Preparation method and application of sipunculus nudus polysaccharide
A naked square starworm, square starworm technology, applied in the field of medicine, can solve the problems of time-consuming, inconvenient, and inability to effectively absorb nutrients, and achieve the effect of being easy to carry, easy to carry, and improve bioavailability
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Embodiment 1
[0040] This embodiment provides a method for preparing the polysaccharide of the naked square star insect, the preparation method comprising the following steps:
[0041] (1) Take fresh naked star worms, the body weight of the selected star worms is 15g, remove silt and viscera and clean them, and then homogenate to obtain a homogenate;
[0042] (2) Add deionized water at 105°C to the homogenate obtained in step (1), the volume ratio of the homogenate to deionized water is 1:3, extract for 5.5 hours, filter to remove insoluble components, and then vacuum concentrate the filtrate to the original 1 / 4 of the volume, and then add absolute ethanol accounting for 4 times the volume of the filtrate to the filtrate, let it stand overnight, then centrifuge at a temperature of 6°C and a speed of 7500rpm for 6min, pour out the supernatant and collect the precipitate ;
[0043] (3) Wash the precipitate obtained in step (2) with absolute ethanol for 3 times, then centrifuge, collect the was...
Embodiment 2
[0046] This embodiment provides a method for preparing the polysaccharide of the naked square star insect, the preparation method comprising the following steps:
[0047] (1) Take fresh naked star worm, the weight of the selected star worm is 12g, remove the sediment and viscera and wash, then homogenate to obtain homogenate;
[0048] (2) Add deionized water at 100°C to the homogenate obtained in step (1), the volume ratio of the homogenate to deionized water is 1:2, extract for 5.0 hours, filter to remove insoluble components, and then concentrate the filtrate in vacuum to the original 1 / 4 of the volume, then add absolute ethanol accounting for 4 times the volume of the filtrate to the filtrate, let it stand overnight, then centrifuge at a temperature of 5°C and a speed of 7000rpm for 5min, pour out the supernatant and collect the precipitate ;
[0049] (3) Wash the precipitate obtained in step (2) with absolute ethanol for 2-3 times, centrifuge again, collect the washed pre...
Embodiment 3
[0052] This embodiment provides a method for preparing the polysaccharide of the naked square star insect, the preparation method comprising the following steps:
[0053] (1) Take fresh naked star worms, the body weight of the selected star worms is 10-15g, remove the sediment and viscera and clean them, and then homogenate to obtain a homogenate;
[0054] (2) Add deionized water at 95°C to the homogenate obtained in step (1), the volume ratio of the homogenate to deionized water is 1:2, extract for 4.5 hours, filter to remove insoluble components, and then vacuum concentrate the filtrate to the original 1 / 4 of the volume, then add absolute ethanol accounting for 4 times the volume of the filtrate to the filtrate, let it stand overnight, then centrifuge for 4min at a temperature of 4-6°C and a speed of 6500rpm, pour out the supernatant and collect sediment;
[0055] (3) Wash the precipitate obtained in step (2) twice with absolute ethanol, centrifuge again, collect the washed...
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