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Affinity agent of TIGIT-IgV and application thereof

A technology for affinity agents and diagnostic kits, applied in the field of affinity agents for TIGIT-IgV, can solve the problems of being easily degraded by enzymes and difficult to develop

Active Publication Date: 2021-02-26
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the existing peptide drugs targeting immune checkpoint molecules are in the natural L configuration, which is easily degraded by enzymes in the body and difficult to exert its due effect

Method used

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  • Affinity agent of TIGIT-IgV and application thereof
  • Affinity agent of TIGIT-IgV and application thereof
  • Affinity agent of TIGIT-IgV and application thereof

Examples

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Embodiment Construction

[0051] Embodiments of the present invention will be described in detail below in conjunction with examples, but the following examples are only used to illustrate the present invention, and should not limit the scope of the present invention.

[0052] Unless otherwise specified, the reagents, biological materials, culture media and solutions used below are commonly used in the art, publicly available or commercially available.

[0053] 1. The parent peptides such as the affinity peptide TBP-1 of TIGIT-IgV were obtained by liquid phase screening of the phage mirror display peptide library. The general screening process is as follows:

[0054] a) D-TIGIT-IgV-biotin was fully chemically synthesized, and the D-configuration protein was captured by streptavidin (SA) magnetic beads, and the screening of the phage-displayed dodecapeptide library was carried out by differential liquid phase screening;

[0055] b) After multiple rounds of screening, phage monoclonals with affinity to t...

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Abstract

The invention relates to an affinity agent of TIGIT-IgV and application thereof. The affinity agent of the TIGIT-IgV is selected from amino acids with sequence shown as SEQ ID NO. 1, 3 or 4, or structure shown as formula I or II, namely X1-Tyr-X2-His-X3-Arg-X4-X5 (I) or X1-Tyr-X2-His-X3-Arg-X4-X5-(L)n-X6 (II). The affinity agent of the TIGIT-IgV is found out by performing repeated screening and optimizing in a unique way, and is capable of relatively well blocking interaction between TIGIT and PVR, thereby treating tumors or other types of diseases.

Description

Technical field: [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to an affinity agent for TIGIT-IgV and its application in tumors and other related diseases. Background technique: [0002] Tumor immunotherapy, especially tumor immunotherapy based on immune checkpoint molecular blockade is the most promising research direction in the field of tumor treatment. Immune checkpoint molecules refer to a series of non-redundant negative co-stimulatory molecules expressed by T cells or NK cells, such as PD-1, TIM-3, LAG-3 and TIGIT. When the immune system is activated by external stimuli, the expression of immune checkpoint molecules is up-regulated, and binds to ligand molecules on antigen-presenting cells to play a "brake" function to prevent excessive activation of T cells. The overexpression or function of immune checkpoint molecules will mediate immune tolerance and lead to the occurrence of tumors and other diseases; on the ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C07K14/00A61K38/08A61K38/10A61K38/16A61P35/00A61P31/00A61P37/02G01N33/68
CPCC07K7/06C07K7/08C07K14/001A61P35/00A61P31/00A61P37/02G01N33/6845A61K38/00G01N2333/47
Inventor 高艳锋周秀曼李琬琼翟文杰吴亚红赵文珊祁元明
Owner ZHENGZHOU UNIV
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