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Application of phaaenopsis Sogo Yukidian ukid extract for preparation of composition for resisting glycation and improving appearance of skin

A Phalaenopsis, extract technology, applied in the field of plant extracts, can solve the problems of sensitivity, water retention capacity decline, etc., to achieve the effect of inhibiting non-enzyme browning, improving water retention capacity, and avoiding denaturation

Inactive Publication Date: 2021-02-23
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the above, in order to improve the skin’s fragility and sensitivity caused by damaged keratinocytes, decreased water retention capacity, and skin aging, it is necessary to develop a method that can directly and effectively improve or delay the occurrence of skin aging, while at the same time It is necessary to effectively maintain the normal physiological metabolism of keratinocytes, maintain the integrity of the stratum corneum structure, and increase the active ingredients of anti-glycation activity

Method used

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  • Application of phaaenopsis Sogo Yukidian ukid extract for preparation of composition for resisting glycation and improving appearance of skin
  • Application of phaaenopsis Sogo Yukidian ukid extract for preparation of composition for resisting glycation and improving appearance of skin
  • Application of phaaenopsis Sogo Yukidian ukid extract for preparation of composition for resisting glycation and improving appearance of skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 The preparation method of Phalaenopsis chinensis extract of the present invention

[0042] In one embodiment of the present invention, after the flower of Phalaenopsis large white is cleaned and cut into the size of each piece of 12 mm, the extraction solvent of Phalaenopsis large white after cleaning and water, alcohol, or alcohol-water mixture is taken, and the extraction solvent It is preferably water, mixed at a volume ratio of 1-5:5-20, after homogenization, extract in a solvent at 75-95°C for 0.5-3 hours, then cool to room temperature after crude extraction, and then centrifuge. Obtain the supernatant after centrifuging at 300 g for 10 minutes; then filter the supernatant with a 400-mesh filter to obtain the filtrate. Finally, the filtrate is concentrated under reduced pressure at 50-60° C. to obtain the Phalaenopsis chinensis extract of the present invention.

Embodiment 2

[0043] Example 2 Anti-glycation (AGEs) effect of Phalaenopsis chinensis extract of the present invention

[0044] This embodiment is to carry out the test of the efficacy of the anti-glycation activity of the Phalaenopsis japonica extract of the present invention, to inhibit the efficiency of D-fructose (D-fructose) to produce glycosylation of collagen (Collagen), and to carry out the quantification of saccharification activity; anti-glycation Can inhibit non-enzymatic browning to avoid denaturation of functional proteins in the body. At first, get 0.2mL concentration respectively and be that the Phalaenopsis chinensis extract solution of the present invention is 2.5%, and the water of 0.2mL as control group solution, and add the collagen protein solution of 0.2mL 60mg / mL (with the phosphate buffered saline of 200mM Solution configuration, pH7.4, which contains 0.06% sodium azide (Sodium azide, NaN 3 )) and 0.2mL of 1.5M D-fructose solution (configured with 200mM phosphate bu...

Embodiment 3

[0047] Example 3 The effect of Phalaenopsis chinensis extract of the present invention on promoting the secretion of hyaluronic acid from keratinocytes

[0048] Since it is known that keratinocytes can secrete hyaluronic acid and other substances as intercellular substances to maintain the integrity of the epidermal barrier, prevent skin moisture loss and form a complete protection, an embodiment of the present invention uses human primary epidermal keratinocytes (Human primary epidermal keratinocytes, HPEK) to carry out the test of the efficacy of the Phalaenopsis japonica extract of the present invention to promote the secretion of hyaluronic acid from keratinocytes. The human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) No. HPEK-50, and the cells were cultivated in serum-free keratinocyte culture medium (Keratinocyte-SFM) (purchased from Thermo Company, the United States, No. #17005042), and containing 5% CO 2 cultured in a 37°C cell culture incuba...

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Abstract

The invention relates to a plant extract, in particular to application of a phaaenopsis Sogo Yukidian ukid extract for preparation of a composition for resisting glycation and improving appearance ofskin. The invention provides application of a phaaenopsis Sogo Yukidian ukid extract in resisting glycation and improving appearance of skin, and application of the phaaenopsis Sogo Yukidian ukid extract in preparing a composition for improving expression quantity of a keratin 14 gene and a maternal antibody Dpp homolog gene. The phaaenopsis Sogo Yukidian ukid extract is obtained by extracting phaaenopsis Sogo Yukidian ukid with a solvent of water, alcohol or an alcohol-water mixture. The phaaenopsis Sogo Yukidian ukid extract can improve the capability of removing glycation end products, improve the expression quantity of the keratin 14 gene and SMAD1 gene, improve the hyaluronic acid secretion quantity of keratinocytes and improve the glossiness of individual skin, and is beneficial to delaying the aging progress, maintaining the arrangement of skin keratinocytes and enabling the cuticle structure of the skin to be complete; the water-retaining property of the skin is improved, and the conditions of darkness, dryness and withering of skin are improved, so that the skin is bright and glossy.

Description

technical field [0001] The invention relates to plant extracts, in particular to the use of the extract of Phalaenopsis phalaenopsis for preparing a composition for anti-glycation and improving skin appearance. Background technique [0002] The epidermis is the outermost layer of the skin. From the outside to the inside, it consists of the stratum corneum, granular layer, spinous layer, and basal layer. The epidermis is mainly formed by continuous upward differentiation of undifferentiated cylindrical keratinocytes in the basal layer. The process is called keratinization. The water content in corneocytes is high. As the cells metabolize and differentiate upward, the shape of corneocytes will gradually become flat, and the nucleus and organelles will begin to degenerate and shrink, and dead cells without nuclei and organelles will form in the stratum corneum. The main function of the epidermis is to keep the skin water and form a skin barrier to resist various external injur...

Claims

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Application Information

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IPC IPC(8): A61K36/898A61K8/9794A61Q19/00A61P17/00A61P39/00A23L33/105
CPCA61P17/00A61Q19/00A23V2002/00A23L33/105A61K36/898A61K8/9794A61P39/00A23V2200/318A23V2250/21
Inventor 林咏翔
Owner 百岳特生物技术(上海)有限公司
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