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Method for increasing polyphenol content in tartary buckwheat by high-pressure coupling germination

A technology of phenolic content and tartary buckwheat is applied in the field of plant stress intervention, which can solve the problem that the polyphenol content cannot be effectively increased, and achieve the effects of improving health care function, reducing the loss of seed nutrition, and improving the activity of PAL.

Active Publication Date: 2021-03-16
XIHUA UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problem that the polyphenol content in tartary buckwheat cannot be effectively increased in the prior art, the present invention provides a method for high-pressure coupled germination to increase the polyphenol content in tartary buckwheat. Seed nutrient loss increases the content of polyphenols in tartary buckwheat

Method used

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  • Method for increasing polyphenol content in tartary buckwheat by high-pressure coupling germination
  • Method for increasing polyphenol content in tartary buckwheat by high-pressure coupling germination

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Embodiment 1

[0018] Embodiment 1: Weigh four portions of 10 g of tartary buckwheat, mark them as 0MP, 20mp, 25MP, and 30MP respectively, put them into a vacuum bag and evacuate and seal them. Put the tartary buckwheat flakes marked with 20mp, 25mp, and 30mp into the ultra-high pressure equipment for 5 minutes (of course, the processing time can also be 1 minute, 2 minutes, or 3 minutes. are the same, so in order to save space and reduce unnecessary repetition, only 5 minutes are selected as an example). Soak the treated tartary buckwheat with 1% sodium hypochlorite for 10 minutes, wash until neutral, add 50°C hot water for heat treatment, and cool to room temperature. Soaked tartary buckwheat was germinated at a constant temperature of 25°C for 48h, watered every 12h, and the germinated buckwheat was dried at 50°C to analyze the composition changes. Such as figure 1 As shown, after 48 hours of germination, all four groups of tartary buckwheat germinated. Compared with the control group ...

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Abstract

The invention discloses a method for increasing polyphenol content in tartary buckwheat by high-pressure coupling germination. The method comprises the following steps of (1) putting tartary buckwheatseeds into a vacuum bag, and conducting vacuumizing and sealing; (2) putting the tartary buckwheat seeds into ultrahigh-pressure equipment for high-pressure treatment; (3) soaking the tartary buckwheat seeds in a sodium hypochlorite solution with the mass concentration of 1%; (4) washing to be neutral, and then adding hot water for heat treatment; and (5) cooling the tartary buckwheat seeds to room temperature after heat treatment, then sprouting under a constant temperature condition, and watering and supplementing water in the sprouting process. The method has the advantages that the content of vanillic acid, hypericin, aromadendrin, catechin, acetosyringone and catechuic acid can be remarkably increased under the stress action of 20 MP-30 MP ultrahigh pressure, and the health-care function of the tartary buckwheat in the aspect of oxidation resistance is improved.

Description

technical field [0001] The invention belongs to the technical field of plant stress intervention, and specifically relates to a method for increasing the polyphenol content of tartary buckwheat during germination through high pressure stress. Background technique [0002] Tartary buckwheat belongs to the Polygonaceae family and originated in western China and the Himalayas. Buckwheat is rich in nutrition and is a kind of food crop with both medicine and food. Buckwheat flavonoids are important bioactive components, which have strong antioxidant and free radical scavenging capabilities. According to reports, buckwheat is rich in flavonoids, the main components of which are rutin, catechin, and quercetin. In recent years, there have been more and more studies on seed germination. With the rapid content changes in the germination process, the components and contents of various substances in seeds also change accordingly, especially the metabolites such as lipids, amino acids, ...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 李伟丽吴韬肖丽张萍
Owner XIHUA UNIV
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