Application method of chitosan/glucan-containing moss sporophyte suspension to bare land greening
An application method and suspension technology, applied in the directions of glucan adhesive, application, horticultural method, etc., can solve the problems of failure to cover green, poor adhesion of moss, irritating odor, etc., and achieve high coverage and stickiness. The effect of good bonding effect, improving adhesion and air permeability
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Embodiment 1
[0028] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:
[0029] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 3% w / w peroxide to the spores Hydrogen solution, after shaking for 6 minutes, obtain a mixed solution of sterilized spores and hydrogen peroxide; the volume ratio of the spores to 3% w / w hydrogen peroxide solution is 1:10;
[0030] Step 2, inoculate the mixed solution of 2 mL of sterilized spores and hydrogen peroxide into 10 mL of spore germination induction medium, carry out spore germination induction culture for 30 days, and obtain a suspension containing spores and protonema; the spore germination induction culture conditions are: Temperature 22 ℃, light intensity 2500lx, the li...
Embodiment 2
[0038] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:
[0039] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 4% w / w peroxide to the spores Hydrogen solution, after shaking for 10 minutes, a mixture of sterilized spores and hydrogen peroxide was obtained; the volume ratio of the spores to 4% w / w hydrogen peroxide solution was 1:10;
[0040] Step 2, inoculate the mixed solution of 2mL sterilized spores and hydrogen peroxide into 10mL spore germination induction medium, carry out spore germination induction culture for 50 days, obtain the suspension containing spores and protonema; the spore germination induction culture conditions are: The temperature is 28°C, the light intensity is 3000lx, t...
Embodiment 3
[0048] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:
[0049] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 3.5% w / w peroxide to the spores Hydrogen solution, after shaking for 8 minutes, obtain a mixed solution of sterilized spores and hydrogen peroxide; the volume ratio of the spores to 3.5% w / w hydrogen peroxide solution is 1:10;
[0050] Step 2, inoculate the mixed solution of 2 mL of sterilized spores and hydrogen peroxide into 10 mL of spore germination induction medium, carry out spore germination induction culture for 40 days, and obtain a suspension containing spores and protonema; the spore germination induction culture conditions are: Temperature 25 ℃, light intensity 2800lx, th...
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