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A kind of preparation method and application of medical carbonate nanometer material

A technology of nanomaterials and carbonates, which is applied in the field of biomedical nanomaterials, can solve the problems that limit the development of nanomaterials, achieve the effect of inducing apoptosis and necrosis, and stimulating oxidative stress response

Active Publication Date: 2022-03-15
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic nanomaterials have the advantages of simple synthesis, large-scale production, and easy quality control, but the safety and health factors in clinical transformation limit the further development of nanomaterials, especially the in vivo degradation of inorganic nanomaterials is an urgent problem to be solved

Method used

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  • A kind of preparation method and application of medical carbonate nanometer material
  • A kind of preparation method and application of medical carbonate nanometer material
  • A kind of preparation method and application of medical carbonate nanometer material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Synthesis and characterization of embodiment 1 manganese carbonate nanoparticles

[0074] (1) Manganese chloride tetrahydrate and indocyanine green were mixed and stirred for 3 hours according to the mass ratio of 50:1-50:3;

[0075] (2) Place the material (Mn-ICG) gained in step (1) in ammonium bicarbonate (NH 3 HCO 3 ) in an environment of decomposition and diffusion, react at room temperature (30°C), and the reaction time can be controlled within 1-4h;

[0076] (3) The material obtained in step (2) is centrifuged at 5000rpm for 10min, and the precipitate is taken;

[0077] (4) ultrasonically disperse the precipitate obtained in step (3) in 20 mL of absolute ethanol, centrifuge and wash at 5000 rpm for 10 min, repeat this step 3 times, and obtain the precipitate;

[0078] (5) Disperse the precipitate obtained in step (4) in 50 mL of absolute ethanol, and add polyallylamine hydrochloride (PAH, molecular formula (C 3 h 7 N) n xHCl, the average molecular weight is ...

Embodiment 2

[0084] Example 2MnCO 3 -ICG nanomaterials for cell experiments

[0085] Taking murine breast cancer 4T1 cells as a research model, the MnCO prepared in Example 1 was further explored. 3 - Research on ICG nanomaterials for combination therapy of tumors. At the same time, human malignant glioma U87MG cells and human liver cancer HepG2 cells were used to verify MnCO 3 -Therapeutic effect of ICG nanomaterials on tumor cells.

[0086] (1) Cell uptake test

[0087] Seed 4T1 cells on glass bottom cell culture dish (confocal dish) Medium (10 5 cells), cultured for 24h, after the cells adhered to the wall, mixed with MnCO 3 -ICG medium ([Mn]: 5μg / mL) was co-incubated for different times (2, 4, 8, 12 and 24h), washed three times with PBS, stained with Hoechst33342 (5μg / mL) for nuclei, washed three times, supplemented For fresh culture medium, use Olympus FV1200 laser confocal microscope to detect the fluorescence of ICG to judge the uptake of materials by cells. Such as Figur...

Embodiment 3

[0108] Example 3MnCO 3 -ICG nanomaterials used in animal experiments

[0109] Experimental animal model: female BALB / c mice (body weight 16-18 g) were purchased from the Shanghai Slack Experimental Animal Center by the Experimental Animal Center of Xiamen University.

[0110] Tumor model: by subcutaneous injection of 100 μL of 4T1 cells (2×10 6 ) in the right hind leg of the experimental mice, inoculated subcutaneously with 4T1 tumors.

[0111] All animal experiments were in accordance with the animal protection regulations approved by the Experimental Animal Use Committee and Experimental Animal Ethics Committee of Xiamen University.

[0112] (1)MnCO 3 -Study on the distribution and metabolic behavior of ICG nanomaterials in vivo

[0113] A. Female BALB / c mice bearing 4T1 tumors when the tumor reaches 80-100mm 3 Intravenous injection of MnCO 3 - ICG nanomaterials ([Mn]: 2mg / kg) (n=3), at different time points (0.5, 1, 2, 4, 8, 12, 24, 48h) after drug injection, blood sa...

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Abstract

The invention discloses a preparation method and application of a medical carbonate nanomaterial, which comprises mixing divalent metal ions with medical organic functional molecules capable of coordinating with the divalent metal ions, and placing them in carbonic acid at room temperature. The reaction is carried out in an environment where ammonium hydrogen disperses and diffuses, and then reacted with polyallylamine hydrochloride. The divalent metal ion is Mn(II) or Ca(II), and the medical organic functional molecule is indocyanine green or Dexamethasone Sodium Phosphate.

Description

technical field [0001] The invention belongs to the technical field of biomedical nanomaterials, and in particular relates to a preparation method and application of medical carbonate nanomaterials. Background technique [0002] Cancer is still one of the important causes of global threats to human health, and cancer treatment remains a daunting challenge. At present, traditional clinical treatment methods commonly used include surgery, radiotherapy, chemotherapy, etc. These methods can cure local tumors and prolong the survival of patients, but they cannot inhibit tumor recurrence and metastasis. The combination of nanotechnology and modern biology and medicine provides more opportunities for the treatment of tumors. Inorganic nanomaterials have the advantages of simple synthesis, large-scale production, and easy quality control, but the safety and health factors in clinical transformation limit the further development of nanomaterials, especially the in vivo degradation o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/08A61K31/573A61K41/00A61K47/32A61P35/00B82Y5/00
CPCA61K49/0002A61K31/573A61K49/0034A61K49/08A61K41/0052A61K47/32A61P35/00B82Y5/00A61K2300/00
Inventor 陈洪敏冯宇硕孙文静
Owner XIAMEN UNIV
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