Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Maize sheath surface paenibacillus polymyxa strain and application thereof

A technology of Paenibacillus polymyxa and leaf sheaths, which is applied to the field of Paenibacillus polymyxa strains on the surface of corn leaf sheaths, can solve the problems of few biological control methods and achieve obvious control effects

Active Publication Date: 2021-02-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods of preventing fungal diseases of corn still rely on agricultural control, planting resistant varieties and chemical control methods, and there are few biological control methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Maize sheath surface paenibacillus polymyxa strain and application thereof
  • Maize sheath surface paenibacillus polymyxa strain and application thereof
  • Maize sheath surface paenibacillus polymyxa strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Isolation and identification of Paenibacillus polymyxa SF05

[0015] The bacterial strain Paenibacillus polymyxa SF05 of the present invention is separated from the corn leaf sheath surface, and concrete operation method is as follows:

[0016] (1) take out the corn leaf sheath tissue that sheath blight symptom occurs, cut into 3 * 3mm size with scalpel;

[0017] (2) Disinfect the surface of the tissue in 70% alcohol for 5 seconds, rinse with sterile water for 15 seconds, and repeat three times;

[0018] (3) Dry the surface moisture of the tissue with sterile absorbent paper;

[0019] (4) Place the leaf sheath tissue in the center of the PDA plate, place the plate at 28°C, and incubate in an incubator for 2 days;

[0020] (5) White mycelium grows around the tissue, but some culture medium has a zone of inhibition, pick the bacteria in the center of the zone of inhibition and purify it on a new LB plate;

[0021] (6) Pick and isolate the biocontrol colon...

Embodiment 2

[0027] Combined with the above-mentioned 16S rDNA sequencing, the bacterium was identified as Paenibacillus polymyxa, named SF05, and was preserved in the China Center for Type Culture Collection, with the starting date of preservation being July 31, 2020, and the preservation number: M 2020384 , Deposit address: Wuhan University, Wuhan, China. Embodiment 2: Paenibacillus polymyxa SF05 antibacterial activity assay

[0028] Paenibacillus polymyxa SF05 bacteriostatic activity assay of the present invention adopts antagonistic culture method, and concrete operation is as follows:

[0029] (1) Pick fresh Paenibacillus polymyxa SF05 colony in fresh LB liquid culture medium, shake culture at 28°C to a concentration of about 1×10 8 CFU / ml;

[0030] (2) Place a pathogenic fungus block with a diameter of 5mm in the center of the PDA flat plate, get 10 μl of bacterial liquid and drop it on the four corners of the place 2cm away from the block. The pathogenic fungi used are mainly Rhiz...

Embodiment 3

[0034] Embodiment 3: the growth-promoting effect of Paenibacillus polymyxa SF05 to seedling stage maize

[0035] Paenibacillus polymyxa SF05 of the present invention mainly adopts spraying method to the mensuration of seedling stage corn growth promoting effect, concrete operation is as follows:

[0036] (1) Pick fresh Paenibacillus polymyxa SF05 colonies in fresh LB liquid medium, shake culture at 28°C to a concentration of 1×10 8 CFU / ml;

[0037] (2) Spray the three-leaf stage corn seedlings (variety: Zhetian 20) with the bacterium liquid with a sprayer until the bacterium liquid droplets evenly adhere to all the leaves and stems of the corn, but do not form small trickles to flow down;

[0038] (3) LB liquid culture medium is processed in the same way with a sprayer, and simultaneously grows seedlings of the same kind of corn seedlings that grow similarly, as a contrast;

[0039] (4) After 16 days of cultivation in the greenhouse, the fresh weight and plant height of abov...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biological prevention and control of maize diseases, and discloses a maize sheath surface paenibacillus polymyxa strain and application thereof. The maize sheath surface paenibacillus polymyxa strain disclosed by the invention is paenibacillus polymyxa SF05; the strain is collected in China Center for Type Culture Collection on July 31, 2020, the collection number is CCTCC NO: M 2020384, and the collection address is Wuhan University, Wuhan, China. The maize sheath surface paenibacillus polymyxa strain can effectively inhibit the growth of rhizoctonia solani, exserohilum turcicum, bipolaris maydis and fusurium verticillioides, and can induce up-regulation of the expression level of maize disease resistance-related genes, enhances maize disease resistance, and can effectively control the maize sheath blight in production.

Description

technical field [0001] The invention relates to the technical field of biological control of corn diseases, in particular to a strain of Paenibacillus polymyxa on the surface of corn leaf sheaths and an application thereof. Background technique [0002] Maize (Zea mays L.) is an important food crop in my country. It is mainly planted in the mountainous areas of Northeast, North and Southwest China, and it is also partially planted in the coastal areas of Jiangsu and Zhejiang. The occurrence of maize diseases directly affects the yield and quality of maize. Common fungal diseases on corn include corn sheath blight, large leaf spot, small spot and ear rot, etc. The occurrence of these diseases has brought serious losses to corn production. Rhizoctonia solani (Rhizoctonia solani), large spot Exserohilum turcicum, Bipolaris maydis and Fusurium verticillioides can cause the above four diseases on maize respectively. Among them, corn sheath blight usually damages the leaf sheath...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P21/00A01P3/00C05F11/08C05G3/60C12R1/01
CPCC12N1/20A01N63/25C05F11/08C05G3/60C12R2001/01C12N1/205
Inventor 陈斌赵福成韩海亮侯俊峰谭禾平包斐王桂跃
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com