Culture medium for primary isolation of porphyromonas gingivalis as well as preparation method and application of culture medium

A technology of Porphyromonas gingivalis and Porphyromonas gingivalis, which is applied in the field of culture medium for primary isolation of Porphyromonas gingivalis and its preparation, can solve the problems of difficulty and long time for Porphyromonas gingivalis, and shorten the cultivation period , promote growth, the effect of a simple formula

Active Publication Date: 2021-03-16
THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide a culture medium for primary isolation of Porphyromonas gingivalis, to solve the problems of difficulty and long time in the prior art for primary isolation of Porphyromonas gingivalis

Method used

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  • Culture medium for primary isolation of porphyromonas gingivalis as well as preparation method and application of culture medium
  • Culture medium for primary isolation of porphyromonas gingivalis as well as preparation method and application of culture medium
  • Culture medium for primary isolation of porphyromonas gingivalis as well as preparation method and application of culture medium

Examples

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preparation example Construction

[0042] The embodiment of the present invention also provides a preparation method of a culture medium for primary isolation of Porphyromonas gingivalis, comprising the following steps:

[0043] Step S1: Mix the mixed peptone, yeast extract powder, sodium chloride, agar, glucose, sodium bicarbonate, L-cysteine ​​salt, and soluble sodium pyrophosphate in proportion, add water to 1L, and mix well to obtain a mixture solution;

[0044] Step S2: Autoclave the mixture solution, and then cool down;

[0045] Step S3: Add heme storage solution, vitamin storage solution and sterile defibrinated sheep blood to the cooled mixture solution in proportion, and mix well to obtain a liquid medium;

[0046] Step S4: Pour the liquid culture medium onto a plate and cool it down to obtain a solid culture medium.

[0047] Preferably, in step S2, the mixture solution is autoclaved at a temperature of 120-125°C for 10-20 minutes; the cooling treatment step of the mixture solution includes: after th...

Embodiment 1

[0062] In this example, the culture medium for the primary isolation of Porphyromonas gingivalis includes, by weight components, 15 parts of mixed peptone, 6 parts of yeast extract powder, 2.5 parts of sodium chloride, 15 parts of agar, 1.0 part of glucose, 0.4 part of sodium bicarbonate, 0.5 part of L-cysteine ​​salt, 0.25 g of soluble sodium pyrophosphate, 0.0005 part of heme, 0.00005 part of vitamin K, 1000 parts of water and 10 parts of sterile defibrinated sheep blood.

[0063] Wherein, the preparation method of the medium for primary isolation of Porphyromonas gingivalis is as follows:

[0064] Step S1: Mix the mixed peptone, yeast extract powder, sodium chloride, agar, glucose, sodium bicarbonate, L-cysteine ​​salt, and soluble sodium pyrophosphate according to the above ratio, add water to make up to 1L, and mix well to obtain mixture solution;

[0065] Step S2: Place the mixture solution in a sterilizer for 121°C sterilization for 15 minutes. When the sterilizer indi...

Embodiment 2

[0075] The difference between this example and Example 1 is that the culture medium for primary isolation of Porphyromonas gingivalis contains different amounts of components.

[0076] Specifically, in this example, the culture medium for the primary isolation of Porphyromonas gingivalis is based on components by weight, mixed with 10 parts of peptone, 5 parts of yeast extract powder, 2.0 parts of sodium chloride, 10 parts of agar, and 0.5 parts of glucose. 0.3 parts of sodium bicarbonate, 0.4 parts of L-cysteine ​​salt, 0.15 g of soluble sodium pyrophosphate, 0.004 parts of heme, 0.0004 parts of vitamin K, 700 parts of water and 7 parts of sterile defibrinated sheep blood. The preparation method of the culture medium for the primary isolation of Porphyromonas gingivalis and the method for the primary isolation and screening of Porphyromonas gingivalis are the same as in Example 1, and will not be repeated here. in, Figure 5 It is the result picture of the uninoculated sampl...

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Abstract

The invention relates to the technical field of biology, and discloses a culture medium for primary isolation of porphyromonas gingivalis as well as a preparation method and application of the culturemedium. The culture medium for primary isolation of the porphyromonas gingivalis comprises the following components in parts by weight: 10-20 parts of mixed peptone, 5-10 parts of yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 part of glucose, 0.2-0.8 part of sodium bicarbonate, 0.1-0.5 part of L-cysteine salt, 0.1-0.5 part of soluble sodium pyrophosphate, 0.0001-0.0005 part of heme, 0.00001-0.00005 part of vitamin K, 500-1000 parts of water and 5-10 parts of sterile defibrinated sheep blood. With adoption of the culture medium for primary isolation of the porphyromonas gingivalis provided by the embodiment of the invention, the culture period of primary isolation of the porphyromonas gingivalis is greatly shortened, and rapid primary isolation of the porphyromonas gingivalis can be realized.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a culture medium for primary isolation of Porphyromonas gingivalis, a preparation method and application thereof. Background technique [0002] Periodontitis is a common chronic inflammatory disease of the oral cavity, which mostly occurs in people over 35 years old. Caused by microbial infection of the gums and periodontal supporting tissues. Among them, the "red complex" is an important factor leading to periodontitis. Porphyromonas gingivalis (Pg) is a kind of Gram-negative anaerobic bacteria, and Pg is distributed in human oral cavity, digestive system, cardiovascular system, brain and other parts. According to research reports, Pg, as one of the "cornerstone" bacteria of the "red complex", can interact with host cells in various ways to cause inflammation at the lesion site, and is associated with oral inflammation, oral and maxillofacial tumors, digestive tract tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02C12R2001/01C12Q1/045
Inventor 高社干谷变利兰子君刘轲
Owner THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
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