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Bacillus calmette-guerin vaccine complex adsorbing nano-drug carrier by utilizing positive and negative charge characteristics and preparation method of bacillus calmette-guerin complex

A nano-drug carrier, positive and negative charge technology, used in drug combinations, pharmaceutical formulations, medical preparations with inactive ingredients, etc., can solve the problems of short drug retention time and insufficient drug absorption, and achieve good adsorption and absorption. effect, enhance uptake and tumor targeting, and improve intratumoral transport and absorption

Pending Publication Date: 2021-02-26
天津医科大学第二医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biggest problem with intravesical administration is that the drug stays in the bladder for a short time and the drug is not fully absorbed.

Method used

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  • Bacillus calmette-guerin vaccine complex adsorbing nano-drug carrier by utilizing positive and negative charge characteristics and preparation method of bacillus calmette-guerin complex
  • Bacillus calmette-guerin vaccine complex adsorbing nano-drug carrier by utilizing positive and negative charge characteristics and preparation method of bacillus calmette-guerin complex
  • Bacillus calmette-guerin vaccine complex adsorbing nano-drug carrier by utilizing positive and negative charge characteristics and preparation method of bacillus calmette-guerin complex

Examples

Experimental program
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Effect test

Embodiment

[0029] Embodiment: Taking the common clinical chemotherapeutic drug doxorubicin (Dox) as an example, it is illustrated that the BCG complex adsorbing positive Dox nano drug carrier is prepared by the following method:

[0030] 1) Preparation of positively charged PLGA-coated DOX nanoparticles:

[0031] Weigh 10 mg of organic material polylactic-co-glycolic acid (PLGA) and dissolve it in 1 ml of dichloromethane (DCM) organic phase to prepare a PLGA-DCM solution; weigh 1 mg of doxorubicin (Dox) and dissolve it in 0.1 ml of dimethyl Dox-DMSO solution was prepared in sulfoxide (DMSO). Add 0.1ml of Dox-DMSO solution to 0.9ml of PLGA-DCM solution, and in an ultrasonic water bath, oscillate ultrasonically at room temperature for 30min to make them fully mixed. Then slowly add the above-mentioned mixed solution into 1ml of 0.5% polyvinyl alcohol (PVA) solution with a mass percentage of 0.5%, and use a probe ultrasonic homogenizer to ultrasonicate for 2min (power 30W, frequency 20KHz)...

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Abstract

The invention discloses a bacillus calmette-guerin vaccine complex adsorbing a nano-drug carrier by positive and negative charge characteristics. The complex has a complex structure formed by mutual combination of the nano-drug carrier carrying positive charges and BCG thallus by means of mutual attraction of positive and negative charges, wherein the nano-drug carrier is nano-particles which arecoated by a polylactic acid-glycolic acid copolymer, are subjected to surface modification by cationic polymer polyethyleneimine and have the particle size of 80nm-100nm; and 10-20 nano particles arecombined on the surface of each BCG thallus on average. The preparation method comprises the following steps: synthesizing a cationic nano-drug carrier by using a polymeric material water / oil / water double emulsification method, and combining BCG with the cationic nano-drug carrier. According to the present invention, the bacillus calmette-guerin vaccine complex BCG has good adsorption and absorption effects on the bladder epithelium, the drug particles are loaded on the surface of the BCG thallus, and the BCG serving as the carrier can be used for enhancing uptake and tumor targeting of the nano-drug carrier in the bladder so as to significantly improve the tumor killing effect of the drug.

Description

technical field [0001] The invention belongs to the field of nano-medicine carrier and tumor immunity, in particular to the preparation of BCG complex adsorbed with nano-medicine carrier. Background technique [0002] Bladder cancer is the second most common malignant tumor in the urinary system, and non-muscle invasive (NMIBC) patients account for more than 70% of all newly diagnosed bladder cancer patients. The high rate of tumor recurrence and progression is the most important factor affecting the prognosis of bladder cancer patients. Intravesical Bacillus Calmette-Guerin ((BCG Vaccine)) infusion is the recommended postoperative treatment for high-risk NMIBC patients and has been widely used clinically. Intravesical BCG perfusion can induce a strong local immune response in the body to kill surgical residues or tiny tumor lesions, thereby significantly reducing the recurrence and progression of cancer and improving the prognosis of patients. Although the immunotherapy e...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K47/34A61K31/704A61P35/00A61P37/04
CPCA61K9/5176A61K9/5153A61K31/704A61P35/00A61P37/04
Inventor 牛远杰赵阳刘康康尚芝群孙二琳马媛王丽宁
Owner 天津医科大学第二医院
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