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Complete sequence of porcine BMP7 gene 3'-UTR, mutation sites associated with reproductive traits and application of mutation sites

A mutation site, -UTR technology, applied in the field of molecular biology, can solve the problem of 3'-UTR not being identified, and achieve the effect of accelerating the progress of breeding

Pending Publication Date: 2021-02-02
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, the 5′-UTR and coding sequence of porcine BMP7 gene have been cloned and identified, but the 3′-UTR has not yet been identified

Method used

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  • Complete sequence of porcine BMP7 gene 3'-UTR, mutation sites associated with reproductive traits and application of mutation sites
  • Complete sequence of porcine BMP7 gene 3'-UTR, mutation sites associated with reproductive traits and application of mutation sites
  • Complete sequence of porcine BMP7 gene 3'-UTR, mutation sites associated with reproductive traits and application of mutation sites

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Effect test

Embodiment 1

[0025] 1. Experimental materials

[0026] A total of 331 healthy Large White sows were randomly selected from the core group of a certain pig farm, and the number of litters was recorded. Ear tissue samples were collected with ear size forceps and stored at -20°C for extraction of genomic DNA. Fresh ovaries of pigs were collected and follicles were isolated for extraction of total RNA.

[0027] 2. Nucleic acid extraction

[0028] Genomic DNA of pig ear tissue was extracted by conventional phenol / chloroform extraction method and stored at -20°C. Total follicle RNA was extracted by the Trizol method, reverse-transcribed into cDNA using a reverse transcription kit (Takara Company), and stored at -20°C.

[0029] 3. Identification of 3′-UTR of porcine BMP7 gene

[0030] Using 3'-RACE technology to identify the 3'-UTR sequence of porcine BMP7 gene, the specific operation is as follows The steps in the RACE5' / 3' kit (TaKaRa Company) instructions were carried out. First, the RA...

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Abstract

The invention discloses a complete sequence of a porcine BMP7 gene 3'-UTR, mutation sites associated with reproductive traits and application of the mutation sites. The two mutation sites significantly associated with the porcine reproductive traits are located at the 273rd site and the 955th site of the porcine BMP7 gene 3'-UTR respectively, wherein the first site is A>G mutation named as c.1569A>G, and the second mutation is insertion / deletion mutation named as c.2251indelCTGGGGT. The sow reproductive traits are low-heredity traits; the genetic progress is relatively slow due to the adoptionof a conventional breeding technology; due to the fact that the combined genotype of the mutation sites is remarkably associated with the total litter size of a sow and is remarkably close to the live litter size, the two sites are detected by adopting a simple and convenient genotyping technology, so that high-yield genotype individuals can be selected and remained, and low-yield genotype individuals are eliminated; and therefore the mutation sites have important economic value for selecting and remaining the high-yield individuals and improving the reproductive performance of sow groups.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to the complete sequence of the 3'-UTR of the pig BMP7 gene, mutation sites associated with reproductive traits and applications thereof. Background technique [0002] Bone morphogenetic protein 7 (BMP7) is an important member of the TGF-β superfamily BMP subfamily, widely expressed in various tissues and organs of mammals, and involved in the regulation of various important physiological and pathological processes . In female mammals, BMP7 is necessary to maintain the normal functions of reproductive-related tissues and organs such as the hypothalamus-pituitary system, ovary, fallopian tube, and uterus. In ovarian tissue, BMP7 is mainly secreted by theca cells and can regulate various ovarian functions. In follicle cells cultured in vitro, inhibiting BMP7 can induce bovine theca cells to inhibit androgen secretion, while adding BMP7 fusion protein can stimulate buffalo ovarian gran...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李齐发尹杭杜星潘增祥
Owner NANJING AGRICULTURAL UNIVERSITY
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