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Novel retinal vein obstruction mouse model construction method

A retinal vein, mouse model technology, applied in the field of biomedicine, can solve the problems of lack of mouse retina, edema, rabbit retinal leakage, etc., to reduce the interference of eye tissue, optimize the experimental operation process, and keep the refractive medium clear Effect

Active Publication Date: 2021-03-30
上海谋始生物科技有限公司
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Problems solved by technology

Although a research group has reported that DL-AAA intravitreal injection caused retinal leakage in rabbits, there is a significant difference between the leakage and the clinical manifestations of RVO, and there is still no evidence that DL-AAA can cause retinal edema in mice. system Research

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  • Novel retinal vein obstruction mouse model construction method
  • Novel retinal vein obstruction mouse model construction method
  • Novel retinal vein obstruction mouse model construction method

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Embodiment Construction

[0026] In order to clearly illustrate the technical characteristics of the present solution, the present invention will be described in detail below through specific implementation modes and in conjunction with the accompanying drawings.

[0027] 1. Construction of a new mouse animal model of RVO:

[0028] Methods and materials: Construction of a new RVO model: Prepare 8 mg / mL Bengal red solution, select 8-week-old C57 / B6 mice, and inject 66 mg / kg Bengal red into the tail vein of each mouse after anesthesia, within 30s after injection Argon green laser is used to irradiate the most exact two retinal veins at a distance of 500um from the optic disc (about 2 optic disc sizes), the specific parameters are as follows: wavelength: 532nm, laser spot diameter 50um, laser energy 50mW, duration 3s*2 times, with an interval of 0.1s, for a total of 6s. Intravitreal injection of DL-AAA was performed immediately after photodynamic intervention. The retinal thickness of the mouse models i...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a novel retinal vein obstruction mouse model construction method. The novel retinal vein obstruction mouse model construction method comprises the step of intervening a mouse by adopting a photodynamic method and then conducting injection of a Muller cell metabolism inhibitor into a vitreous body. A retinal vein obstruction mouse model prepared by the construction method is stable, can realize lasting retinal edema, can be used for conveniently verifying a drug target and an action mechanism of candidate drugs afterinjection, and provides a new generation of animal model tool for research of retinal vein obstruction diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for constructing a novel retinal vein occlusion mouse model. Background technique [0002] Retinal vein occlusion (RVO) is one of the most common retinal vascular diseases in ophthalmology. Its incidence rate is second only to diabetic retinopathy, and approximately 14.3 million adults are affected worldwide. RVO can be divided into branch retinal vein occlusion (BRVO), central retinal vein occlusion (CRVO) and hemi-central retinal vein occlusion (HCRVO) according to the location of the obstruction. RVO is usually caused by thrombosis. As the disease progresses, it may lead to complications such as macular edema (ME) and neovascular glaucoma secondary to RVO, resulting in severe vision loss and even blindness. Currently, the treatment methods for ME secondary to RVO include macular grid laser photocoagulation, intraocular injection of long-acting steroid drugs, and ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2267/0375A01K2227/105A01K2207/20A01K2207/35
Inventor 朱玮吴岩
Owner 上海谋始生物科技有限公司
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