Preparation method of peanut peptide

Abstract

The invention belongs to the technical field of peanut protein processing, and particularly relates to a preparation method of peanut peptide. The preparation method of the peanut peptide comprises the following steps of: S1) preparing peanut protein isolate; S2) performing ultrasonic enzymolysis treatment to obtain enzymatic hydrolysate; S3) performing electrodialysis desalination: adding the enzymatic hydrolysate obtained in the step S2 into a fresh water chamber of an electrodialysis device, adding 8-12g / L of a sodium sulfate aqueous solution into an electrode water chamber, adding a compound solution into a concentrated water chamber, applying 20-30V direct current voltage to electrodes on two sides of the electrodialysis device, and stopping when the conductivity is reduced to 100 microseconds / cm; and S4) performing drying: collecting feed liquid in the fresh water chamber in the step S3, and performing drying to obtain the peanut peptide. According to the preparation method disclosed by the invention, peanut meal is sequentially subjected to the steps of alkali extraction, acid extraction, ultrasonic assisted enzymolysis and electrodialysis desalination to obtain the peanut peptide, so that the protein retention rate of the peanut peptide is greatly increased, and the peanut peptide is good in antioxidant activity.

Description

technical field [0001] The invention belongs to the technical field of peanut protein processing, and in particular relates to a preparation method of peanut peptide. Background technique [0002] Peanut peptide is a small molecular amino acid polymer with high biological activity obtained by enzymatically hydrolyzing peanut protein with specific enzymes. It contains 8 essential amino acids for the human body, and can exhibit the physiological functions of protein to quickly supplement nutrition and enhance immunity. The role of force. [0003] The enzymatic hydrolysis methods of peanut peptides include single-enzyme method, double-enzyme method, ultrasonic or microwave-assisted enzymatic method. It is inevitable to add acid or alkali in these methods. Adjust the most suitable pH value accurately, and such an operation will lead to too high salt content in the enzymatic hydrolysis solution, which will eventually affect the taste and functional activity of peanut peptides. ...

AI Technical Summary

Problems solved by technology

Although protein (peptide) powder can be prepared according to this method, existing studies also show that: in electrodialysis desalination, as time goes on, the salt concentration in the concentrated water chamber increases gradually, and when the salt in the concentrated water chamber The concentration is higher than that in the fresh water chamber, which will cause concentration diffusion and loss of protein peptides

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