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Tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as preparation method and application thereof

A technology of compound microorganisms and tobacco bacterial wilt, which is applied in the field of anti-tobacco bacterial wilt growth-promoting compound microbial effervescent tablets and its preparation, can solve the problems of high transportation requirements and short bacterial viability maintenance time, and improve the survival rate , prolong the validity period of use, and prevent the effect of cracking

Inactive Publication Date: 2021-04-02
ZUNYI MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of them are compound liquid bacterial agents, which have problems such as high transportation requirements and short maintenance time of bacterial strains.

Method used

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  • Tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as preparation method and application thereof
  • Tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as preparation method and application thereof
  • Tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0033] The preparation method of the above-mentioned anti-tobacco bacterial wilt growth-promoting compound microbial effervescent tablet comprises the following steps:

[0034] Step 1, the preparation of bacterial powder

[0035] Inoculate Bacillus Velez and Bacillus subtilis respectively in the liquid fermentation medium under aseptic conditions, culture at 20-38°C for 24-72 hours, then ferment the fermentation liquid of Bacillus Velez and Bacillus subtilis The liquids were centrifuged separately, and the bacterial liquids in the lower layer were removed respectively, and set aside;

[0036] Step 2, preparation of composite functional bacterial liquid

[0037] Get the bacterium liquid of Bacillus subtilis and Bacillus Veles in step 1 and mix it after being 1:0.2-5 by weight, for subsequent use;

[0038] Step 3, preparation of white and extract

[0039] Take white and tubers, homogenate, soak in distilled water for 1h, heat to 80°C, extract for 45min, repeat 3 times, filter...

Embodiment 1

[0046] An anti-tobacco bacterial wilt growth-promoting compound microbial effervescent tablet, comprising the following raw materials in parts by weight: 10 parts of bacterial powder, 12 parts of tartaric acid, 40 parts of sodium bicarbonate, 12 parts of polyethylene glycol, 9 parts of lactose, microcrystalline 20 parts of cellulose, 1 part of magnesium stearate, 8 parts of white and extract, 3 parts of trehalose. The preparation method refers to the above content.

Embodiment 2

[0048] An anti-tobacco bacterial wilt growth-promoting compound microbial effervescent tablet, comprising the following raw materials in parts by weight: 15 parts of bacterial powder, 10 parts of tartaric acid, 40 parts of sodium bicarbonate, 10 parts of polyethylene glycol, 10 parts of lactose, microcrystalline 20 parts of cellulose, 1 part of magnesium stearate, 10 parts of white and extract, 5 parts of trehalose. The preparation method refers to the above content.

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Abstract

The invention discloses a tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as a preparation method and application thereof. The compound microorganism effervescent tablet is prepared from the following raw materials in parts by weight: 5-20 parts of bacterial powder, 5-20 parts of tartaric acid, 10-50 parts of sodium bicarbonate, 5-30 partsof polyethylene glycol, 2-20 parts of lactose, 10-40 parts of microcrystalline cellulose, 1-10 parts of magnesium stearate, 1-10 parts of bletilla striata extract and 3-8 parts of trehalose. Accordingto the invention, the compound microbial effervescent tablet prepared from the tobacco growth-promoting strain bacillus velezensis and the tobacco bacterial wilt antagonistic strain bacillus subtiliscan effectively inhibit tobacco bacterial wilt caused by ralstonia solanacearum. The bletilla striata extract is added, so that rapid forming of the effervescent tablet can be effectively promoted, the activity of the strains is kept, and the validity period of the effervescent tablet is prolonged. By adjusting the composition and structure of soil and rhizosphere microorganisms, the content of beneficial microorganisms is increased, pathogenic bacteria are effectively inhibited, and the purposes of promoting tobacco growth and effectively resisting bacterial wilt are achieved.

Description

technical field [0001] The technical field of tobacco growth of the present invention specifically relates to a tobacco bacterial wilt disease growth-promoting compound microbial effervescent tablet and its preparation method and application. Background technique [0002] Microbial preparations are often used in the prevention and treatment of tobacco bacterial wilt, which can promote the growth of tobacco seedlings and reduce the incidence of bacterial wilt. At present, most of them are compound liquid bacterial agents, which have problems such as high transportation requirements and short maintenance time of bacterial strains. [0003] Effervescent tablet is a new type of agricultural production product, which refers to the live bacteria product made by fermenting target microorganisms and effective bacteria, which can be used for seedling cultivation and root irrigation, and has the functions of maintaining the balance of plant root microorganisms, promoting plant growth,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N25/34A01N25/10A01N25/22A01P3/00
CPCA01N25/10A01N25/22A01N25/34A01N63/22
Inventor 贾启陈集双刘明宏彭玉龙王小彦陈忠文
Owner ZUNYI MEDICAL UNIVERSITY
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