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Method for quantitatively determining abiraterone in blood and application thereof

A technology for quantitative determination of abiraterone, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost, high toxicity, and long treatment process, and achieve the use of less sample volume, no matrix interference, and low detection cost Effect

Inactive Publication Date: 2021-02-19
南京广祺医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pretreatment process of the liquid-liquid extraction method is long, the toxicity is relatively high, and the cost is high.

Method used

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  • Method for quantitatively determining abiraterone in blood and application thereof
  • Method for quantitatively determining abiraterone in blood and application thereof
  • Method for quantitatively determining abiraterone in blood and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] as attached Figure 1-9 Shown, a method for quantitative determination of abiraterone in blood, specifically comprising the following steps:

[0079] S1: Sample pretreatment

[0080] Take 20.0 μL of plasma / serum sample, add 200 μL of internal standard working solution (acetonitrile solution containing 10 ng / mL Abiraterone D4), vortex for 5 min, then centrifuge at 4°C and 4000 rpm for 10 min, take 180 μL of supernatant, and inject To the liquid mass spectrometer for measurement and analysis.

[0081] S2: Setting parameters

[0082] 1) Set the chromatographic conditions, and the elution parameters are as shown in Table 1.

[0083] Table 1

[0084] time (min) Flow rate (mL / min) Mobile phase A% Mobile phase B% 0.00 0.50 40 60 2.50 0.50 40 60

[0085] Chromatographic column: Agilent ZORBAX Eclipse Plus-C18 chromatographic column, the specification is 2.1×50mm×5μm;

[0086] Guard column: Phenomenex Security Guard Cartridges Kit C18 guar...

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Abstract

The invention discloses a method for quantitatively determining abiraterone in blood and application thereof. According to the invention, a direct protein precipitation method is adopted, sample introduction determination is carried out after centrifugation, and the concentration or content of abiraterone in blood plasma is obtained through a standard curve of abiraterone. According to the methodfor quantitatively determining abiraterone in blood plasma and the application thereof, high-throughput sample treatment is facilitated, and the method is free of toxicity and safe to operate; the method has good specificity, sensitivity, precision and accuracy; a plasma matrix does not influence an analysis result; sample consumption is low; a pretreatment process is simple, economical and rapid;analysis time is short; analysis efficiency is high; an extraction recovery rate is high; and the method can be used for new preparation development, therapeutic drug monitoring, drug interaction research and the like of abiraterone and has extremely high application value.

Description

technical field [0001] The invention relates to the technical field of chemical drug analysis, in particular to a method and application for quantitative determination of abiraterone in blood. Background technique [0002] Abiraterone (ABTL) is a CYP17 inhibitor. Clinically, abiraterone acetate (abiraterone acetyl ester) is used in combination with prednisone, mainly for the treatment of metastatic castration-resistant prostate cancer previously treated with docetaxel-containing chemotherapy. cancer patients. At present, the adverse reactions of abiraterone include: history of cardiovascular disease, hypertension, hypokalemia, fluid retention due to excessive mineralocorticoids, adrenal insufficiency, liver toxicity, etc. In addition, food has a significant impact on the absorption of abiraterone acetate; and in patients with liver damage, abiraterone should be individualized according to the exposure of abiraterone, and at the same time, abiraterone has a high plasma prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86G01N30/88
CPCG01N30/02G01N30/06G01N30/72G01N30/8675G01N30/88G01N2030/045G01N2030/067G01N2030/8822
Inventor 汤泓山莽挺祝建平张小健鞠轶廖涛梅
Owner 南京广祺医药科技有限公司
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