Paenibacillus polymyxa LCP-01, complex microbial inoculant compounded by paenibacillus polymyxa LCP-01 and bile acid and application of complex microbial inoculant

A technology of polymyxa spores and compound bacterial agents, which is applied in the field of microorganisms, can solve the problems of narrow sales channels for meat ducks, many diseases of meat ducks, and market fluctuations, and achieve the effects of protecting liver and intestines, improving breeding efficiency, and reducing feed-to-meat ratio

Pending Publication Date: 2021-01-29
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] But at present, there are several risks in the breeding of meat ducks. First, the sales channels of meat ducks are re...

Method used

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  • Paenibacillus polymyxa LCP-01, complex microbial inoculant compounded by paenibacillus polymyxa LCP-01 and bile acid and application of complex microbial inoculant
  • Paenibacillus polymyxa LCP-01, complex microbial inoculant compounded by paenibacillus polymyxa LCP-01 and bile acid and application of complex microbial inoculant
  • Paenibacillus polymyxa LCP-01, complex microbial inoculant compounded by paenibacillus polymyxa LCP-01 and bile acid and application of complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening and identification of Paenibacillus polymyxa LCP-01

[0023] Take the soil as a sample, mix the soil and sterile water to make 10% of the soil -1 diluent, and then prepare 10 -2 、10 -3 、10 -4 、10 -5 Diluent. Take 10 respectively -3 、10 -4 、10 -5 Spread the diluted solution on nutrient agar medium (3.0g beef extract, 10.0g peptone, 5.0g NaCl, 20.0g agar, 1.0L distilled water, pH 7.0), culture at 37°C for 48 hours, and select obvious colonies for multiple purifications A single colony was obtained and named LCP-01.

[0024] Such as figure 1 As shown, the colony of the bacterial strain LCP-01 on the nutrient agar medium is round, sub-round or oval, beige, 3-5mm in diameter, flat, smooth and matte, opaque, with irregular wavy edges And has a translucent border.

[0025] The genomic DNA of the bacterial strain LCP-01 was extracted, and used as a template, PCR amplification was performed using 16S rRNA universal primers to obtain the 16S rRNA a...

Embodiment 2

[0027] Embodiment 2: the preparation of composite bacterial agent

[0028] 1. Preparation of bile acids

[0029] The preparation process steps of described bile acid are as follows:

[0030] (1) Saponification: Add 1 kg of pig gall powder crushed through a 40-mesh sieve to the reaction kettle, then add 10L of sodium hydroxide solution with a mass concentration of 10%, heat and stir until boiling, and keep stirring for 14 hours in a boiling state, and then react The generated saponified liquid is cooled to solid-liquid separation, the supernatant is removed, and the remaining solid is bile acid saponified product;

[0031] (2) Decolorization: Add 6L of water to the bile acid saponification product, heat to 80°C to completely dissolve the bile acid saponification product, then pour it into a decolorization tank, add 300mL hydrogen peroxide and stir evenly, react at room temperature for 24 hours, and then put the reaction The solution is filtered into the acidification tank to ...

Embodiment 3

[0042] A meat duck farm in Zhejiang received 10,000 chickens, and 400 of them were randomly selected for the effect test of the compound bacterial agent. The 500g composite bacterial agent prepared in Example 2 is uniformly dissolved in 4500g water to make a composite bacterial solution, wherein 200 meat ducks drink the composite bacterial solution for 8 hours every day; another 200 meat ducks are used as a control group and drink at the same time every day The same amount of clear water, and the others were carried out according to conventional breeding. After drinking for 7 days, the growth of the two groups of meat ducks was observed.

[0043]The results are shown in Table 1. Compared with the control group, the average body weight of the meat ducks drinking the compound bacterial solution increased more after 7 days, and the meat ducks suffered from less hepatitis and had stronger vitality. At this time, the meat duck is in the stage of liver transformation, and it is easy...

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Abstract

The invention discloses paenibacillus polymyxa LCP-01, a complex microbial inoculant compounded by the paenibacillus polymyxa LCP-01 and bile acid and application of the complex microbial inoculant. The classification name of the paenibacillus polymyxa LCP-01 is Paenibacillus polymyxa, the paenibacillus polymyxa LCP-01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the paenibacillus polymyxa LCP-01 is CGMCC No.20819; and the bacterial colony of the bacterial strain is round, quasi-round or oval, beige, smooth and lusterless in surface and opaque, the diameter is 3-5 mm, and the edge of the bacterial colony is in an irregular wavy shape and is provided with a semitransparent edging. The invention further provides a complex microbial inoculant prepared by compounding paenibacillus polymyxa LCP-01 bacterial powder and bile acid. The complex microbial inoculant can effectively remove maternal toxins of meat ducklings, protect livers and intestines of the meat ducks, can promote growth of the meat ducks, can increase the slaughter weight of the meat ducks, can reduce the feed conversion ratio and can improve the breeding benefits of the meat ducks.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Paenibacillus polymyxa LCP-01 and a compound microbial agent compounded with bile acid and its application. [0002] technical background [0003] The population of our country is huge, and meat ducks are eaten in a variety of ways and the meat is firm. Therefore, the demand for meat ducks in our country is also relatively large, and meat duck breeding has become a hot spot in the breeding industry. Moreover, the meat duck breeding cycle is short, and it can be raised many times a year, which also makes the meat duck gradually move towards large-scale farming. [0004] But at present, there are several risks in the breeding of meat ducks. First, the sales channels of meat ducks are relatively narrow, which is likely to cause large fluctuations due to the market. Second, there are many diseases of meat ducks. die. For example, in the early stage of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/75A23K10/18A23K20/158C12R1/01
CPCA23K10/18A23K20/158A23K50/75C12N1/20C12N1/205C12R2001/01
Inventor 娄倩倩籍立民曹爱智
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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