrane function of the bacteria. Therefore, testing the tolerance of bacteria to bile salts is also an important indicator for evaluating the survival efficiency of probiotics. In order to simulate the inhibitory effect of bile salts in the small intestine, the isolated HXL.casei 20-1 was separated from the standard strain in different Cultured under the environment of bile salt concentration and comparative analysis.

[0058] Prepare the modified MRS liquid medium with bile salt addition concentrations of 0.1%, 0.2%, 0.3%, and 0.4%, and inoculate HXL. The concentration is 2%, and the aseptic modified MRS liquid medium is used as the control at the same time, cultivated at 37°C, and samples are t...

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Abstract

The invention discloses lactobacillus casei for resisting helicobacter pylori and application thereof. The lactobacillus casei is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 21138. The lactobacillus casei strain grows well in a high-acid and high-cholate concentration environment, has high acid resistance and cholate resistance, has an obvious growth inhibition effect on helicobacter pylori, also has an obvious inhibition effect on helicobacter pylori adhering to human gastric mucosa GES-1 cells, and can inhibit in-vivo colonization of helicobacter pylori and relieve inflammation caused by pathogenic bacterium infection in animal experiments. The strain has wide application prospects in medicaments, functional products and health care products for preventing or treating gastric diseases caused by helicobacter pylori infection.

Description

technical field [0001] The invention belongs to the field of microbial technology and microbial pharmacy, relates to a newly screened microorganism, in particular to a Lactobacillus casei against Helicobacter pylori and its application. Background technique [0002] There are tens of billions of bacteria in the digestive system of the human body, which are composed of most normal bacteria and a small number of pathogenic bacteria and virus groups in a certain proportion. Any factor that causes flora imbalance may cause serious gastrointestinal diseases. In recent years, scholars have continuously discovered that the microecology of the digestive tract is closely related to various diseases such as digestive system diseases, cardiovascular and cerebrovascular diseases, and tumors. Many studies have found that most patients with chronic diseases such as obesity, constipation, colorectal cancer, and inflammatory bowel disease will experience symptoms of gastrointestinal microe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P1/04A23C9/123A23L2/38A23L2/84A23L33/135C12R1/245
CPCC12N1/20A23L2/382A23L2/84A23L33/135A23C9/1234A61K35/747A61P31/04A61P1/04A23V2002/00A23V2400/125A23V2200/3204Y02A50/30
Inventor 刘开云郭刚谭润卿李彦王宁刘宇
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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