Crop rhizosphere soil conditioner and preparation method and application thereof
A rhizosphere soil and conditioner technology, applied in the agricultural field, can solve problems such as poor root growth, and achieve the effects of solving the deterioration of soil environment, improving crop resistance, and promoting robust growth.
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[0025] The present invention also provides a preparation method of the crop rhizosphere soil conditioner described in the above technical scheme, comprising the following steps:
[0026] 1) Paenibacillus polymyxa, Bacillus licheniformis and Bacillus lateralis are inoculated in the expansion medium and cultured, when the total microbial content in the culture medium is 2×10 9 When the cfu / mL is above, aerobic fermentation is carried out after the culture solution is mixed with the EM bacterial solution to obtain a composite bacterial solution, and the total microbial content in the composite bacterial solution is 2.2×10 9 More than cfu / mL;
[0027] 2) mixing the composite bacterial liquid obtained in the step 1), soybean powder, brown sugar and fecal water, fermenting at 10-35°C for 8-20 days to obtain a fermented liquid, filtering the fermented liquid to obtain a filtrate;
[0028] 3) mixing the filtrate obtained in the step 2) with the compound endomycorrhizal fungus, and st...
Embodiment 1
[0051] Paenibacillus polymyxa is cultured conventionally to obtain Paenibacillus polymyxa bacterial liquid; Bacillus licheniformis is cultured conventionally to obtain bacterial liquid of Bacillus licheniformis;
[0052] Insert the prepared Paenibacillus polymyxa liquid, Bacillus licheniformis liquid and Bacillus lateralis into the fermentation tank, and the inoculation ratio is 5%. 0.3g / L, corn flour 0.5g / L, ammonium sulfate 0.05g / L, sodium acetate 0.05g / L, magnesium sulfate 0.02g / L and potassium dihydrogen phosphate 0.02g / L, the temperature is controlled at 30°C, every Stir once every 3 hours, 15 minutes each time, and sample the number of microorganisms at the same time, when the number of mixed bacteria reaches 10 6 cfu ml -1 Add EM active bacterial liquid (1:1; V:V) at the time, cultivate under aerobic fermentation conditions, control the temperature at 30°C, stir once every 3 hours, 15 minutes each time, and sample the number of microorganisms at the same time, when the...
Embodiment 2
[0060] On June 6, 2019, pakchoi seedlings were planted in the water-saving park of Hohai University on June 18, 400m 2 used in experiments where the control 100m 2 , the treatment group occupies 300m 2 . When planting, every acre of crop rhizosphere soil conditioning agent 10kg (prepared in embodiment 1) diluted 2000 times spraying with preparation, after field planting 25 days, per acre of crop rhizosphere soil conditioner 8kg (prepared in embodiment 1) was diluted 1500 times and sprayed again Shower once, set three times in parallel. Soil conditioners were applied proportionally according to the planting area. Control: Spray the same amount of water, 45 days after planting. The growth potential was measured, and the results are shown in Table 1.
[0061] Table 1 Growth potential measurement results
[0062] Leaf dry weight g / plant Total dry weight g / plant Leaf biomass g / plant control 2.08 2.42 22.89 processing 1 2.98 3.01 32.1 proces...
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