LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) determination method of aloesin in rat plasma

A kind of aloe verain and determination method technology, applied in the field of aloe verain, can solve the problems of backward analysis technology, less aloe verain, no pharmacokinetic parameters provided, etc., and achieve the effect of accurate detection

Active Publication Date: 2021-02-26
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the pharmacokinetics of aloesin, the relevant reports do not provide pharmacokinetic parameters, and the analytical techniques used are relatively backward.

Method used

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  • LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) determination method of aloesin in rat plasma
  • LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) determination method of aloesin in rat plasma
  • LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) determination method of aloesin in rat plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1 Instruments, reagents and experimental animals

[0033] 1.1 Instrument

[0034] Nexera XR UHPLC liquid chromatograph (Shimadzu Corporation, Japan) is connected in series with AB-SCIEX API 4000+ quadrupole mass spectrometer through Turbo V ion source interface, and Analyst software controls data acquisition and processing (AB Corporation, USA). 5922 type refrigerated centrifuge (KUBOTA, Japan), XS105DU 1 / 100,000 electronic analytical balance (Mettler-Toledo, Switzerland), LabTower EDI15 ultra-pure water machine (Thermo, USA), Vibrax circular oscillator (Aika, Germany ). AMS small animal anesthesia machine (Beijing Gene & I).

[0035] 1.2 Drugs and experimental animals

[0036] The aloesin reference substance was provided by Hao Rui Chemical (Shanghai) Co., Ltd.; the aloeresin D (Aloeresin D) reference substance was provided by Chengdu Pufeide Biotechnology Co., Ltd., batch number: 19072605. The purity of the reference substances were all ≥98%. Chromatographic grad...

Embodiment 2

[0082] The difference between this example and Example 1 is that mobile phase A is 1.0‰ (v / v) formic acid aqueous solution. After testing, the results showed that the peak shape of aloin and aloin D was good without mutual interference, and the endogenous substances in plasma had no obvious influence on the signals of aloin and aloin D.

Embodiment 3

[0084] The difference between this example and Example 1 is that the concentration of the methanol solution containing the internal standard aloin D is 100 ng / mL. After testing, the results showed that the peak height and peak area of ​​aloin D increased by about 1 times, which can be used for the effective quantification of aloesin.

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Abstract

The invention provides an LCMS / MS determination method of aloesin in rat blood plasma, which comprises the following steps: (1) blood plasma sample treatment: taking blood plasma to be detected containing aloesin, adding a methanol solution containing internal standard aloin D, carrying out vortex precipitation, centrifuging the mixture, and collecting the supernatant as a sample to be detected; (2) setting liquid chromatography conditions as follows: a mobile phase A and a mobile phase B are adopted for gradient elution, the mobile phase A is an aqueous solution containing 0.01-1.0% o (v / v) of formic acid, and the mobile phase B is methanol; and (3) setting mass spectrum conditions as follows: an electrospray ion source, negative ion detection, spray voltage of 4500V, spray temperature of550 DEG C, a multi-reaction ion monitoring scanning mode, an ion pair for quantitative analysis of aloesin of m / z 393.1 -> 272.9, and an internal standard compound aloin D ion pair of m / z 555.3 -> 144.9. The method provided by the invention can be used for detecting aloesin in blood plasma more quickly, sensitively and accurately.

Description

technical field [0001] The invention relates to the technical field of aloesin, in particular to an LC-MS / MS determination method for aloesin in rat plasma. Background technique [0002] Aloepicrin is a kind of chromone glycosides, which exists in various plants of the genus Aloe in Liliaceae, and the content in Aloe barbadensis Miller is about 3‰. Aloe vera is a traditional Chinese medicine with a long history of application. The juice of its leaves is concentrated and dried before being used as medicine. Relevant studies have shown that aloesin is one of the important active substances of aloe. Aloesin can inhibit the activity of tyrosinase, prevent the synthesis of melanin, and has whitening effect. Aloesin can regulate the release of inflammatory factors and growth factors in cells, produce anti-inflammatory effects; induce the activation of Smad and MAPK signaling proteins, and promote wound healing. Aloepicrin can also activate the WNT signaling pathway, down-regula...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N30/34G01N30/7233
Inventor 谭银丰李海龙李友宾王雪松崔雪
Owner HAINAN MEDICAL COLLEGE
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