A biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis

A biomarker, gastritis patient technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problem of not finding markers for detecting gastric cancer autoantibodies, etc.

Active Publication Date: 2022-07-26
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cancer autoantibodies are the specific responses of the immune system against cancer cells, and have the characteristics of high sensitivity and high specificity. However, no markers have been found that can detect gastric cancer autoantibodies from patients with gastritis.

Method used

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  • A biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis
  • A biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis
  • A biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The embodiments of the present application provide a screening test for detecting a combination of biomarkers for gastric cancer autoantibodies from gastritis patients, and the specific steps are as follows:

[0043] 1. Construction of a protein chip for the detection of gastric cancer autoantibodies from gastritis patients

[0044] 1. In this example, genetic engineering methods are used to produce and identify a variety of human proteins using prokaryotic and eukaryotic expression platforms. The target gene is amplified by RT-PCR and cloned into a bacterial expression vector. After the correctness of the gene cloning vector is verified by DNA sequencing, the recombinant material is transformed into competent expression bacteria. After gene induction and expression, a variety of proteins were obtained by affinity chromatography and high performance liquid chromatography purification from the lysate or inclusion bodies.

[0045] 3. A variety of proteins are immobilized...

Embodiment 2

[0050] The examples of this application provide a confirmatory test for detecting gastric cancer autoantibodies biomarker combination ADAM17, MIP-1β and MMP-7 from gastritis patients. The specific steps are as follows:

[0051] 1. The test samples were selected from patients with chronic gastritis (77 cases in total) and patients with diffuse gastric cancer (47 cases in total), the serum of the above samples was extracted, and the serum IgA and IgG of the above 124 samples were obtained.

[0052] 2. The IgA and IgG of the serum of the 124 samples in step 1 were respectively immunoreacted with the protein chip for gastric cancer diagnosis in Example 1, and then the anti-IgG antibody and anti-IgA conjugated with detectable biotin were respectively The corresponding antibodies were incubated with the above chips, and the responses of the serum IgA and IgG of 124 samples to the biomarkers (hereinafter referred to as targets) of the protein chip for gastric cancer diagnosis were obt...

Embodiment 3

[0058] The examples of this application provide a confirmatory test for detecting gastric cancer autoantibodies biomarker combination ADAM17, MIP-1β and MMP-7 from gastritis patients. The specific steps are as follows:

[0059] 1. The test samples were selected from patients with chronic gastritis (77 cases in total) and patients with intestinal type gastric cancer (77 cases in total).

[0060] 2. The IgA and IgG of the serum of the 154 samples in step 1 were immunoreacted with the protein chip for gastric cancer diagnosis in Example 1, respectively, and then the anti-IgG antibody and anti-IgA conjugated with detectable biotin were respectively The corresponding antibodies were incubated with the above chips, and the responses of serum IgA and IgG of 154 samples to the biomarkers (hereinafter referred to as targets) of the protein chip for gastric cancer diagnosis were obtained, and the IgA response signal values ​​corresponding to the respective biomarkers were obtained. and ...

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Abstract

The present application belongs to the technical field of molecular biology, and particularly relates to a biomarker combination and application for detecting gastric cancer autoantibodies in gastritis patients. The present application can detect autoantibodies in gastric cancer patient serum in gastritis patients. The present application provides a biomarker combination for detecting gastric cancer autoantibodies in patients with gastritis, including disintegrin-metalloproteinase-17, macrophage inflammatory protein-1β and matrix metalloproteinase-7. The present application provides a biomarker combination for detecting gastric cancer autoantibodies in gastritis patients, which can effectively solve the technical defects of low specificity, sensitivity and accuracy of existing markers for detecting gastric cancer autoantibodies.

Description

technical field [0001] The present application belongs to the technical field of molecular biology, and particularly relates to a biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world and one of the malignant tumors with the highest mortality rate. Most patients with gastric cancer have missed the best time for diagnosis and treatment when they are diagnosed, leading to disease progression, tumor metastasis, and even end-stage disease. From the perspective of TMN staging of gastric cancer, the 5-year survival rate of gastric cancer is 97.6% in the early stage of the first stage, 94.9% in the late stage of the first stage, 70.49% in the second stage, 56.7% in the early stage of the third stage, and 56.7% in the late stage of the third stage. was 31.9%, and the fourth period was 6.5%. It can be seen that the diagnosis of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/573
CPCG01N33/57488G01N33/57446G01N33/6854G01N33/573
Inventor 黄若磐罗树红符聪聪琼·克拉布特里
Owner RAYBIOTECH INC GUANGZHOU
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