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Swine oral inoculation vaccine slow-release adjuvant and preparation method and application thereof

A technology of vaccination and sustained release, applied in the direction of vaccines, veterinary vaccines, pharmaceutical formulations, etc., can solve the problems of unrealistic production and application, lack of immunity, disease in clean pigs, etc., to promote green breeding, and the synthesis process is simple and easy. The effect of line and operation is convenient

Pending Publication Date: 2021-04-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The eradication of pathogenic microorganisms requires simultaneous administration of large doses of antibiotics to pigs from various routes of administration, but this method is impractical for production applications
On the other hand, the pigs that have completely eliminated the pathogens lack corresponding immunity, and are very easy to be infected by the pigs carrying the pathogens that were mixed later, causing these clean pigs to get sick and causing major economic losses

Method used

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  • Swine oral inoculation vaccine slow-release adjuvant and preparation method and application thereof
  • Swine oral inoculation vaccine slow-release adjuvant and preparation method and application thereof
  • Swine oral inoculation vaccine slow-release adjuvant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Weigh 0.02g of poly 2-vinylpyridine (molecular weight is about 57kDa), dissolve it in 0.5mL of absolute ethanol to prepare a polymer ethanol solution with a mass concentration of 0.04g / mL. Disperse the inactivated Haemophilus parasuis vaccine in deionized water to prepare an aqueous antigen solution with a concentration of 1 mg / mL. Under the condition of continuous stirring, in the 0.5mL poly 2-vinylpyridine ethanol solution, dropwise add 5mL Haemophilus parasuis inactivated vaccine dispersion liquid with the flow rate of 1mL / min, after the two are stirred and mixed evenly, in 4 Incubate for 4 hours at °C. After the incubation is completed, filter the product mixture through a 0.45 μm filter membrane, collect the filtrate, purify and collect the polymer-antigen nanomicelles through a high-performance liquid chromatography system, and freeze-dry at -18°C for 12 hours to completely remove the solvent The poly 2-vinylpyridine vaccine slow-release adjuvant loaded with inac...

Embodiment 2

[0049] Weigh 0.01 g of poly-4-vinylpyridine (molecular weight is about 60 kDa), dissolve it in 0.5 mL of absolute ethanol to prepare a polymer ethanol solution with a mass concentration of 0.02 g / mL. Disperse the bivalent inactivated vaccine of Streptococcus suis in deionized water to prepare an aqueous antigen solution with a concentration of 1 mg / mL. In the case of continuous stirring, add 3 mL of Streptococcus suis bivalent inactivated vaccine dispersion solution dropwise to 0.5 mL of poly-4-vinylpyridine ethanol solution at a flow rate of 1 mL / min, stir and mix the two evenly, Incubate for 4 hours. After the incubation is completed, filter the product mixture through a 0.45 μm filter membrane, collect the filtrate, purify and collect the polymer-antigen nanomicelles through a high-performance liquid chromatography system, and freeze-dry at -18°C for 12 hours to completely remove the solvent to obtain the poly-4-vinylpyridine vaccine slow-release adjuvant loaded with bival...

Embodiment 3

[0052] Weigh 0.025g of poly-4-vinylpyridine (molecular weight is about 62kDa), dissolve it in 0.5mL of absolute ethanol to prepare a polymer ethanol solution with a mass concentration of 0.05g / mL. Disperse the inactivated Haemophilus parasuis vaccine in deionized water to prepare an aqueous antigen solution with a concentration of 1 mg / mL. In the case of continuous stirring, add 8 mL of Haemophilus parasuis inactivated vaccine dispersion solution dropwise at a flow rate of 0.5 mL / min to 0.5 mL of poly-4-vinylpyridine ethanol solution, stir and mix the two, and then To the mixture, 0.005 mL (4 mg) of peanut oil was added dropwise at a flow rate of 0.5 mg, stirred evenly, and incubated at 4° C. for 3 hours. After the incubation is completed, filter the product mixture through a 0.45 μm filter membrane, collect the filtrate, purify and collect the polymer-antigen nanomicelles through a high-performance liquid chromatography system, and freeze-dry at -18°C for 12 hours to complete...

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Abstract

The invention discloses a swine oral inoculation vaccine slow-release adjuvant and a preparation method and application thereof. The vaccine slow-release adjuvant, by mass percent, consists of 60%-80% of polymer and 20%-40% of antigen, or 45%-80% of polymer, 18%-27% of antigen and 2%-28% of immunologic adjuvant. The vaccine slow-release adjuvant can immunize a swine herb per os; after the swine is immunized per os, antigens start to gradually release after being carried to reach intestinal tracts, so that effective protection is provided for the entrapped antigens, the situation that the antigens are decomposed in the stomach to lose efficacy is prevented, controllable antigen delivery is realized, the exposure time in the intestinal tracts is extended, the effect of the antigens is fully exerted, and the problem of rapid glycolysis and clearing of the antigens in the digestive tracts is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a slow-release adjuvant for porcine oral vaccination and a preparation method and application thereof. Background technique [0002] In recent years, the amount of antibiotics has continued to increase significantly, mainly because antibiotics such as penicillin, tetracycline and chlortetracycline are widely used as feed additives in animal husbandry, and their main purpose is to increase livestock production rather than treat diseases. Antibiotic resistance occurs frequently around the world due to the overuse of antibiotics. The livestock farming industry is facing the same problem. For outbreaks of major infectious diseases in animals, the effectiveness of antibiotic prophylaxis or oral drug treatment is declining year by year. Taking the pig industry as an example, once a pig individual has clinical symptoms, not only the pigs showing sympt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/102A61K39/09A61P31/04
CPCA61K39/39A61K39/102A61K39/092A61P31/04A61K2039/55511A61K2039/542A61K2039/552Y02A50/30
Inventor 陈坤殷盼超张明鑫
Owner SOUTH CHINA UNIV OF TECH
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