Culture medium for culture expanding and concentration of oocystis species

A culture medium and oocyst technology, applied in the direction of microorganism-based methods, single-cell algae, and adding compounds to stimulate growth, etc., can solve the problems of high algae mortality, insufficient growth speed, and insufficient production vigor of oocysts, etc. problem, to achieve the effect of improving growth activity, improving stimulation and promoting effect, and reducing the mortality of algae species

Active Publication Date: 2021-03-30
武汉市中易天地物联科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the culture medium for oocyst algae species only contains the nutrients required by conventional oocysts, and the production vigor of oocysts is not enough, and the growth rate is not fast enough, and the mortality rate of algae growth is still high

Method used

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  • Culture medium for culture expanding and concentration of oocystis species
  • Culture medium for culture expanding and concentration of oocystis species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 50g / L, CH 4 ON 2 20g / L, sodium dihydrogen phosphate dihydrate 2g / L, ferric chloride hexahydrate 2g / L, cyclodextrin glucosyltransferase 2g / L, the B nutrient solution includes the following components and concentrations: citric acid 3g / L L. Ferric ammonium citrate 3g / L, calcium chloride dihydrate 20g / L, tetrahydrofurfuryl alcohol 3g / L, α-ionol 1g / L, boric acid 2g / L, MnCl 2 4H 2 O0.5g / L, ZnSO 4 ·7H 2 O0.2g / L, Na 2 MoO 4 2H 2 O0.3g / L, CuSO 4 ·5H 2 O 0.5g / L, Co(NO 3 )2·6H 2 O0.3g / L, vitamin B1 0.1g / L, vitamin B12 0.005g / L, biotin 0.003g / L.

[0024] As a further configuration of the present invention, the weight ratio of the A nutrient solution to the B nutrient solution in the culture medium for expanding an...

Embodiment 2

[0027] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 180g / L, CH 4 ON 2 20-50g / L, sodium dihydrogen phosphate dihydrate 6g / L, ferric chloride hexahydrate 3g / L, cyclodextrin glucosyltransferase 6g / L, the B nutrient solution includes the following components and concentrations: citric acid 8g / L, ferric ammonium citrate 8g / L, calcium chloride dihydrate 50g / L, tetrahydrofurfuryl alcohol 5g / L, α-ionol 5g / L, boric acid 3g / L, MnCl 2 4H 2 O 3g / L, ZnSO 4 ·7H 2 O 0.6g / L, Na 2 MoO 4 2H 2 O 0.8g / L, CuSO 4 ·5H 2 O 0.8g / L, Co(NO 3 )2·6H 2 O 0.6g / L, vitamin B1 0.3g / L, vitamin B12 0.001g / L, biotin 0.005g / L.

[0028] As a further configuration of the present invention, the weight ratio of the nutrient solution A and the nutrient solution B in the culture medium for expandi...

Embodiment 3

[0031] The invention provides a kind of culture medium that is used for expanding and concentrating oocyst algae species, comprising A nutrient solution and B nutrient solution, and the A nutrient solution includes the following components and concentrations: NaNO 3 100g / L, CH 4 ON 2 35g / L, sodium dihydrogen phosphate dihydrate 4g / L, ferric chloride hexahydrate 2.5g / L, cyclodextrin glucosyltransferase 4g / L, the B nutrient solution includes the following components and concentrations: citric acid 5g / L, ferric ammonium citrate 5g / L, calcium chloride dihydrate 35g / L, tetrahydrofurfuryl alcohol 4g / L, α-ionol 3g / L, boric acid 2.5g / L, MnCl 2 4H 2 O 2g / L, ZnSO 4 ·7H 2 O 0.4g / L, Na 2 MoO 4 2H 2 O 0.5g / L, CuSO 4 ·5H 2 O 0.6g / L, Co(NO 3 )2·6H 2 O 0.4g / L, vitamin B1 0.2g / L, vitamin B12 0.003g / L, biotin 0.004g / L.

[0032]As a further configuration of the present invention, the weight ratio of the A nutrient solution to the B nutrient solution in the culture medium for expandi...

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Abstract

The invention provides a culture medium for culture expanding and concentration of oocystis species, wherein the culture medium comprises a nutrient solution A and a nutrient solution B. The nutrientsolution A comprises the following components: NaNO3, sodium dihydrogen phosphate dihydrate, disodium EDTA and ferric chloride hexahydrate. The nutrient solution B comprises the following components:citric acid, ferric ammonium citrate, calcium chloride dihydrate, boric acid, manganese chloride, zinc sulfate, sodium molybdate, copper sulfate, cobalt nitrate, vitamin B1, vitamin B12 and biotin. The invention provides a culture medium for culture expanding and concentration of oocystis species rapid in growth of oocystis species, strong in growth vigor and low in death rate.

Description

technical field [0001] The invention relates to the technical field of aquaculture microorganism cultivation, in particular to a culture medium for expanding and concentrating oocyst algae species. Background technique [0002] Oocysts are common planktonic green algae that exist in various waters in China, belonging to Chlorophyta, Chlorophyceae, Chlorococcuses, Oocystaceae, and can be distributed in fresh water. The plant body is a spherical or elliptical spherical group, and the group is usually composed of 2, 4 or 8 cells, and there is a gelatinous coating composed of pectin outside the group. The algae cells are oval or oval, with a length of 10 μm-19 μm and a width of 9.0 μm-13 μm. Generally, 2, 4, 8 or 16 spore-like spores can be produced. [0003] In the prior art, the medium for oocyst algae species only contains the nutrients required by conventional oocysts, and the production activity of oocysts is not enough, and the growth rate is not fast enough, and the mort...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38Y02A40/80
Inventor 唐宾国谢敏郭为妮王继岩
Owner 武汉市中易天地物联科技有限公司
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