Immune cell storage solution, preparation method and application methods thereof

A technology of immune cells and storage solution, which is applied in the field of immune cell storage, can solve the problems of low immune cell function and low immune cell activity, and achieve the effect of convenient clinical application

Inactive Publication Date: 2021-02-19
青岛中德华大细胞科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But generally speaking, the groups receiving immune cell therapy are mainly tumor or virus-infected groups. The function of immune cells in such groups is already very low, and the activity of isolated immune cells is low.

Method used

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  • Immune cell storage solution, preparation method and application methods thereof
  • Immune cell storage solution, preparation method and application methods thereof
  • Immune cell storage solution, preparation method and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The invention provides an immune cell storage solution, each 1000ml storage solution includes the following components and contents:

[0048] Sucrose 25g;

[0049] Glycerin 50ml;

[0050] Albumin 20g;

[0051] Sodium bicarbonate 5g;

[0052] Cholesterol 0.2g;

[0053] Propylene glycol 5% mg;

[0054] Vitamin E0.6g;

[0055] Vitamin C0.6mg;

[0056] EDTA at 0.01% mg;

[0057] Phosphate buffer is 65% ml;

[0058] Sodium selenite is 0.01% mg;

[0059] The balance is cell culture medium.

Embodiment 2

[0061] The invention provides an immune cell storage solution, each 1000ml storage solution includes the following components and contents:

[0062] 55g of sucrose;

[0063] Glycerin 100ml;

[0064] Albumin 25g;

[0065] Sodium bicarbonate 7g;

[0066] Cholesterol 0.3g;

[0067] Propylene glycol 6% mg;

[0068] Vitamin E1g;

[0069] Vitamin C0.7mg;

[0070] 0.2% mg of EDTA;

[0071] Phosphate buffer is 75% ml;

[0072] Sodium selenite is 0.2% mg;

[0073] The balance is cell culture medium.

Embodiment 3

[0075] The invention provides an immune cell storage solution, each 1000ml storage solution includes the following components and contents:

[0076] 75g of sucrose;

[0077] Glycerin 150ml;

[0078] Albumin 30g;

[0079] Sodium bicarbonate 10g;

[0080] Cholesterol 0.4g;

[0081] Propylene glycol 8% mg;

[0082] Vitamin E1.1g;

[0083] Vitamin C0.9mg;

[0084] EDTA at 0.4% mg;

[0085] Phosphate buffer is 95% ml;

[0086] Sodium selenite is 0.5% mg;

[0087] The balance is cell culture medium.

[0088] In three embodiments, the effect of each composition is as follows:

[0089] Albumin mainly regulates the dynamic balance of water in cells, maintains the osmotic pressure of cell solution, and is also a non-permeable cryoprotectant.

[0090] Propylene glycol (1,2-Propanediol, Propylene Glycol), a permeable cryoprotectant, can diffuse through the cell membrane and penetrate into the cell, balance the osmotic pressure inside and outside the cell, reduce the concentration...

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PUM

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Abstract

The invention provides an immune cell storage solution, wherein every 1000 ml of the immune cell storage solution comprises the following components by weight: 25-75 g of sucrose, 50-150 ml of glycerol, 20-30 g of albumin, 5-10 g of sodium bicarbonate, 0.2-0.4 g of cholesterol, 5-8% mg of propylene glycol, 0.6-1.1 g of vitamin E, 0.6-0.9 mg of vitamin C, 0.01-0.4%mg of EDTA, 65-95% ml of a phosphate buffer solution, 0.01-0.5% mg of sodium selenite, and the balance of a cell culture medium. According to the invention, the storage solution can still maintain the cell motility rate of 80% or above and the adherence rate of 70% or above after 48 h during storage or transportation, can meet the short-distance transportation requirement of fresh mesenchymal stem cells, is safe to human bodies byusing clinical drugs with clear components, can be directly injected after being recovered to the room temperature, and greatly facilitate the clinical application of the mesenchymal stem cell preparation after short-distance transportation.

Description

technical field [0001] The invention relates to the field of immune cell storage, in particular to an immune cell storage solution and its preparation and application method. Background technique [0002] Immune cells are a type of cells involved in immune response or immune response, mainly including lymphocytes, monocytes, macrophages and dendritic cells, collectively referred to as peripheral blood mononuclear cells. They play an important role in stabilizing the health and well-being of the body in the human body, and they are the role of the body's immune defense, immune stability and immune surveillance. [0003] In recent years, tumor and virus immunotherapy techniques have been applied clinically, and the immunity of patients can be improved by stimulating, culturing, expanding and reinfusing autologous immune cells in vitro. However, after the body's immunity reaches its peak at the age of 20, the immune system gradually declines with age. At the age of 40, the imm...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/078
CPCA01N1/021C12N5/0634
Inventor 刘俊年刘成龙王丹李静管元宁王艺霏
Owner 青岛中德华大细胞科技有限责任公司
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