New application of palmitic acid as anti-hepatocarcinoma sensitizer of methylselenic acid

A technology of methyl selenic acid and palmitic acid, which is applied in the field of medicine, can solve the problems of synergism, and achieve the effect of anti-liver cancer treatment synergy, enhanced induction of liver cancer cell apoptosis, and good effect of lipotoxicity

Active Publication Date: 2021-09-07
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report that palmitic acid has a synergistic effect on the apoptosis of cancer cells induced by methylselenoic acid

Method used

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  • New application of palmitic acid as anti-hepatocarcinoma sensitizer of methylselenic acid
  • New application of palmitic acid as anti-hepatocarcinoma sensitizer of methylselenic acid
  • New application of palmitic acid as anti-hepatocarcinoma sensitizer of methylselenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. The effect of palmitic acid on increasing the anti-liver cancer efficacy of methylselenic acid in mice.

[0025] Test method: 12 male BALB / C nude mice (Beijing Weitong Lihua Experimental Technology Co., Ltd.), 6-8 weeks old, 2 × 10 6 A HepG2 cell was mixed with an equal volume of Matrigel, injected subcutaneously into the right side of the mouse, and the tumor volume was measured with a vernier caliper. When the tumor volume is about 100-120mm 3 , the mice were randomly divided into control group, methyl selenic acid treatment group, palmitic acid treatment group and combined treatment group. The corresponding solvent was administered to the control group, the methyl selenic acid treatment group was intragastrically administered at a dose of 2 mg / kg per day, and the palmitic acid treatment group was intragastrically administered at a daily dose of 10.26 mg / kg. Mouse body weight and tumor volume were measured every other day. After the end of the experiment...

Embodiment 2

[0028] Embodiment 2, palmitic acid increases the apoptosis of HepG2 human liver cancer cells induced by methyl selenic acid

[0029] Human liver cancer cell line HepG2 cells were treated with MSeA at a low dose concentration of 2 μM for 24 hours, then 100 μM or 200 μM PA was added, and the number of apoptotic cells was detected by Annexin V-FITC / PI double staining method. Figure 4 The results showed that the number of cells treated with 2μMMSeA was not significantly different from that of the control group, the addition of PA induced the apoptosis of HepG2 cells, and the combined treatment group significantly caused cell death. The results showed that palmitic acid could increase the apoptosis of HepG2 human hepatoma cells induced by methylselenoic acid.

Embodiment 3

[0030] Example 3, methyl selenic acid prevents lipapoptosis of L02 human normal liver cells caused by palmitic acid

[0031] Using palmitic acid-treated L02 normal human liver cells as a model, the number of apoptotic cells was detected by crystal violet. After pretreatment of L02 cells with 2 μM MSeA for 24 h, corresponding concentrations of palmitic acid were added. Figure 5 The results shown indicated that the addition of MSeA significantly restored cell viability. The results showed that methyl selenic acid could prevent the apoptosis of L02 human normal liver cells induced by palmitic acid.

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Abstract

The invention provides a new application of palmitic acid as an anti-hepatoma sensitizer of methyl selenic acid. The invention provides a new application of combining palmitic acid and methyl selenic acid in treating liver cancer. The present invention reveals that palmitic acid has the effect of enhancing the apoptosis of liver cancer cells induced by methyl selenic acid, has a good synergistic effect on the anti-liver cancer treatment of methyl selenic acid, and that methyl selenic acid may have a potential lipotoxic effect on palmitic acid Has a good preventive effect. The invention provides a scientific basis for developing anti-liver cancer drugs based on palmitic acid and methyl selenic acid, and has great application value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the new use of palmitic acid as a methyl selenic acid anti-hepatoma sensitizer, that is, the new use of palmitic acid to enhance the anti-liver cancer effect of methyl selenic acid. Background technique [0002] Methylselenic acid (MSeA) is an organic compound of selenium, which can be directly reduced to methyl by glutathione and reduced nicotinamide adenine nucleotide phosphate (NADPH), which are widely present in tumor cells. Selenium has a strong anti-cancer effect. Methylselenoate induces a comprehensive redox reaction to modify proteins, and these changes are caused by intracellular stress caused by unfolded or misfolded proteins. [0003] Palmitic acid (PA) is a 16-carbon long-chain saturated fatty acid, which is the most abundant saturated fatty acid in the human body. Palmitic acid is not only an energy source, but also acts as a signaling molecule to regul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/20A61K31/185A61P35/00A61P1/16
CPCA61K31/185A61K31/20A61P1/16A61P35/00A61K2300/00
Inventor 赵冲张涵尹淑涛扈洪波
Owner CHINA AGRI UNIV
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