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Tissue adhesive as well as preparation method and application thereof

A tissue adhesive and cross-linking agent technology, used in applications, surgical adhesives, pharmaceutical formulations, etc., can solve the problems that cannot fully meet clinical needs, poor mechanical properties, and lack of cell affinity and tissue affinity for super-strong hydrogels. Adhesion and other issues, to achieve the effect of good wide applicability, good biocompatibility, and high bonding strength

Active Publication Date: 2021-04-02
CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional hydrogels have poor mechanical properties due to their high water content; general super-strong hydrogels lack cell affinity and tissue adhesion, and cannot fully meet clinical needs.

Method used

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  • Tissue adhesive as well as preparation method and application thereof
  • Tissue adhesive as well as preparation method and application thereof
  • Tissue adhesive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 2 g of gelatin (bovine source) was dissolved in 20 mL of deionized water at 60° C. to obtain a 10 wt / vol % gelatin solution. After adjusting the pH value to 8.5 with sodium hydroxide solution, use a syringe to drop 0.2 g / mL tannic acid aqueous solution into the gelatin solution (a total of 1 mL of tannic acid aqueous solution is used), and wait until it is completely dissolved and mixed evenly ( The time was controlled at 0.5 h), and the pH of the solution was adjusted to 7.1 with hydrochloric acid solution, which was recorded as solution I.

[0064] Dissolve 0.04 g of genipin in 1 mL of 70% ethanol solution at room temperature. Subsequently, solution I and genipin solution (volume ratio 1:1) were poured into a double-syringe syringe, extruded, at room temperature (25°C), in situ cross-linking reaction, reacted for 10 minutes, and obtained a Strong tissue adhesive.

[0065] Use a double-syringe syringe to extrude solution I and genipin solution (volume ratio 1:1) onto...

Embodiment 2

[0069] 1.5 g of gelatin (from fish) was dissolved in 20 mL of deionized water at 40° C. to obtain a 7.5 wt / vol % gelatin solution. After adjusting the pH value to 8.1 with sodium hydroxide solution, use a syringe to add 0.5 g / mL gallic acid aqueous solution dropwise into the gelatin solution (a total of 1 mL of gallic acid aqueous solution is used), and wait until it is completely dissolved and mixed evenly (time control At 0.75 h), the pH of the solution was adjusted to 7.1 with hydrochloric acid solution, which was designated as solution I.

[0070] Dissolve 0.08 g of genipin in 2 mL of 75% ethanol solution at room temperature. Subsequently, solution I and genipin solution (volume ratio: 3:1) were poured into a double-syringe syringe, extruded, at room temperature (20°C), in situ cross-linking reaction, reacted for 15 minutes, and obtained a high-strength adhesive Strong tissue adhesive.

[0071] Extrude solution I and genipin solution (volume ratio: 3:1) using a double-sy...

Embodiment 3

[0075] 1.5 g of gelatin (porcine origin) was dissolved in 30 mL of deionized water at 40° C. to obtain a 5 wt / vol % gelatin solution. After adjusting the pH value to 8.8 with sodium hydroxide solution, use a syringe to add 0.8 g / mL tannic acid aqueous solution dropwise to the gelatin solution (a total of 1 mL of tannic acid aqueous solution is used), and adjust the pH of the solution to 7.1 with hydrochloric acid solution , denoted as solution I.

[0076] Dissolve 0.1 g of genipin in 1 mL of 75% ethanol solution at room temperature. Subsequently, solution I and genipin solution (volume ratio 1.5:1) were poured into a double-syringe syringe, extruded, at room temperature (30°C), in situ cross-linking reaction, reacted for 20 minutes, and obtained a strong adhesive Strong tissue adhesive.

[0077] Extrude solution I and genipin solution (volume ratio: 1.5:1) with a double-syringe syringe onto the surface of pigskin, rabbit heart, aminated glass, etc. At the lap joint for 10 m...

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Abstract

The invention discloses a tissue adhesive as well as a preparation method and application thereof. According to the tissue adhesive, when the tissue adhesive adheres to the surface of pigskin for lessthan 8h, the adhesion strength is smaller than or equal to 55kPa, and when the surface adhesion time is more than or equal to 8h, the adhesion strength I greater than 55kPa. Due to the fact that thetissue adhesive contains amino, phenolic hydroxyl and a natural cross-linking agent, the tissue adhesive can form non-covalent and covalent bonds with groups such as the amino and the sulfydryl on thesurface of tissue, and the tissue adhesive has high adhesion strength through the synergistic effect of multiple action bonds. The preparation method of the tissue adhesive is simple in process and low in raw material cost, and the tissue adhesive is a natural source material. The tissue adhesive has good biocompatibility, is high in adhesive strength under physiological conditions, is simple, convenient and safe to use, and has a good application prospect in tissue adhesion repair.

Description

technical field [0001] The application belongs to the field of hydrogel preparation, and in particular relates to a tissue adhesive with high-strength adhesion performance and its preparation method and application. Background technique [0002] Due to reasons such as trauma or surgery, millions of patients in the world need to suture their wounds every year. Currently, sutures and rivets are used clinically to suture wounds. Although this method sutures firmly so that the wound is not easy to crack, it is widely used due to the inconvenience of operation, susceptibility to infection, scars after healing, affecting the appearance and increasing the pain of patients. Therefore, a tissue adhesive with high adhesion performance has been developed. very necessary. Adhesive hydrogels with high water content and tissue-like structure are among the most important biomaterials used as surgical sealants and wound dressings. They show superior wound closure and tissue regeneration ...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/104A61L24/0031A61L24/001A61L2400/06
Inventor 徐婷王荣张永和
Owner CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI
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