Culture medium for continuously preparing lentinus edodes fermentation liquid seeds and preparation method

Abstract

The invention discloses a culture medium for continuously preparing lentinus edodes fermentation liquid seeds and a preparation method. The culture medium contains arginine salt or arginine and soybean lecithin. The culture medium comprises the following components including, by weight (W / W), 20 to 30 percent of potatoes, 1.5 to 2.5 percent of glucose, 0.2 to 1.5 percent of the arginine salt or arginine, 0.05 to 0.35 percent of monopotassium phosphate, 0.05 to 0.20 percent of magnesium sulfate, 0.3 to 1.0 percent of the soybean lecithin, 0.0001 to 0.0005 percent of biotin, 0.005 to 0.02 percent of vitamin B1 and the balance of water, wherein the sum of the percentages is 100%. According to the culture medium, the arginine salt or arginine is added into the formula to promote growth of lentinus edodes mycelia, and the soybean lecithin is selectively added into the formula to increase oxygen supply to immobilized mycelia. Meanwhile, the fermentation culture time is shortened, the seed production period is shortened, the inoculation pollution risk is reduced, and the operation is simple and convenient.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a continuous preparation medium for fermenting liquid seeds of Lentinus edodes and a preparation method. Background technique [0002] At present, the literature on "Polyurethane foam immobilized Ganoderma lucidum repeated batch fermentation to produce exopolysaccharide (WanAbd Al Qadr Imad Wan-Mohtar, Roslinda Abd Malek, Linda M.Harvey, Brian McNeil. Exopolysaccharide production by Ganoderma lucidum immobilized onpolyurethane foaminare peated-batch Fermentation, Biocatalysis and Agricultural Biotechnology 8 (2016) 24-31)" studied the immobilization and repeated batch fermentation of the large edible fungus Ganoderma lucidum to produce exopolysaccharides. Literature on "Production of exopolysaccharide by Ganoderma lucidum in a repeated-batchfermentation. Biocatalysis and Agricultural Biotechnology, Volume 6 , April 2016, Pages 91-101) "Research on the use of poly...

AI Technical Summary

Problems solved by technology

[0003] Although Lentinus edodes and Ganoderma lucidum are both large edible fungi, there are great differences in the actual fermentation culture. The culture medium formula mentioned above cannot be directly applied or used as a common-sense replacement and adjustment for professionals in the general field.

[0004] If the formula of the reference literature is directly applied, after the mushrooms are fixed on the polyurethane foam, the mushroom mycelium is weak, the toughness is poor, it is easy to age, the number of passages is limited, it is difficult to repeat multiple cultures, and it is not suitable for the growth of mushrooms

Simultaneously, after Lentinus edodes is fixed on the polyurethane foam plastics, the free mycelium biomass in the fermented liquid is low, not stout, and the growth rate is slow, it is difficult to be used as the liquid strain of next stage fermentation