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Application of highland barley fermentation extract

An extract, highland barley technology, applied in the application field of highland barley fermented extract, can solve the problems of repairing aging skin without micro-ecology on the skin surface, achieve good application prospects, promote proliferation, and reduce the effect of ratio

Pending Publication Date: 2021-02-26
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no report of using the fermented barley extract as an external skin agent, nor has there been any report about the fermented barley extract being used to improve the micro-ecology of the skin surface or repair aging skin

Method used

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  • Application of highland barley fermentation extract
  • Application of highland barley fermentation extract
  • Application of highland barley fermentation extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of highland barley fermentation product filtrate

[0033] The base material of this fermentation product is highland barley, which is produced in Lhasa, Tibet, in the Pan-Himalaya region. The highland barley grains containing the bran are washed with deionized water, dried, and then beaten by a flouring machine, and passed through a 20-100 mesh sieve to obtain highland barley flour. The highland barley powder is mixed with deionized water, and the content of the highland barley powder in the fermentation medium / culture solution is 4wt%; after the high temperature and high pressure sterilization is cooled, Saccharomyces cerevisiae is fermented under the conditions of 26~30° C. and 150~250rpm 48 hours.

[0034] Preparation of highland barley fermentation filtrate:

[0035] (1) Centrifuge the fermentation product for 10-30 min at 4-10 °C and 6000 rpm, and take the supernatant;

[0036] (2) Adjust pH to 5.0~7.0, after 30~60min of water bath at 80~90...

Embodiment 2

[0041] Example 2 Influence of highland barley fermentation extract on surface flora

[0042] (1) Experimental materials

[0043] 1. Experimental strains

[0044] Staphylococcus epidermidis S.e (Staphylococcus epidermidis), Staphylococcus aureus S.a (Staphylococcus aureus).

[0045] 2. Culture medium

[0046] LB medium: 0.5% yeast powder, 1.0% tryptone, 1.0% NaCl, the balance is water, and the percentages are mass percentages. 121 ℃, 20min high temperature autoclave sterilization after cooling for use.

[0047] 3. Sample Information

[0048] Blank sample (Control): sterile physiological saline: 0.9% NaCl, the balance is water, and the percentage is mass percentage. 121 ℃, 20min high temperature autoclave sterilization after cooling for use.

[0049] Experimental sample: the highland barley fermentation product filtrate obtained in Example 1, wherein the solid content is 1.5 wt%.

[0050] (2) Experimental steps

[0051] 1. Put the preserved S.a and S.e (single colony or ...

Embodiment 3

[0056] Example 3 Cell proliferation of highland barley fermentation products

[0057] (1) Experimental materials

[0058] Cells: dermal fibroblasts Fibroblast, human immortalized epidermal cells HaCaT.

[0059] Experimental sample: The highland barley fermentation product filtrate was prepared into solutions of different concentrations with deionized water, and the highland barley fermentation product filtrate of each concentration was added to the serum-free DMEM or MEM medium required for different cell growth to dry the highland barley fermentation product filtrate in the medium. The substance content is 0.015%, 0.075% and 0.15%, and the percentages are mass percentages.

[0060] (2) Experimental method

[0061] The highland barley fermentation product filtrate was prepared into solutions of different concentrations with deionized water, and added to the culture medium of human fibroblasts. The deionized water without the sample was used as a blank control. The absorbanc...

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Abstract

The invention belongs to the field of plant extraction and the field of chemicals for daily use, and particularly relates to application of a highland barley fermentation extract. The highland barleyfermentation extract, obtained by fermenting the highland barley through saccharomyces cerevisiae and subjecting fermentation broth to centrifugation and filtration, can reduce the ratio of staphylococcus aureus / staphylococcus epidermidis and promote the proliferation of dermal fibroblasts and epidermal cells, so that the effects of regulating skin flora, maintaining and / or repairing skin surfacemicro-ecology and repairing aged skin are achieved. Therefore, the highland barley fermentation extract can be used for preparing a preparation for external use for skin, and can be used as an activeingredient for repairing aged skin and improving skin surface micro-ecology.

Description

technical field [0001] The invention belongs to the field of plant extraction and the field of daily chemicals, in particular to the application of highland barley fermented extract. Background technique [0002] Human skin fibroblasts are the most important cells in the dermal reticulum of the skin and are one of the main repair cells after skin aging and cell damage. It can not only promote the migration, proliferation and differentiation of epidermal cells, but also secrete a large amount of collagen, elastin and various cell repair factors, and has a strong self-renewal ability to repair aging skin. [0003] A large number of microorganisms are stored on the surface of human skin and in the gut, which have been with us since birth and have a symbiotic relationship with humans. Among them, the microflora on the skin surface or the surface microecology, as an important biological barrier in the outer layer of the physical barrier of the human skin, plays an important role...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61Q19/00A61Q19/08A61Q17/00C12P1/02C12R1/865
CPCA61K8/9794A61Q19/00A61Q19/08A61Q17/005C12P1/02A61K2800/85C12R2001/865C12N1/185
Inventor 李俊章漳黄俊
Owner JALA GROUP CORPORATION
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