Culture method for increasing content of crude polysaccharide in cordyceps militaris sporocarp

A cultivation method and crude polysaccharide technology, applied in the cultivation field of increasing the crude polysaccharide content in the fruiting body of Cordyceps militaris, can solve the problems of reducing energy consumption, unpredictability, shortening the production cycle, etc., and achieve favorable promotion, superior quality, and increased trust Effect

Active Publication Date: 2021-01-29
江苏康能生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent document publicly reports that the extruded raw material can be used on the medium of Cordyceps militaris, compared with the technical features of this technical solution, the former plays the role of reducing energy consumption, shortening the production cycle and improving the biotransformation rate, etc., and the latter plays the role of To "surprisingly greatly increase" the content of crude polysaccharides, the roles played by the two and the technical problems solved are different, which does not constitute a situation of technical inspiration, and this patent document does not solve the problem of crude polysaccharides from the fruiting body of Cordyceps militaris. content and other issues to disclose specific technical solutions, both of which also have obvious distinguishing technical features; in addition, combined with reference to the data of the control group of this technical solution, relying on part of the information in the prior art of this patent document is also limited to predicting Cordyceps militaris The content of crude polysaccharides in the fruiting bodies has "increased to a certain extent", and it is impossible to foresee the technical effect of this technical solution on the basis of conditions such as puffed medium cultivation and ultraviolet sterilization, or on the basis of simple test adjustments, to greatly increase the content of crude polysaccharides , therefore, the technical effect achieved by this technical solution on the basis of the control group is not obvious

Method used

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  • Culture method for increasing content of crude polysaccharide in cordyceps militaris sporocarp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of liquid nutrient solution and bacterial solution

[0023] Prepare liquid nutrient solution according to the following components: glucose 10g / L, magnesium sulfate 1.5g / L, potassium dihydrogen phosphate 0.8g / L, water 1L, after bottling, sterilize with damp heat for 30min at a temperature of 121°C, and after cooling According to the mass ratio of 1:10-15, the Cordyceps militaris liquid strain is mixed with the above-mentioned cooled liquid nutrient solution to obtain the bacterial liquid;

[0024] (2) Preparation and inoculation of medium

[0025] Weigh 40g of the puffed solid material, put it into the cultivation carrier, and sterilize it with damp heat for 30min at a temperature of 121°C; after cooling, inoculate the bacterial liquid evenly into the in solid media;

[0026] (3) Cultivation and management

[0027] The culture medium after inoculation was cultured in the dark at a temperature of 14-16°C and a humidity of 50-60% for 2-3 days. On the fo...

Embodiment 2

[0031] (1) Preparation of liquid nutrient solution and bacterial solution

[0032] Prepare liquid nutrient solution according to the following components: glucose 8g / L, magnesium sulfate 1.2g / L, potassium dihydrogen phosphate 0.5g / L, water 1L. According to the mass ratio of 1:10-15, the Cordyceps militaris liquid strain is mixed with the above-mentioned cooled liquid nutrient solution to obtain the bacterial liquid;

[0033] (2) Preparation and inoculation of medium

[0034] Weigh 30g of puffed solid raw material, put it into the cultivation carrier, and sterilize it with moist heat for 30min at a temperature of 121°C; after cooling, inoculate the bacterial liquid evenly into the in solid media;

[0035] (3) Cultivation and management

[0036] The culture medium after inoculation was cultured in the dark at a temperature of 14-16°C and a humidity of 50-60% for 2-3 days. On the fourth day, the color change treatment was carried out. At this time, the temperature was adjusted t...

Embodiment 3

[0040] (1) Preparation of liquid nutrient solution and bacterial solution

[0041] Prepare liquid nutrient solution according to the following components: glucose 5g / L, magnesium sulfate 1g / L, potassium dihydrogen phosphate 1g / L, water 1L. The mass ratio of 1:10-15 mixes the Cordyceps militaris liquid strain with the above-mentioned cooled liquid nutrient solution to prepare the bacterial liquid;

[0042] (2) Preparation and inoculation of medium

[0043] Weigh 20g of the expanded solid raw material, put it into the cultivation carrier, and sterilize it with damp heat for 30 minutes at a temperature of 121°C; after cooling, inoculate the bacterial liquid evenly into the in solid media;

[0044] (3) Cultivation and management

[0045] The culture medium after inoculation was cultured in the dark at a temperature of 14-16°C and a humidity of 50-60% for 2-3 days. On the fourth day, the color change treatment was carried out. At this time, the temperature was adjusted to 18-20°...

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Abstract

The invention relates to a culture method for increasing the content of crude polysaccharide in cordyceps militaris sporocarp. The culture method comprises the following steps of using puffed rice andcorn or other puffed grain raw materials as a solid culture medium, using a nutrient solution as a liquid culture medium, conducting respective and independent sterilizing, conducting proportional and uniform mixing after cooling, carrying out inoculation, dark culture, coloring treatment and other procedures, then in the growth period of the cordyceps militaris sporocarp on the 30th-35th day, adjusting the temperature from 18-20 DEG C to 16-18 DEG C, adjusting the illumination intensity from 100-500 Lux to 20-50 Lux, maintaining the humidity at 60%-80%, and continuously culturing to the 52th-55th day for harvesting, so that the content of the crude polysaccharide in cordyceps militaris is obviously increased by 254.9%, the technical effect is significantly unexpected, and the culture method has important significance for industrialization of the high-quality cordyceps militaris.

Description

technical field [0001] The invention relates to the field of cultivation of edible and medicinal fungi, in particular to a cultivation method for increasing the content of crude polysaccharide in the fruit body of Cordyceps militaris. Background technique [0002] Cordyceps militaris ( Cordyceps militaris (L.) Fr.), also known as Cordyceps militaris and Cordyceps militaris, is a commonly used edible and medicinal fungus, belonging to Ascomycotina (Ascomycotina), Ergotaceae (Clavicipitaceue), Cordyceps ( Cordyceps ), which belong to the same fungus as Cordyceps sinensis. It is rich in nutrients, and has the functions of protecting the lungs, kidneys, and strengthening the body. It has gradually become an ideal substitute for Cordyceps sinensis. [0003] In recent years, the medicinal value and health care function of Cordyceps militaris have been widely accepted by people, and with the discovery of Wang Chengshu's research team at Shanghai Institute of Plant Physiology and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 汪小慧窦小佳王琨夏振荣
Owner 江苏康能生物工程股份有限公司
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