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Bacterial colony counting method and device and computer storage medium

A colony counting and colony technology, which is applied in computer parts, computing, neural learning methods, etc., can solve the problems that the adherent colonies cannot be completely separated and the accuracy of the counting results is poor.

Pending Publication Date: 2021-04-06
北京陆桥技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing colony counting algorithm cannot completely separate the cohesive colonies, resulting in poor accuracy of the counting results

Method used

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  • Bacterial colony counting method and device and computer storage medium
  • Bacterial colony counting method and device and computer storage medium
  • Bacterial colony counting method and device and computer storage medium

Examples

Experimental program
Comparison scheme
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Embodiment 2

[0077] refer to figure 2 , shows a structural block diagram of the colony counting device of the second embodiment of the present application.

[0078] The colony counting device includes:

[0079] The first processing module 202 is used to perform noise reduction processing and edge feature extraction processing on the collected colony image, so as to obtain a colony binarized image including colony edge information and colony feature information in the colony image;

[0080] The second processing module 204 is configured to perform distance transformation processing on the colony binarized image, so as to convert the colony binarized image into a colony grayscale image, wherein the colony in the colony grayscale image The gray value of the edge of the colony indicated by the edge information is smaller than the gray value of the interior of the colony indicated by the characteristic information of the colony;

[0081] The third processing module 206 is configured to segme...

Embodiment 3

[0092] refer to image 3 , shows a schematic structural diagram of an electronic device according to Embodiment 3 of the present invention, and the specific embodiment of the present invention does not limit the specific implementation of the electronic device.

[0093] Such as image 3 As shown, the electronic device may include: a processor (processor) 302 , a communication interface (Communications Interface) 304 , a memory (memory) 306 , and a communication bus 308 .

[0094] in:

[0095] The processor 302 , the communication interface 304 , and the memory 306 communicate with each other through the communication bus 308 .

[0096] The communication interface 304 is used for communicating with other electronic devices or servers.

[0097] The processor 302 is configured to execute the program 310, specifically, may execute relevant steps in the above-mentioned comment method embodiment.

[0098] Specifically, the program 310 may include program codes including computer...

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Abstract

The embodiment of the invention provides a bacterial colony counting method and device and a computer storage medium. The bacterial colony counting method comprises the following steps: performing noise reduction processing and edge feature extraction processing on an acquired bacterial colony image to obtain a bacterial colony binary image containing bacterial colony edge information and bacterial colony feature information in the bacterial colony image; carrying out distance transformation processing on the bacterial colony binarization image to convert the bacterial colony binarization image into a bacterial colony gray level image, wherein the gray level value of the bacterial colony edge indicated by bacterial colony edge information in the bacterial colony gray level image is smaller than the gray level value of the interior of the bacterial colony indicated by bacterial colony feature information; according to the gray value in the bacterial colony gray image, carrying out bacterial colony segmentation on the bacterial colony gray image by using a watershed algorithm, and obtaining segmented bacterial colony information; carrying out connected domain analysis on the bacterial colony binarization image according to the segmented bacterial colony information; and determining a bacterial colony numerical value in the bacterial colony image according to a connected domain analysis result. The method is more accurate in counting.

Description

[0001] technology neighborhood [0002] The embodiments of the present application relate to the vicinity of biological equipment, and in particular to a colony counting method, device and computer storage medium. Background technique [0003] In the microbial inspection of food, medicine, cosmetics and feed, etc., a large number of colony counts are required when counting the results. Traditional colony counting mainly relies on visual observation, which is tedious, inefficient, and has problems such as the inability to save and trace the original data. Using digital image processing technology to count the number of colonies, on the one hand, can improve the work efficiency of experimenters, and on the other hand, can continuously optimize and improve the counting algorithm to achieve more accurate and fast results. However, the existing colony counting algorithm cannot completely separate the cohesive colonies, resulting in poor accuracy of the counting results. Contents...

Claims

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Application Information

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IPC IPC(8): G06T5/00G06T7/11G06T7/13G06N3/08G06N3/04G06K9/34
CPCG06T7/13G06T7/11G06N3/08G06T2207/20032G06T2207/30242G06V10/267G06N3/045G06T5/70
Inventor 何艳玲孙万东王杰伟刘丰瑞
Owner 北京陆桥技术股份有限公司
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