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Composite microbial preparation for fermented feed and fermented expanded pellet feed

A technology of extruded pellet feed and compound microorganisms, applied in animal feed, animal feed, application, etc., can solve the problems of poor probiotic effect and easy inactivation of probiotics, and achieve strong degradation ability, good feeding effect, and acid production capacity strong effect

Pending Publication Date: 2021-02-05
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a compound microbial preparation and Its fermented extruded pellet feed

Method used

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  • Composite microbial preparation for fermented feed and fermented expanded pellet feed
  • Composite microbial preparation for fermented feed and fermented expanded pellet feed
  • Composite microbial preparation for fermented feed and fermented expanded pellet feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation, screening and gene sequencing of Lactobacillus salivarius LS strain

[0031] Source of strains: intestinal contents of healthy grass carp (aquaculture base of Jiangxi Agricultural University).

[0032] Method: The healthy grass carp was dissected in an ultra-clean workbench, and its intestinal contents were directly diluted 10 times in sterile saline to 10 -2 -10 -8 Concentration, take 0.1ml and spread on the MRS solid medium containing 1% calcium carbonate, and culture at 30°C for 24-48h. Pick a single colony that produces a calcilytic circle from the MRS solid medium containing 1% calcium carbonate and purify it on an ordinary MRS solid medium. After purification twice, carry out Gram staining and biochemical identification. The colony was inoculated in the corresponding liquid medium, and cultured at 30°C and 180r / min for 24-48h.

[0033] A single strain in the MRS liquid medium was planted on the MRS solid medium with 1% calcium carbonate by...

Embodiment 2

[0038] Example 2: Isolation, screening and gene sequencing of Bacillus subtilis B1 strain

[0039] Sources of strains: termite intestinal material (termites collected from the campus forest of Jiangxi Agricultural University); intestinal contents of healthy grass carp (aquaculture base of Jiangxi Agricultural University); a strain of Bacillus subtilis and a strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) , two strains have antibacterial effect on Aeromonas hydrophila (Aeromonas hydrophila), isolated and preserved by our laboratory.

[0040] Indicator bacteria: Aeromonas hydrophila AH-S1, Aeromonas sobria (Aeromonas sobria) AS-S2, isolated from diseased eel liver, preserved by our laboratory.

[0041] Medium:

[0042] Guaiacol primary screening medium: yeast extract 10g / L, glucose 20g / L, guaiacol 2.5g / L, agar 15g / L, adjust pH to 7.0, autoclave at 121°C for 20min.

[0043] RB brilliant blue medium: RB brilliant blue 0.03g / L, yeast extract 5g / L, tryptone 10g / L...

Embodiment 3

[0061] Embodiment 3: the composite ratio optimization of three strains of bacteria in the composite microbial preparation

[0062] Compound microbial preparations:

[0063] A strain of Bacillus subtilis (Bacillus subtilis) B1 capable of degrading lignin, isolated from termite guts, obtained through separation and screening in Example 2, with a preservation number of CGMCC NO.20405;

[0064] A Lactobacillus salivarius LS strain, isolated from the intestinal contents of healthy grass carp, was obtained by isolation and screening in Example 1, and the preservation number is CGMCC NO.14458;

[0065] A strain of Saccharomyces cerevisiae (Saccharomyces cerevisiae) J1 was purchased from the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.2.388.

[0066] Feed formula:

[0067] Feed raw materials and processing and granulation were provided by Dayou Nong Biotechnology Co., Ltd. The test feed was extrude...

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Abstract

The invention relates to the technical field of fermented feeds, and particularly discloses a composite microbial preparation for a fermented feed and a fermented expanded pellet feed. The composite microbial preparation consists of three bacterial liquid composite proportions of a self-screened and preserved bacillus subtilis B1 strain with the preservation number of CGMCC NO.20405, a self-screened and preserved lactobacillus salivarius LS strain with the preservation number of CGMCC NO.14458 and a saccharomyces cerevisiae J1 strain with the preservation number of CGMCC NO.2.388 purchased from a preservation center. The composite microbial preparation is used for preparing the fermented expanded pellet feed. According to the fermented expanded pellet feed, a preparation process of firstlyprocessing and granulating the feed, then carrying out composite fermentation by using the composite microbial preparation and not needing to process and granulate again after fermentation is adopted, the inactivation of viable bacteria is avoided, and the loss of nutrient substances in the processing process is also reduced. The feed has the characteristics of good metabolite, sufficient nutritional value, high viable organism content, good feeding effect and the like.

Description

technical field [0001] The invention belongs to the technical field of fermented feed, in particular to a compound microbial preparation for fermented feed and fermented puffed granular feed. Background technique [0002] Probiotics generally refer to a type of live bacteria that can survive in the intestinal tract of animals and are beneficial to animal health. With the advent of the "antibiotic-free" era, the use of probiotics is becoming more and more widespread. Probiotics can synthesize and secrete some amino acids and vitamins needed by animals in the intestinal tract of the host, and can also secrete some proteases, cellulose, etc. Enzymes and amylases can promote the digestion and absorption of feed by animals, improve feed utilization and reduce feed coefficient; they can also improve the body's immunity and maintain the balance of intestinal flora structure. Among them, Bacillus sp., lactobacillus and saccharomyce are several micro-ecological strains widely used i...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/12A23K10/22A23K10/37A23K10/30A23K10/20A23K20/158A23K20/26A23K20/10A23K20/147A23K50/80A23K50/75
CPCA23K10/18A23K10/12A23K10/22A23K10/37A23K10/30A23K10/20A23K20/158A23K20/26A23K20/10A23K20/147A23K50/80A23K50/75Y02P60/87Y02A40/818
Inventor 张锦华周秋白王颖
Owner JIANGXI AGRICULTURAL UNIVERSITY
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