Fermentation medium for preparing hydroxyl-containing carboxylic acid and salt thereof by utilizing bacillus coagulans and fermentation method

Abstract

The invention provides a fermentation medium for preparing hydroxyl-containing carboxylic acid and salt thereof by utilizing bacillus coagulans and a fermentation method. The fermentation medium contains an organic nitrogen source and an inorganic nitrogen source, and the mass ratio of the organic nitrogen source to the inorganic nitrogen source is 1:(1-6), preferably 1:(1.3-2.5). According to thefermentation medium provided by the invention, the OD value of the bacterial liquid is effectively controlled, meanwhile, the high conversion rate is realized by controlling the concentration of thecarbon source, the balance between the high OD value and the high conversion rate is achieved, and the yield of a fermentation product is not inhibited, so that the hydroxyl-containing carboxylic acidand the salt thereof prepared by utilizing the fermentation medium provided by the invention are high in production speed and high in conversion rate; besides, the finally obtained fermentation liquor is high in product concentration and high in optical purity.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and relates to a fermentation medium and a fermentation method for preparing hydroxycarboxylic acids and salts thereof by using bacillus coagulans. Background technique [0002] Lactic acid (Lactic Acid), also known as 2-hydroxypropionic acid, molecular formula is C 3 h 6 o 3 , it is a carboxylic acid containing hydroxyl, which has many uses. Lactic acid and its salts are used in food, medicine, chemical industry and other fields. [0003] Among them, sodium lactate is used in food preservation, moisturizing, flavoring and pharmaceutical raw materials, and can also be used as medicine to relieve acidosis caused by diarrhea, dehydration, diabetes, nephritis, etc. Plasticizer, antifreeze, humectant, substitute of glycerin, corrosion inhibitor of alcohol antifreeze; Potassium lactate is used as flavoring agent, emulsifier, hygroscopic agent, pH regulator, flavoring agen...

AI Technical Summary

Problems solved by technology

[0006] At present, large-scale lactic acid fermentation adopts batch fermentation, which has problems such as low equipment utilization rate, long fermentation cycle, and low production rate; some semi-continuous fermentation and a few continuous fermentation modes lead to low optical purity with the increase of continuous fermentation batches, which affects polymerization. Lactic acid production

At the same time, microbial growth requires a certain adjustment period, and an excessively long adjustment period is an important factor leading to long fermentation cycles and low production rates

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